The effect of 4-chloroindole-3-acetic acid on some growth parameters in mung bean under cadmium stress

Seedlings of mung bean (Vigna radiata L. Wilczek) cv. T-44 grown on a sand culture were exposed to cadmium (Cd) (100 µM) on day 10 of growth. These seedlings were subsequently sprayed with deionized water (control) or 4-chloroindole-3-acetic acid (4-Cl-IAA) (10-6 M) on day 15 and were sampled 30 days after sowing to assess growth and metabolite content. Growth (length, and fresh and dry mass of roots and shoots) and the level of metabolites (chlorophyll, nitrogen, phosphorus, total sugar, and soluble protein) in the leaves significantly decrease in response to Cd stress; however, the 4-Cl-IAA (10-6 M) treatment, both in the absence and presence of Cd, significantly increased the parameters studied. The response generated in the plants grown under stress-free conditions was more prominent, as compared in those exposed to Cd.

The effect of 4-chloroindole-3-acetic acid on some growth parameters in mung bean under cadmium stress

Seedlings of mung bean (Vigna radiata L. Wilczek) cv. T-44 grown on a sand culture were exposed to cadmium (Cd) (100 µM) on day 10 of growth. These seedlings were subsequently sprayed with deionized water (control) or 4-chloroindole-3-acetic acid (4-Cl-IAA) (10-6 M) on day 15 and were sampled 30 days after sowing to assess growth and metabolite content. Growth (length, and fresh and dry mass of roots and shoots) and the level of metabolites (chlorophyll, nitrogen, phosphorus, total sugar, and soluble protein) in the leaves significantly decrease in response to Cd stress; however, the 4-Cl-IAA (10-6 M) treatment, both in the absence and presence of Cd, significantly increased the parameters studied. The response generated in the plants grown under stress-free conditions was more prominent, as compared in those exposed to Cd.

___

  • Prasad MNV. Cd toxicity and tolerance in vascular plants. Environ Exp Bot 35: 525-545, 1995. 2. Hall JL. Cellular mechanisms for heavy metal detoxification and tolerance. J Expt Bot 53: 1-11, 2002. 3. Van Assche F, Clijisters H. Effect of metals on enzyme activity in plant. Plant Cell Environ. 13, 195-206, 1990. 4. Normanly J. Auxin metabolism. Physiol Plant 100: 431-442, 1997. 5.
  • Engvild KC. The chloroindole auxins of pea, strong plant
  • growth5 hormones or Endogenous Herbicides? Riso National
  • Laboratory, Denmark, 1994. 6.
  • Ernstsen A, Sandberg G. Identification of 4-chloroindole-3
  • acetic acid and indole-3-aldehyde in seeds of Pinus sylvestris.
  • Physiol Plant 68: 511-518, 1986. 7.
  • Reinecke DM. 4-Chloro-indole-3-acetic acid and plant growth.
  • Plant Growth Regul 27: 3-13, 1999. 8. Ali B, Hayat S, Fariduddin Q, et al. 4-chloroindole-3-acetic acid: Metabolism and bioactivity in plants, In Hemataranjan, A. (ed.) Advances in plant physiology, 9, 369-375, Scientific Publishers, Jodhpur, 2006. 9. Ahmad A, Andersen AS, Engvild KC. Rooting, growth and ethylene evolution of pea cuttings in response to chloroindole auxins. Physiol Plant 69: 137-140, 1987.
  • Ahmad A, Hayat S, Fariduddin Q et al. Germination and - amylase activity in the grains of wheat, treated with chloroindole acetic acids. Seed Technol 23: 88-91, 2001.
  • Ali B, Rani I, Hayat S et al. Effect of 4-Cl-indole-3-acetic acid on the seed germination of Cicer arietinum exposed to Cd. Acta Bot Croat 66: 57-65, 2007.
  • Ahmad A, Hayat S, Fariduddin Q, et al. Photosynthetic efficiency of plants of Brassica juncea, treated with chlorosubstituted auxins. Photosynthetica 39: 565-568, 2001.
  • Lindner RC. Rapid analytical method for some of the more common inorganic constituents of plant tissue. Plant Physiol 19: 76-89, 1944.
  • Fiske CH, Subba Row Y. The calorimetric determination of phosphorus. J Biol Chem 66: 373-400, 1925.
  • Yih RY, Clark HE. Total sugar and soluble protein content of boron deficient tomato root tips in relation to anatomy growth. Plant Physiol 40: 312-315, 1965.
  • Dubois M, Gills K, Hamilton JK et al. Calorimetric method for determination of sugars and related substances. Ann Chem 28: 350-356, 1956.
  • Lowry OH, Rosenbrough NJ, Farr AL et al. Soluble protein measurement with the Folin phenol reagent. J Biol Chem 193, 265-275, 1955.
  • Poschenreider C, Guinse B, Barcelo J. Influence of Cd on water relations, stomatal resistance, and abscisic acid content in expanding bean leaves. Plant Physiol 90: 1365-1371, 1989.
  • Stobart AK, Griffits W, Bukhari IA et al. The effect of Cd2+on the biosynthesis of chlorophyll in leaves of barley. Physiol Plant 63: 293-298, 1985.
  • Gadallah MAA. Interactive effect of heavy metals and temperature on the growth and chlorophyll, saccharides and soluble nitrogen contents in Phaseolus plants. Biol Plant 36: 373- 382, 1995.
  • Vassilev A, Berora M, Zlateve Z. Influence of Cd2+on growth chlorophyll content and water relations in young barley plants. Biol Plant 41: 601-606, 1998.
  • Hopkins WJ. Introduction to plant physiology, John Wiley & Sons Inc. New York, 1995.
  • Chugh LK, Gupta KV, Sawhney SK. Effects of Cd on enzymes of nitrogen metabolism in pea seedlings. Photochem 3: 395-400, 1992.
  • Gouia H, Ghorbel M, Meyer C. Effects of Cd on activity of nitrate reductase and on the other enzymes of nitrate assimilation pathway in bean. Plant Physiol Biochem 38: 629- 638, 2000.
  • Obata H, Inoue N, Umebayashi M. Effect of Cd on plasma membrane ATPase from plants roots differing in tolerance to Cd. Soil Sci Plant Nutr 42: 361-366, 1996.
  • Barcelo J, Poschenreider C. Plant water relations as effected by heavy metal stress: A review. J Plant Nutr 13, 1-37, 1990.
  • Heranandez LE, Carpena-Ruiz R, Garate A. Alterations in the mineral nutrition of pea seedlings exposed to Cd. J Plant Nutr 19: 1581-1598, 1996.
  • Hussain A. Response of Pisum sativum L. to exogenously applied plant growth regulators. PhD Thesis, Aligarh Muslim University, Aligarh, 2000.
  • Fariduddin Q, Ahmad A, Hayat S. Photosynthetic response of Vigna radiata to pre-sowing seed treatment with 28- Homobrassinolide. Photosynthetica 41: 307-310, 2003.
  • Mai YY, Lin JM, Zeng XL et al. Effect of homobrassinolide on the activity of nitrate reductase in rice seedlings. Plant Physiol Commun 2: 50-52, 1989.
  • Karcz W, Burdach Z. A comparison of the effect of IAA and 4- Cl-IAA on growth, proton secretion and membrane potential in maize coleoptiles segments. J Expt Bot 53: 1089-1093, 2002.
  • El-Shintinawy F, El-Ansary A. Differential effect of Cd2+and Ni2+on amino acid metabolism in soybean seedlings. Biol Plant 43: 79-84, 2000.
  • Ali B, Hayat S, Hasan SA et al. A comparative effect of IAA and 4-Cl-IAA on growth, nodulation and nitrogen fixation in Vigna radiata L. Wilczek. Acta Physiol Plant 30: 35-41, 2008.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

