Shaheena AFROZ, Firoz MOHAMMAD, Shamsul HAYAT, Manzer H. SIDDIQUI

Exogenous Application of Gibberellic Acid Counteracts the Ill Effect of Sodium Chloride in Mustard

The response of mustard (Brassica juncea (L.) Czern & Coss cv. Varuna) to presowing seed treatment with sodium chloride (NaCl) was investigated. The plants raised from treated seeds were sprayed with water or 10-6 M GA3 at the 30- day stage. The seeds imbibed in 1 or 10 mM of NaCl resulted in a decrease in dry mass, leaf chlorophyll content, carbonic anhydrase activity (E.C. 4.2.1.1), nitrate reductase activity (E.C. 1.6.6.1) and net photosynthetic rate at the 60-day stage, and pod number and seed yield at harvest. However, spray application of GA3 neutralized the ill effect of soaking treatment in NaCl (1 or 10 mM).

Anahtar Kelimeler:

Brassica juncea, carbonic anhydrase, chlorophyll, nitrate reductase, salinity, seed yield Abbreviations: CA-carbonic anhydrase; DAS-days after sowing; DH- dry mass; NR-nitrate reductase; PN-net photosynthetic rate

Exogenous Application of Gibberellic Acid Counteracts the Ill Effect of Sodium Chloride in Mustard

Keywords:

Brassica juncea, carbonic anhydrase, chlorophyll, nitrate reductase, salinity, seed yield Abbreviations: CA-carbonic anhydrase; DAS-days after sowing; DH- dry mass; NR-nitrate reductase; PN-net photosynthetic rate,

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Khodary SEA. Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed maize plants. Int J Agri Biol 6: 5-8, 2004.
Ziska LH, Seeman LH, DeJong TM. Salinity induced limitations on photosynthesis in Prunus salicina, a decidous tree species. Plant Physiol 93: 864-870, 1990.
Dutt SK, Ball AR, Bandyopadhay AK. Salinity induced chemical changes in Casuarina equisetifolia Forst. Egyp J Soil Sci 31: 57- 63, 1991.
Kalir A, Poljakoff-Mayber A. Effect of salinity on respiratory pathways in root tips of Tamarix tetragyna. Plant Physiol 57: 167-170, 1976.
Crozier A, Turnball C. Gibberellins: Biochemistry and action in extension growth, What’s New. Plant Physiol 15: 9-12, 1984.
Arteca RN. Plant Growth Substances, CBS Publishers, New Delhi. 1997. pp 112-120.
Hayat S, Ahmad A, Mobin M et al. Carbonic anhydrase, photosynthesis and seed yield in mustard plants treated with phytohormones. Photosynthetica 39: 111-114, 2001.
Saxena OP, Pandey AV. Hormonal modulation for crop productivity of low quality seeds. In: Khan NA, Samiullah (ed.), Phytohormones in Crop Productivity under Different Environments, Scientific Publishers, Jodhpur, India, 2001, pp. 135-166.
Khan NA, Samiullah. Comparative effect of modes of gibberellic acid application on photosynthetic biomass distribution and productivity of rapeseed-mustard. Physiology & Molecular Biology of Plants 9: 141-145, 2003.
Angrish A, Kumar B, Datta KS. Effect of gibberellic acid and kinetin on nitrogen content and nitrate reductase activity in wheat under saline conditions. Ind J Plant Physiol 6: 172-177, 2001.
Aldesuquy HS, Ibrahim AH. Interactive effects of seawater and growth bioregulators on water relations abscisic acid concentration and yield of wheat plants. J Agro Crop Sci 187: 185-189, 2001
Chakrabarti N, Mukherji S. Effect of phytohormones pretreatment on metabolic changes in Vigna radiata under salt stress. J Env Biol 23: 295-300, 2002.
Mackinney G. Absorption of light by chlorophyll solutions. J Boil Chem 140: 315-322, 1941.
Dwivedi RS, Randhawa NS. Evaluation of a rapid test for the hidden hunger of Zinc in plants. Plant Soil 40: 445-451, 1974.
Jaworski EG. Nitrate reductase assay in intact plant tissue. Biochem Biophy Res Comm 43: 1274-1279, 1971.
Gomez KA, Gomez AA. Statistical procedure for agricultural research. Wiley International Publishers, New York, 1984. pp 156-174.
Wattana P, Monica AM. Water deficit on raffinose family oligosaccharides metabolism in coleus. Plant Physiol 121: 987- 993, 1999.
Kozlowski TT, Pallardy SG. Physiology of woody plants. Academic Press, San Diego, 1997.
Pezeshki SR, Chambers JL. Effect of soil salinity on stomatal conductance and photosynthesis of green ash (Fraxinus pennsylvanica). Can J Forest Res 16: 569-573, 1986.
Okabe K, Lindlar A, Tsuzuki M et al. Effects of carbinic anhydrase on ribulose 1, 5-bisphosphate carboxylase and oxygenase. FEBS Lett 114: 142-144, 1980.
Badger MR, Price GD. The role of carbonic anhydrase in photosynthesis. Ann Rev Plant Physiol Plant Mol Biol 45: 369- 392, 1994.
Khan NA, Ansari HR, Mobin M. Effect of gibberellic acid on carbonic anhydrase, photosynthesis, growth and yield of mustard. Biol Plant 38: 145-147, 1996.
Knypl JS, Krystyna M. Light and molybdenum requirements for substrate induction of nitrate reductase in cotyledons of Lactuca sativa. Biol Plant 21: 214-219, 1979.
Hayat S, Ahmad A. Soaking seeds of Lens culinaris with 28- homobrassinolide increased nitrate reductase activity and grain yield in the field in India. Ann Appl Biol 143: 121-124, 2003.
Dela-Rosa IM, Maiti RK. Biochemical mechanism in glossy sorghum lines for resistance to salinity stress. J Plant Physiol 146: 515-519, 1995.