Lidia SUKOVATA, Tomasz JAWORSKI, Piotr KAROLEWSKI, Andrzej KOLK

The performance of Melolontha grubs on the roots of various plant species

Melolontha grubs are polyphagous and are adapted to feeding on plants of varying nutritional value. Our research sought to investigate whether host plant quality affects first-instar grub development, weight gain, or mortality. Ten plant taxa of the families Polygonaceae, Brassicaceae, Asteraceae, Fabaceae, and Pinaceae were tested. The quality of each plant species was estimated based on the root content of phenols, condensed tannins, soluble sugars, starch, nitrogen, and carbon. Retarded development, high mortality, and low weight gain were observed in grubs feeding on roots of Fagopyrum esculentum, Brassica rapa subsp. rapifera, and B. napus, whereas Tanacetum vulgare, Trifolium repens, and Lupinus polyphyllus roots proved to be the most beneficial for larval performance. Weight gain was positively correlated with the concentration of soluble sugars and starch in the plant roots. Starch was also positively correlated with the percentage of molted grubs. The revealed positive correlations may be explained by the fact that nonstructural sugars constitute an energy source that is essential for grub movement in the soil. Plant species that negatively affect cockchafer grubs may be used in integrated plant protection against these pests in agriculture, horticulture, and, to some extent, in forestry, e.g., in nurseries and postagricultural lands.

Anahtar Kelimeler:

Cockchafer, legumes, mustards, plant quality, Scots pine, tansy

The performance of Melolontha grubs on the roots of various plant species

Keywords:

