Feruze ESER, Aka Hüsniye SAĞLIKER, Cengiz DARICI

The effects of giyphosate isopropylamine and trifluralin on the carbon mineralization of olive tree soils

Glyphosate isopropylamine ve trişuralin Türkiye’nin tarımsal alanlarında yaygın olarak kullanılan herbisitlerdir. Bu herbisitlerin önerilen tarla dozu (hem glyphosate hem de trişuralin için 480 g l-1) ve bu dozun 2 katı Akdeniz ikliminin etkisi altındaki Çukurova Üniversitesi kampus (Adana) alanında yetştirilen zeytin ağacı (Olea europaea L., Oleaceae) toprağına karbon mineralizasyon ölçümüyle toprak mikrobiyal aktivitesine etkilerini belirlemek amacıyla ilave edilmiştir. Tüm örneklerin C mineralizasyonu 30 gün boyunca (28 C, sabit nem) CO2 respirasyon yötemiyle belirlenmiştir. Glyphosate’in önerilen tarla dozu ve bu dozun 2 katı, herbisit içermeyen kontrolle kıyaslandığında C mineralizasyonu 30 günün sonunda anlamlı düzeyde artmıştır (P < 0.001). Fakat, trişuralinin önerilen tarla dozu ve bu dozun 2 katı kontrolden istatistiksel olarak farklı değildir (P > 0.05). Bu sonuçlara dayanarak toprak mikroorganizmalarının glyphosate’i karbon kaynağı olarak kullanma yeteneğine sahip olduğu sonucuna varmak mümkündür.

Zeytin topraklarının karbon mineralizasyonuna glyphosate isopropylamine ve trifluralin'in etkileri

Glyphosate isopropylamine and trifluralin are herbicides widely used in Turkish agriculture. The recommended field dose (RFD) (480 g of active ingredient l-1 for both glyphosate and trifluralin) and 2 x RFD of these herbicides were added to the soil of olive trees (Olea europaea L., Oleaceae) growing on the Çukurova University Campus (Adana) under Mediterranean climate conditions in order to determine their effects on soil microbial activity as measured by carbon mineralization. Carbon mineralization of all samples was determined by the CO2 respiration method over 30 days (28 °C, constant moisture). When the RFD and 2 x RFD of glyphosate were compared to a control treatment with no herbicide, they significantly stimulated carbon mineralization at the end of the 30 days (P < 0.001), but the RFD and 2 x RFD of trifluralin were not statistically different from the control (P > 0.05). Based on these results, it is possible to conclude that soil microorganisms are capable of using glyphosate as a carbon source.

