Solvent optimization and characterization of fatty acid profile and antimicrobial and antioxidant activities of Turkish Pistacia terebinthus L. extracts

The present study investigated 12 different Pistacia terebinthus L. (terebinth) fruits, harvested or purchased in Turkey, for fatty acid composition, radical scavenging, antimicrobial activity, and total phenolic content. Solvent optimization for the samples was prepared by using a simplex lattice mixture design prior to the analysis. The optimum solvent mixture was 39% water and 61% acetone, based on the maximization of extraction yield of phenolic compounds. Total phenolic contents (TPCs) of the extracts varied from 12,189 mg GAE/1000 g extract to 36,392 mg GAE/1000 g extract. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities of the extracts varied over a wide range (8.86%-64.43%) and were highly correlated with TPCs. Agar diffusion was used to determine the antibacterial activity of the terebinth extracts against 2 gram-positive (Staphylococcus aureus and Listeria monocytogenes) and 2 gram-negative (Escherichia coli O157:H7 and Salmonella enterica subsp. enterica serovar Typhimurium) bacteria. L. monocytogenes and Salmonella Typhimurium were more susceptible to the extracts than E. coli O157:H7 and S. aureus. Considering the fatty acid composition of the extracts, palmitic acid, oleic acid, and linoleic acid were the dominant saturated, monounsaturated, and polyunsaturated fatty acids, respectively. In conclusion, this study confirmed the terebinth fruits' strong bioactive and antimicrobial properties and established their fatty acid profiles, indicating their potential for a variety of applications.

Anahtar Kelimeler:

Antimicrobial, antiradical, fatty acid, mixture design, phenolic, Pistacia terebinthus L.

Solvent optimization and characterization of fatty acid profile and antimicrobial and antioxidant activities of Turkish Pistacia terebinthus L. extracts

Keywords:

Antimicrobial, antiradical, fatty acid, mixture design, phenolic, Pistacia terebinthus L.,