Cockchafer, legumes, mustards, plant quality, Scots pine, tansy,

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Ahuja I, Rohloff J, Bones AM (2011). Defense mechanisms of Brassicaceae: implications for plant-insect interactions and potential for integrated pest management. In: Lichtfouse E, Hamelin M, Navarrete M, Debaeke P, editors. Sustainable Agriculture, Vol. 2. New York, NY, USA: Springer, pp. 623–670.
Arias-Cordero E, Ping L, Reichwald K, Delb H, Platzer M, Boland W (2012). Comparative evaluation of the gut microbiota associated with the below- and above-ground life stages (larvae and beetles) of the forest cockchafer, Melolontha hippocastani. PLoS ONE 7: e51557.
Askegaard M, Eriksen J (2007). Growth of legume and nonlegume catch crops and residual-N effects in spring barley on coarse sand. J Plant Nutr Soil Sci 170: 773–780.
Bauchop T, Clarke RTJ (1975). Gut microbiology and carbohydrate digestion in the larva of Costelytra zealandica (Coleoptera: Scarabaeidae). New Zeal J Zool 2: 237–243.
Benker U, Leuprecht B (2005). Field experience in the control of common cockchafer in the Bavarian region Spessart. IOBC/ WPRS Bull 28: 21–24.
De Geyeter E, Lambert E, Geelen D, Smagghe G (2007). Novel advances with plant saponins as natural insecticides to control pest insects. Pest Tech 1: 96–105.
Delb H, Mattes J (2001). Monitoring of Melolontha hippocastani F. at the upper Rhine Valley, Germany. J For Sci 47: 70–72.
Dolci P, Guglielmo F, Secchi F, Ozino OI (2006). Persistence and efficacy of Beauveria brongniartii strains applied as biocontrol agents against Melolontha melolontha in the Valley of Aosta (northwest Italy). J Appl Microbiol 100: 1063–1072.
Egert M, Stingl U, Bruun LD, Pommerenke B, Brune A, Friedrich MW (2005). Structure and topology of microbial communities in the major gut compartments of Melolontha melolontha larvae (Coleoptera: Scarabaeidae). Appl Environ Microb 71: 4556–4566.
Enkerli J, Widmer F, Keller S (2004). Long-term field persistence of Beauveria brongniartii strains applied as biocontrol agents against European cockchafer larvae in Switzerland. Biol Control 29: 115–123.
Erbaş Z, Gökçe C, Hazır S, Demirbağ Z, Demir İ (2014). Isolation and identification of entomopathogenic nematodes (Nematoda: Rhabditida) from the Eastern Black Sea region and their biocontrol potential against Melolontha melolontha (Coleoptera: Scarabaeidae) larvae. Turk J Agric For 38: 187–
Farrell JAK, Stufkens MW (1977). Resistance to the grass grub Costelytra zealandica (White) (Coleopter: Scarabaeidae) in some crop plant species. New Zeal J Agr Res 20: 503–505.
Farrell JAK, Sweney WJ (1972). Plants resistance to the grass grub, Costelytra zealandica (Col. Scarabaeidae). I. Resistance in pasture legumes. New Zeal J Agr Res 15: 904–908.
Farrell JAK, Sweney WJ (1974). Plant resistance to the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae). III. Resistance in Lotus and Lupinus. New Zeal J Agr Res 17: 69–72.
Feeny P (1970). Seasonal changes in oak leaf tannins and nutrients as a cause of spring feeding by winter moth caterpillars. Ecology 51: 565–581.
Fröschle M (1996). Methods to prevent and to control infestation of common cockchafer (Melolontha melolontha L.). IOBC/WPRS Bull 19: 104–108.
Grewal PS, Power KT, Grewal SK, Suggars A, Haupricht S (2004). Enhanced consistency in biological control of white grubs (Coleoptera: Scarabaeidae) with new strains of entomopathogenic nematodes. Biol Control 30: 73–82.
Haissig BE, Dickson R (1979). Starch measurement in plant tissue using enzymatic hydrolysis. Physiol Plantarum 47: 151–157.
Hansen J, Mİller I (1975). Percolation of starch and soluble carbohydrates from plant tissue for quantitative determination with anthrone. Anal Biochem 68: 87–94.
Hirsch AM (1992). Developmental biology of legume nodulation. New Phytol 122: 211–237.
Horber E, Wüst E (1958). Bekämpfung der Maikäferenerlinge (Melolontha vulgaris L.) mit der Zapfellengetriebenen Rotier Egge. Mitt Schweiz Landwirtsch 6: 33–43 (in German).
Hunter MD (2001). Out of sight, out of mind: the impacts of root- feeding insects in natural and managed systems. Agric For Entomol 3: 3–9.
Johnson G, Schaal LA (1957). Accumulation of phenolic substances and ascorbic acids in potato tuber tissue upon injury and their possible role in disease and resistance. Am Potato J 34: 200– 209.
Johnson SN, Gregory PJ (2006). Chemically-mediated host-plant location and selection by root-feeding insects. Physiol Entomol 31: 1–13.
Kain WM, Atkinson DS (1970). A rational approach to grass grub control. In: Matthews LG, editor. Proceedings of the 23rd New Zealand Weed and Pest Control Conference, 12–14 August 1970; Palmerston North, New Zealand. Auckland, New Zealand: New Zealand Weed and Pest Control Society, pp. 180–183.
Karolewski P, Zadworny M, Mucha J, Napierała-Filipiak A, Oleksyn J (2010). Link between defoliation and root vitality in five understory shrubs with different resistance to insect herbivores. Tree Physiol 30: 969–978.