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Araujo, A.S.F., R.T.R. Monteiro and R.B. Abarkeli. 2003. Effect of glyphosate on the microbial activity of two Brazilian soils. Chemosphere 52: 799-804.
Benlot, C. 1977. Recherches sur les Activites Biochimiques dans les Successions de sols Derives de Cendres Volcaniques sous Climat Tropical Humide (Zaire-Indonesie). ENS Lab., De Zoologie, Paris. Bouyoucos, G.S. 1951. A recalibration of the hydrometer for making mechanical analysis of soil. Agron. J. 43: 434-438.
Bukun, B. 2004. Critical periods for weed control in cotton in Turkey. Weed Res. 44: 404-412.
Busse M.D., A.W. Ratcliff, C.J. Shestak and R.F. Powers. 2001. Glyphosate toxicity and the effects of long-term vegetation control on soil microbial communities. Soil Biol. Biochem. 33: 1777-1789.
Camper, N.D., K. Stralka and H.D. Skipper. 1980. Aerobic and anaerobic degradation of profluralin and trifluralin. J. Environ. Sci. Heal. B 5: 457-473.
Demiralay, I. 1993: Toprak Fiziksel Analizleri. Atatürk Üniversitesi, Ziraat Fakültesi Yayınları, No: 143, Erzurum.
Duchaufour, P. 1970. Precis de Pedologie. Masson et C1e, Paris. Gomez, M.A. and M.A. Sagardoy. 1985. Influence of glyphosate herbicide on the microflora and mesofauna of a sandy soil in a semi-arid region. Rev. Latin Amer. Microbiol. 27: 351-357.
Haney, R.L., S.A. Senseman, F.M. Hons and D.A. Zuberer. 2000. Effect of glyphosate on soil microbial activity and biomass. Weed Sci. 48:89-93.
Haney, R.L., S.A. Senseman, L.J. Krutz and F.M. Hons. 2002a. Soil carbon and nitrogen mineralization as affected by atrazine and glyphosate. Biol. Fert. Soils 35: 35-40.
Haney, R.L., S.A. Senseman and F.M. Hons. 2002b. Effect of roundup ultra on microbial activity and biomass from selected soils. J. Environ. Qual. 31: 730-735.
Hang, M., C. Zhongyun, Z. Yuhua and C. Meichi. 2001. Effects of trifluralin on soil microbial populations and the nitrogen fixation activities. J. Environ. Sci. Heal. B 36: 569-579.
Jackson, M.L. 1958. Soil Chemical Analysis. Prentice-Hall, Inc., Englewood Cliffs, New York.
Johnston, W.H. and N.D. Camper. 1991. Microbial degradative activity in pesticide pretreated soil. J. Environ. Sci. Heal. B 26: 1-14.
Kleinbaum, D.G., L.L. Kupper, K.E. Muller and A. Nizam. 1998. Applied Regression Analysis and Other Multivariable Methods. Duxbury Press, California.
Malterre, F., G. Grebil, J.G. Pierre and M. Schiavon. 1997. Trifluralin behaviour in soil : a microlysimeter. Chemosphere 34: 447-454. Messersmith, C.G., O.C. Burnside and T.L. Lavy. 1971. Biological and non-biological dissipation of trifluralin from soil. Weed Sci. 19:285–290.
Meteoroloji Bülteni. 2001. Ortalama ve Ekstrem Kıymetler. Meteoroloji Müdürlüğü.Yayınları, Ankara.
Mueller, T.C., P.D. Mitchell, B.G. Young and A.S. Culpepper. 2005. Proactive versus reactive management of glyphosate-resistant or -tolerant weeds. Weed Technol. 19: 924-933.
Schaefer, R. 1967. Characteres et evolution des activites microbiennes dans une chaine de sols hidromorphes mesotrophiques de la plaine d'Alsace. Rev. Ecol. Biol. 4: 567-592.
Sprankle, P., W.F. Meggitt and D. Penner. 1975. Adsorption, mobility, and microbial degradation of glyphosate in the soil. Weed Sci. 23: 229-234.
Strynar, M., J. Dec, A. Benesi, A.D. Jones, R.A. Fry and J.M. Bollag. 2004. Using 19F NMR spectroscopy to determine trifluralin binding to soil. Environ. Sci. Technol. 38: 6645-6655.
Tiryaki, O., Ü. Yücel and G. Sezen. 2004. Biodegradation of trifluralin in Harran soil. J. Environ. Sci. Heal. B 39: 747-756.
Uludağ, A., I. Uremis, A.C. Ulger, B. Çakır and E. Aksoy. 2006. The use of maize as replacement crop in trifluralin treated cotton fields in Turkey. Crop Prot. 25: 275-280.
Vencill, W.K. 2002. Herbicide Handbook. WSSA, Lawrence, KS, USA.
Wardle, D.A. and D. Parkinson. 1990. Effects of three herbicides on soil microbial biomass and activity. Plant Soil 122: 21-28.
Zirai Mücadele Teknik Talimatları. 1995: Tarım ve Köy İşleri Bakanlığı Koruma ve Kontrol Genel Müdürlüğü Yayınları, No: 4, Ankara.