A gene expression study of the TGF-Beta signaling pathway components in differentiating

Sinir hücresi farklılaşması işlemi pek çok açıdan oldukça detaylı olarak çalışılmıştır. PCI2 sisteminde, NGF'in sinir hücresi farklılaşmasını indükiediği hem nörit uzantıları hem de nöronai işaret anlatımı ölçülmek suretiyle gösterilmiştir. Bu etkinin ana olarak Ras/MAPK yolağı tarafından ortaya çıkarıldığı bilinmekle birlikte, başka yolakların da bu hücresel tepkide büyük rol oynadığından şüphelenilmektedir. Bu yolaklardan bir tanesi Smad sinyallemesidir - NGF'in daha önce TGF-p sinyalinin anlatımına sebep olduğu gösterilmiştir ancak bunun fizyolojik önemi tam olarak anlaşılamamıştır. Bu çalışmada biz çeşitli TGF-p yolak elemanlarının anlatım profillerini inceleyerek bu yolağın NGF-indüklü PC12 farklılaşmasında olası işbirliğini anlamaya çalışmaktayız.

Farklılaşan PC12 hücrelerindeki TGF-Beta sinyalleme yolağı elemanlarına ait bir gen anlatımı çalışması

The neuronal differentiation process has been studied in great detail from many aspects. In the PC12 system, NGF was found to induce neuronal differentiation as measured by neurite extensions and neuronal marker expression. This effect was seen to be mainly conducted through the Ras/MAPK pathway; however, additional pathways are suspected to play a significant role in this cellular response. One such pathway is Smad signaling - NGF was previously reported to induce the expression of the TGF-p signal, although the physiological relevance of this was not completely understood. In this study, we analyzed the expression profiles of several TGF-p pathway components, in an attempt to understand the potential role of this pathway in NGF-induced differentiation of PCI 2 cells.

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