Keller S, Schweizer C, Shah P (1999). Differential susceptibility of two Melolontha populations to infections by the fungus Beauveria brongniartii. Biocontrol Sci Techn 9: 441–446.
Keller S, Zimmermann G (2005). Scarabs and other soil pests in Europe: situation, perspectives and control strategies. IOBC/ WPRS Bull 28: 9–12.
Kirkegaard JA, Sarwar M (1998). Biofumigation potential of brassicas. I. Variation in glucosinolate profiles of diverse field- grown brassicas. Plant Soil 201: 71–89.
Koppenhöfer AM, Wilson M, Brown I, Kaya HK, Gaugler R (2000). Biological control agents for white grubs (Coleoptera: Scarabaeidae) in anticipation of the establishment of the Japanese beetle in California. J Econ Entomol 93: 71–80.
Krell FT (2004). Identification of larvae and adults of the central European Melolontha species (Coleoptera: Scarabaeoidea). Laimburg J 1: 211–219 (in German with an abstract in English).
Kumar K, Goh KM (2002). Management practices of antecedent leguminous and non-leguminous crop residues in relation to winter wheat yields, nitrogen uptake, soil nitrogen mineralization and simple nitrogen balance. Eur J Agron 16: 295–308.
Lattanzio V, Lattanzio VMT, Cardinali A (2006). Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. In: Imperato F, editor. Phytochemistry: Advances in Research. 1st ed. Kerala, India: Research Signpost, pp. 23–67.
Malinowski H (2007). Current problems of forest protection connected with the control of cockchafers (Melolontha spp.). Prog Plant Prot/Post Ochr Roślin 47: 314–322 (in Polish with an abstract in English).
Malinowski H (2009). Possibility of forest protection against insects damaging root systems with the use of prophylactic, mechanical and agrotechnical methods. Sylwan 153: 723–732 (in Polish with an abstract in English).
Malinowski H, Augustyniak A, Łabanowska BH (2001). New possibilities of the reduction insect populations feeding on plant roots. Prog Plant Prot/Post Ochr Roślin 41: 175–181 (in Polish with an abstract in English).
Price ML, Van Scoyoc S, Butler LG (1978). A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain. J Agr Food Chem 26: 1214–1218.
Rewieński S (1887). Teka oszczędnych wskazówek. Warsaw, Poland: Nakład Maurycego Orgelbranda (in Polish).
Rossiter MC, Schultz JC, Baldwin IT (1988). Relationships among defoliation, red oak phenolics, and gypsy moth growth and reproduction. Ecology 69: 267–277.
Rożyński F (1926). W sprawie walki z chrząszczem majowym (Melolontha vulgaris). Prz Leśn 7: 32–38 (in Polish).
Sierpiński Z (1975). Ważniejsze owady – szkodniki korzeni drzew i krzewów leśnych. Warsaw, Poland: PWRiL (in Polish).
Singleton VI, Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagent. Am J Enol Viticult 16: 144–158.
Sokal RR, Rohlf FJ (1995). Biometry: Principles and Practice of Statistics in Biological Research. 3rd ed. New York, NY, USA: W.H. Freeman.
Stocki J, Malinowski H (2000). Integrated method of the control of Melolontha spp. larvae and adults in Poland. IOBC/WPRS Bull 19: 69–73.
Sutherland ORW (1971). Feeding behaviour of the grass grub Costelytra zealandica (White) (Coleoptera: Melolonthinae) 1. The influence of carbohydrates. New Zeal J Sci 14: 18–24.
Sutherland ORW, Hillier JR (1974). Feeding behaviour of the grass grub Costelytra zealandica (White) (Coleoptera: Melolonthinae) 3. The influence of amino acids, ascorbic acid, and inorganic salts. New Zeal J Zool 1: 211–216.
Sutherland ORW, Hood ND, Hillier JR (1975). Lucerne root saponins a feeding deterrent for the grass grub, Costelytra zealandica (Coleoptera: Scarabaeidae). New Zeal J Zool 2: 93–100.
Sutherland ORW, Hutchins RFN, Greenfield WJ (1982). Effect of lucerne saponins and Lotus condensed tannins on survival of grass grub, Costelytra zealandica. New Zeal J Zool 9: 511–514.
Śliwa E (1993). Szkodniki korzeni drzew i krzewów. Warsaw, Poland: Wydawnictwo Świat (in Polish).
Švestka M (2006). Distribution of tribes of cockchafers of the genus Melolontha in forests of the Czech Republic and the dependence of their swarming on temperature. J For Sci 52: 520–530.
Tol RWHM, Wiegers GL, Kogel WJ (2011). Attraction and repellence of grubs in soil systems as part of pest control strategies: the cockchafer Melolontha melolontha example. In: Structure, Function and Dynamics of Biomolecules Used in Pest Management of Horticultural Crops. Odisha, India: Regional Centre, Central Tuber Crops Research Institute, pp. 227–230.
Tóth J (1998). Damage caused by cockchafers (Melolontha spp.) in Hungary during the last 30 years. In: McManus ML, Liebhold AM, editors. Proceedings; Population Dynamics, Impacts, and Integrated Management of Forest Defoliating Insects, 18–23 August 1996; Banska Stiavnica, Slovakia. Radnor, PA, USA: USDA Forest Service, p. 341.
Voroncov AI (1982). Lesnaia Entomologiia. Moscow, USSR: Vysshaia Shkola (in Russian).
Wagner SC (2011). Biological nitrogen fixation. Nature Education Knowledge 3: 15.
Wilson E (1978). White clover and grass grub. I. Screening technique for resistance and tolerance. New Zeal J Agr Res 21: 715–721.