A Gene Expression Study of the TGF-b Signaling Pathway Components in Differentiating PC12 Cells

The neuronal differentiation process has been studied in great detail from many aspects. In the PC12 system, NGF was found to induce neuronal differentiation as measured by neurite extensions and neuronal marker expression. This effect was seen to be mainly conducted through the Ras/MAPK pathway; however, additional pathways are suspected to play a significant role in this cellular response. One such pathway is Smad signaling - NGF was previously reported to induce the expression of the TGF-b signal, although the physiological relevance of this was not completely understood. In this study, we analyzed the expression profiles of several TGF-b pathway components, in an attempt to understand the potential role of this pathway in NGF-induced differentiation of PC12 cells.

Anahtar Kelimeler:

PC12, TGF-b, Smad, NGF, gene expression profiling, neuronal differentiation

A Gene Expression Study of the TGF-b Signaling Pathway Components in Differentiating PC12 Cells

Keywords:

PC12, TGF-b, Smad, NGF, gene expression profiling, neuronal differentiation,

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Miyazawa K, Shinozaki M, Hara T,et al. Two major Smad pathways in TGF-βsuperfamily signaling. Genes to Cells 7: 1191–1204, 2002.
Massague J. TGF-beta signal transduction. Annu Rev Biochem 67: 753–791, 1998.
Mehler MF, Mabie PC, Zhang D, Kessler JA. Bone morphogenetic proteins in the nervous system. Trends Neurosci. 20: 309–317, 1997.
Chalazonitis A, Kalberg J, Twardzik DR, et al. Transforming growth factor beta has neurotrophic actions on sensory neurons in vitro and is synergistic with nerve growth factor. Dev. Biol. 152: 121–132, 1992.
Itoh S, Itoh F, Goumans M-J, ten Dijke P. Signaling of transforming growth factor-b family members through Smad proteins. Eur J Biochem 267: 6954–6967, 2000.
Miyazono K, Kusanagi K, Inoue H. Divergence and convergence of TGF-β/BMP signaling. J.Cell.Physiol. 187, 265–276, 2001.
Hayashi H, Ishisaki A, Imamura T. Smad mediates BMP-2-induced upregulation of FGF-evoked PC12 cell differentiation. FEBS Lett. 536, 30–34, 2003.
Hayashi H, Ishisaki A, Suzuki M, Imamura T. BMP-2 augments FGF-induced differentiation in PC12 cells through upregulation of FGF receptor-1 expression. J. Cell Sci. 114: 1387–1395, 2003.
Lutz M, Krieglstein K, Schmitt S, et al. Nerve growth factor mediates activation of the Smad pathway in PC12 cells. Eur. J. Biochem. 271: 920–931, 2004.