The effect of ascorbic acid and H2O2 treatment on the stability of anthocyanin pigments in berries

Elham NIKKHAH, - *, Masoud KHAIAMY, Reza HEIDARY, Ali Sorayaie AZAR

Genetic relationships of the genera Onobrychis, Hedysarum, and Sartoria using seed storage proteins

Emine ARSLAN*, Kuddisi ERTUĞRUL

Production of (S)-2'-fluorophenylethan-1-ol and (S)-3'- fluorophenylethan-1-ol by Alternaria alternata using ram horn peptone in microbial growth medium

Esabi Başaran KURBANOĞLU, - *, Namudar İzzet KURBANOĞLU, Kani ZILBEYAZ

Production of (S)-2'-fluorophenylethan-1-ol and (S)-3'- fluorophenylethan-1-ol by Alternaria alternata using ram horn peptone in microbial growth medium

Kani ZILBEYAZ, Namudar İzzet KURBANOĞLU, Esabi Başaran KURBANOĞLU

Stimulatory effects of ghrelin on spontaneous contractions in the rat myometrium

Alpaslan DAYANGAÇ, Selim KUTLU, Vahit KONAR

Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones

Manosh Kumar BISWAS, Uthpal Krishna ROY, Rafiul ISLAM, Monzur HOSSAIN

Nutritive value of wild edible and cultured mushrooms

Sevda KIRBAĞ, Mehmet AKYÜZ

Brown alga Sargassum muticum inhibits proinflammatory cytokines, iNOS, and COX-2 expression in macrophage RAW 264.7 cells

Brown alga Sargassum muticum inhibits Proinflammatory

Characterization of Essential Oils of Some Salvia Species and Their Antimycobacterial Activities

Characterization Of Essential Oils Of So ACTİVİTİES

The superiority of cv ‘rosea’ over cv ‘alba’ of periwinkle (Catharanthus roseus L.) in alkaloid production and other physiological attributes

Mohd IDREES, M. NAEEM*, M. Masroor A. KHAN