Shabana PARVEEN, Mahender Singh SAHARAN, Ajay VERMA, İndu SHARMA

Pathogenic variability among Tilletia indica isolates and distribution of heterothallic alleles in the Northwestern Plains Zone of India

Tilletia indica Mitra, the causal agent of Karnal bunt of wheat (KB), is a quarantined disease of international importance whose pathogen shows high variability due to its heterothallic nature. Pathogenic variation was studied under artificially inoculated conditions on 13 wheat genotypes including bread and dicoccum wheat. This study used 34 T. indica isolates collected from 6 major wheat growing states in India (Rajasthan, Haryana, Punjab, Himachal Pradesh, Uttarakhand, and Uttar Pradesh). Mean coefficient of infection of the isolates ranged from 2.11% (KBR4) to 14.60% (KBH1). Among the 13 genotypes screened, only 1 genotype was found to be highly susceptible to infection. KBR1 and KBHP3 were the most virulent and least aggressive isolates and had coefficients of infection of 79.42% and 6.22%, respectively, on the susceptible variety HD 2009. Pathogenic variability test revealed the existence of 3 different aggressive groups among the 34 selected isolates on a set of host differentials. A total of 64 monosporidial (Ms) lines were developed from the isolates, which originated from 6 states, representing 19 locations in the North Western plains zone of India. Self-paired Ms lines revealed 2 mating alleles. A total of 15 mating alleles were postulated using 46 Ms lines. This study confirmed the heterothallic nature of T. indica and the existence of multiple alleles controlling pathogenicity.

Anahtar Kelimeler:

Heterothallic allele, host differentials, Karnal bunt, Tilletia indica, wheat

Pathogenic variability among Tilletia indica isolates and distribution of heterothallic alleles in the Northwestern Plains Zone of India

Keywords:

Heterothallic allele, host differentials, Karnal bunt, Tilletia indica, wheat,

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Aujla SS, Gill KS, Sharma YR, Singh D, Nanda GS (1981). Effect of date of sowing and level of nitrogen on the incidence of Karnal bunt of wheat. Indian Ecol 8: 175–179.
Aujla SS, Grewal AS, Gill KS, Sharma I (1982). Artificial creation of Karnal bunt disease of wheat. Cereal Res Comm 10: 171–175.
Aujla SS, Sharma I (1990). Compatibility system in Neovossia indica. Indian Phytopath 43: 222–223.
Aujla SS, Sharma I, Singh BB (1987). Physiologic specialization of Karnal bunt of wheat. Indian Phytopath 40: 333–336.
Aujla SS, Sharma I, Singh BB (1989). Rating scale for identifying wheat varieties resistant to Neovossia indica (Mitra) Mundkar. Indian Phytopath 42: 161–162.
Bansal R, Singh DV, Joshi LM (1984). Comparative morphological studies in teliospores of Neovossia indica. Indian Phytopath 37: 355–357.
Bonde MR, Nester SE, Olsen MW, Berner DK (2004). Survival of teliospores of Tilletia indica in Arizona field soils. Plant Dis 88: 804–810.
Bonde MR, Peter G, Fluntes Davilla G, Aujla SS, Nanda GS, Philips JG (1996). Comparison of virulence of isolates of Tilletia indica, causal agent of Karnal bunt of wheat from India, Pakistan and Mexico. Plant Dis 80: 1071–1074.
Bonde MR, Peterson GL, Schaad NW, Smilanick JL (1997). Karnal bunt of wheat. Plant Dis 81: 1370–1377.
Chahal SS, Kaur S, Anita, Pannu PPS (2003). Compatibility in sporidia of Tilletia indica and Tilletia barclayana. Indian Phytopath 56: 78–81.
Duran R, Cromarty R (1977). Tilletia indica – A heterothallic wheat bunt fungus with multiple alleles controlling incompatibility. Phytopathology 67: 812–815.
FAO (1996). International Standards for Phytosanitary Measures: Guidelines for Pest Risk Analysis. Rome, Italy: Food and Agriculture Organization of the United Nations.
Fuentes-Dávila G (1984). Biology of Tilletia indica Mitra. MSc, Washington State University, Pullman WA, USA.
Goel LB, Singh DV, Srivastava KD, Joshi LM, Nagarajan S (1977). Smuts and bunts of wheat in India. New Delhi, India: Indian Agricultural Research Institute.
Hoffman JA (1982). Bunt of wheat. Plant Dis 66: 979–986.
Jones RD (2007). Arguments for a low risk of establishment of Karnal bunt disease of wheat in Europe. Eur J Plant Pathol 118: 93– 104.
Joshi LM, Singh DV, Srivatava KD, Wilcoxon RD (1983). Karnal bunt: a minor disease that is now a threat of wheat. Bot Rev 49: 309–330.
Krishna A, Singh RA (1983). Multiple alleles controlling the incompatibility in Neovossia indica. Indian Phytopath 36: 746–748.
Kumar J, Shoran J, Sharma I, Aggarwal R, Saharan MS, Sharma AK (2009). Distribution of heterothallic alleles of Tilletia indica in India. Indian Phytopath 62: 285–294.
Mitra M (1931). A new bunt on wheat in India. Ann Appl Biol 18: 178–179.
Mitra M (1935). Studies on stinking smut (bunt) of wheat with special reference to Tilletia indica Mitra. Indian J Agr Sci 5: 51–74.
Nagarajan S, Aujla SS, Nanda GS, Sharma I, Goel LB, Kumar J, Singh DV (1997). Karnal bunt (Tilletia indica) of wheat – a review. Rev Plant Pathol 76: 133–136.
Royer MH, Rytter JL (1985). Artificial inoculation of wheat with Tilletia indica from Mexico and India. Plant Dis 69: 317–319.
Sekhon KS, Saxena AK, Randhawa SK, Gill KS (1980). Effect of Karnal bunt disease on quality characteristics of wheat. Bull Grain Tech 18: 208.
Shoran J, editor (2006). Parentage Details. Progress Report 2005- 06, Crop Improvement Programme (Wheat and Triticale Varietal Trials and Wheat Physiology - Vol. 1). Karnal, India: Directorate of Wheat Research, Karnal.
Singh A (1994). Epidemiology and Management of Karnal Bunt Disease of Wheat. Pantnagar, India: University of Agriculture and Technology Research Bulletin No. 127.
Singh DV, Srivastava KD, Aggarwal R, Jain SK (1996). Factors associated with development and spread of Karnal bunt of wheat (Triticum aestivum) in north-western India. Indian J Agr Sci 66: 374–383.
Singh DV, Srivastava KD, Aggarwal R, Nagarajan S, Verma BR (1992). Mulching as a means of Karnal bunt management. Plant Dis Res 6: 115–116.
Smilanick JL, Hoffmann JA, Secrest LR, Wiese K (1988). Evaluation of chemical and physical treatments to prevent germination of Tilletia indica teliospores. Plant Dis 72: 46–51.
Tandon JP, Sethi AP (1991). Wheat Production Technology. Karnal, India: Karnal Directorate of Wheat Research.
Thirumalaisamy PP, Singh DV (2012). Variability of Indian isolates of Tilletia indica assessed by pathogenicity and molecular marker. J Phytopathol 160: 525–531.
Thirumalaisamy PP, Singh DV, Aggarwal R, Srivastava KD (2006). Pathogenic variability in Tilletia indica, the causal agent of Karnal bunt of wheat. Indian Phytopath 59: 22–26.
Warham EJ (1986). Karnal bunt disease of wheat: a literature review. Trop Pest Manage 32: 229–242.
Zadocks JC, Chang TT, Konzak CF (1974). A decimal code for the growth stage of cereals. Weed Res 14: 415–421.