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Alma MH, Nitz S, Kollmannsberger H, Digrak M, Efe FT, Yilmaz N (2004). Chemical composition and antimicrobial activity of the essential oils from the gum of Turkish pistachio (Pistacia vera L.). J Agr Food Chem 52: 3911–3914.
Al-Farsi MA, Lee CY (2008). Optimization of phenolics and dietary fibre extraction from date seeds. Food Chem 108: 977–985.
Anastasopoulos E, Kalogeropoulos N, Koliora AC, Kountouri A (2011). The influence of ripening and crop year on quality indices, polyphenols, terpenic acids, squalene, fatty acid profile, and sterols in virgin olive oil (Koroneiki cv.) produced by organic versus non-organic cultivation method. Int J Food Sci Technol 46: 170–178.
Atmani D, Chaher N, Berboucha M, Ayouni K, Lounis H, Boudaoud H, Debbache N, Atmani D (2009). Antioxidant capacity and phenol content of selected Algerian medicinal plants. Food Chem 112: 303–309.
Baytop T (1984). Therapy with medicinal plants in Turkey (Past and Present). Nobel Tıp Kitabevleri, İstanbul, Turkey.
Beltrán G, del Rio C, Sánchez S, Martinez L (2004). Influence of harvest date and crop yield on the fatty acid composition of virgin olive oils from cv. Picual. J Agr Food Chem 52: 3434–3440.
Benhammou B, Bekkara FA, Panovska TK (2008). Antioxidant and antimicrobial activities of the Pistacia lentiscus and Pistacia atlantica extracts. Afr J Pharm Pharmaco 2: 22–28.
Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 94: 223– 253.
Cam M, Aaby K (2010). Optimization of extraction of apple pomace phenolics with water by response surface methodology. J Agr Food Chem 58: 9103–9111.
Cattaneo P, Karman de Sutton G (1959). Composición química de aceites de olive argentinos. Rev. Argent. Grasas Aceites 1: 3–10 (in Spanish).
Costa AGV, Garcia-Diaz DF, Jimenez P, Silva PI (2013). Bioactive compounds and health benefits of exotic tropical red-black berries. J Funct Foods 5: 539–549.
Djenane D, Yanguela J, Montanes L, Djerbal M, Roncales P (2011). Antimicrobial activity of Pistacia lentiscus and Satureja montana essential oils against Listeria monocytogenes CECT 935 using laboratory media: efficacy and synergistic potential in minced beef. Food Contr 22: 1046–1053.
Dulger B, Gonuz A (2004). Antimicrobial activity of certain plants used in traditional Turkish medicine. Asian J Plant Sci 3: 104– 107.
Durmaz G, Gökmen V (2011). Changes in oxidative stability, antioxidant capacity, and phytochemical composition of Pistacia terebinthus oil with roasting. Food Chem 128: 410–414.
Farhat MB, Landoulsi A, Chaouch-Hamada R, Sotomayor JA, Jordán MJ (2013). Characterization and quantification of phenolic compounds and antioxidant properties of Salvia species growing in different habitats. Ind Crop Prod 49: 904–914.
García-Inza GP, Castro DN, Hall AJ, Rousseaux MC (2014). Responses to temperature of fruit dry weight, oil concentration, and oil fatty acid composition in olives (Olea europaea L. var. ‘Arauco’). Eur J Agron 54: 107–115.
Garcia-Salas P, Morales-Soto A, Segura-Carretero A, Fernandez- Gutierrez A (2010). Phenolic compound extraction systems for fruit and vegetable samples. Molecules 15: 8813–8826.
Giner-Larza, EM, Manez S, Giner-Pons RM, Recio MC, Rios J (2000). On the anti-inﬂammatory and anti-phospholipase A2 activity of extracts from lanostane-rich species. J Ethnopharmacol 73: 61–69.
Gogus F, Ozel MZ, Kocak D, Hamilton JF, Lewis AC (2011). Analysis of roasted and unroasted Pistacia terebinthus volatiles using direct thermal desorption-GCxGC-TOF/MS. Food Chem 129: 1258–1264.
Halliwell B, Gutteridge J, Cross C (1992). Free radicals, antioxidants, and human disease: how are we now? J Lab Clin Med 119: 598–620.
Henika RG (1982). Use of response surface methodology in sensory evaluation. Food Technol 36: 96–101.
Hwang D (2000). Fatty acids and immune responses—a new perspective in searching for clues to mechanism. Annu Rev Nutr 20: 431–456.
Jayaprakasha GK, Singh RP, Sakariah KK (2001). Antioxidant activities of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem 73: 285–290.
Kahkonen M, Hopia A, Heinonen M (2001). Berry phenolics and their antioxidant activity. J Agr Food Chem 49: 4076–4082.
Kallithraka S, Garcia-Viguera C, Bridle P, Bakker J (1995). Survey of solvents for the extraction of grape seed phenolics. Phytochem Analysıs 6: 265–267.
Kandhro A, Sherazi STH, Mahesar SA, Bhanger MI, Younis TM, Rauf A (2008). GC-MS quantification of fatty acid profile including trans FA in the locally manufactured margarines of Pakistan. Food Chem 109: 207–211.
Kavak DD, Altiok E, Bayraktar O, Ulku S (2010). Pistacia terebinthus extract: as a potential antioxidant, antimicrobial, and possible β-glucuronidase inhibitor. J Mol Catal B-Enzym 64: 167–171.
Kawashty SA, Mosharrafa SAM, El-Gibali M, Saleh NAM (2000). The flavonoids of four Pistacia species in Egypt. Biochem Syst Ecol 28: 915–917.
Khan MA, Baseer A (2000). Increased malondialdehyde levels in coronary heart disease. J Pak Med Assoc 50: 261–264.
Kordali S, Cakir A, Zengin H, Duru ME (2003). Antifungal activities of the leaves of three Pistacia species grown in Turkey. Fitoterapia 74: 164–167.
Kris-Etherton PM, Harris WS, Appel LJ (2002). Fish consumption, fish oil, omega-3 fatty acids, and cardiovascular disease. Circulation 106: 2747–2757.
Matthäus B, Özcan MM (2006). Quantitation of fatty acids, sterols, and tocopherols in turpentine (Pistacia terebinthus) growing wild in Turkey. J Agr Food Chem 54: 7667–7671.
Mori TA, Okamoto T, Aoyama M, Maruyama T, Niiya I, Yanagita T, Sugano M (2002). Trans fatty acids in margarines marketed in eleven countries. J Oleo Sci 51: 555–561.
Orhan IE, Senol FS, Gulpinar AR, Sekeroglu N, Kartal M, Sener B. (2012). Neuroprotective potential of some terebinth coffee brands and the unprocessed fruits of Pistacia terebinthus L. and their fatty and essential oil analyses. Food Chem 130: 882–888.
Ozkan G, Sagdic O, Baydar NG, Kurumahmutoglu Z. (2004). Antibacterial activities and total phenolic contents of grape pomace extracts. J Sci Food Agric 84: 1807–1811.
Ozturk I, Karaman S, Baslar M, Cam M, Caliskan O, Sagdic O, Yalcin H (2014). Aroma, sugar, and anthocyanin profile of fruit and seed of mahlab (Prunus mahaleb L.): optimization of bioactive compounds extraction by simplex lattice mixture design. Food Anal Method 7: 761–773.
Özcan C (2004). Characteristics of fruit and oil of terebinth (Pistacia terebinthus L) growing wild in Turkey. J Sci Food Agric 84: 517–520.
Poiana M, Mincione A (2004). Fatty acid evolution and composition of olive oils extracted from different olive cultivars grown in Calabrian area. Grasas Aceites 55: 282–290.
Rice-Evans C, Miller N, Paganga G (1997). Antioxidant properties of phenolic compounds. Trends Plant Sci 2: 152–159.
Rondanini DP, Castro DN, Searles PS, Rousseaux MC (2014). Constructing patterns of fatty acid composition and oil accumulation during fruit growth in several olive varieties and locations in a non-Mediterranean region. Eur J Agron 52: 237–246.
Sanz MJ, Terencio MC, Paya M (1993). Pharmacological actions of a new procyanidin polymer from Pistacia lentiscus L. Die Pharmazie 48: 152–153.
Sastre J, Pallardo FV, Vina J (2000). Mitochondrial oxidative stress plays a key role in aging and apoptosis. IUBMB Life 49: 427– 435.
Silici S, Sagdic O, Ekici L (2010). Total phenolic content, antiradical, antioxidant, and antimicrobial activities of Rhododendron honeys. Food Chem 121: 238–243.
Takabe W, Niki E, Uchida K, Yamada S, Satoh K, Noguchi N (2001). Oxidative stress promotes the development of transformation: involvement of a potent mutagenic lipid peroxidation product, acrolein. Carcinogenesis 22: 935–941.
Toker OS, Dogan M, Ersöz NB, Yilmaz MT (2013). Optimization of the content of 5-hydroxymethylfurfural (HMF) formed in some molasses types: HPLC-DAD analysis to determine effect of storage time and temperature levels. Ind Crop Prod 50: 137–144.
Topçu G, Ay M, Bilici A, Sarıürkcü C, Öztürk M, Ulubelen A (2007). A new flavone from antioxidant extracts of Pistacia terebinthus. Food Chem 103: 816–822.
Tornuk F, Karaman S, Ozturk I, Toker OS, Tastemur B, Sagdic O, Dogan M, Kayacier A (2013). Quality characterization of artisanal and retail Turkish blossom honeys: determination of physicochemical, microbiological, bioactive properties and aroma profile. Ind Crop Prod 46: 124–131.
Tous J, Romero A (1994). Cultivar and location effects on olive oil quality in Catalonia (Spain). Acta Hortic 356: 323–326.
Ulukanli Z, Karaborklu S, Ozturk B, Cenet M, Balcilar M (2012). Chemical composition, antibacterial, and insecticidal activities of the essential oil from the Pistacia terebinthus L. spp. Palaestina (Boiss) (Anacardiaceae). J Food Process Pres 38: 815–822.
Yalcin H, Toker OS, Dogan M (2012a). Effect of oil type and fatty acid composition on dynamic and steady shear rheology of vegetable oils. J Oleo Sci 61: 181–187.
Yalcin H, Toker OS, Ozturk I, Dogan M, Kisi O (2012b). Prediction of fatty acid composition of vegetable oils based on rheological measurements using nonlinear models. Eur J Lipid Sci Tech 114: 1217–1224.
Zhao X, Sun H, Hou A, Zhao Q, Wei T, Xin W (2005). Antioxidant properties of two gallotannins isolated from the leaves of Pistacia. BBA-Gen Subjects 1725: 103–110.