Virginia SARROPOULOU, Kortessa DIMASSI-THERIOU, İoannis THERIOS

L-arginine impact on cherry rootstock rooting and biochemical characteristics in tissue culture

In the present study, the effects of indole-3-butyric acid (IBA) separately and simultaneously with L-arginine on the morphogenic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens) were investigated. In the CAB-6P rootstock, the best root number and root length results were obtained with 2 mg L1 IBA plus 0.5 mg L1 L-arginine and 1 mg L1 IBA plus 1 mg L1 L-arginine, respectively. The rooting percentage was highest (100%) with 2 mg L1 IBA alone or combined with 1 mg L1 L-arginine. In the Gisela 6 explants, 2 mg L1 IBA without L-arginine significantly enhanced root number (9.27) and root fresh and dry weight, as well as rooting percentage (100%). Root length was longest (38 mm) in the combination of the lowest IBA (0.5 mg L1) and highest L-arginine (2 mg L1) concentrations. Thus, L-arginine promotes the positive effect of IBA on rooting with regard to both root number and root length in both cherry rootstocks. In the CAB-6P explants, L-arginine combined with IBA had an inhibitory effect on leaf chlorophyll content, whereas in the Gisela 6 rootstock it had absolutely no effect. In the CAB-6P rootstock, depleted levels of proline in roots were observed, showing the osmoregulation and osmotic adjustment mechanisms. The carbohydrate concentration in leaves was greatest with 0.5 mg L1 IBA plus 0.5 mg L1 L-arginine, and in roots with 2 mg L1 IBA plus 0.5 mg L1 L-arginine. In the Gisela 6 explants, IBA (1 and 2 mg L1) applied both alone and along with L-arginine, as well as the combination of the lowest IBA (0.5 mg L1) and the highest L-arginine (2 mg L1) concentrations, considerably increased leaf carbohydrate concentration compared to the control. In roots, carbohydrate concentration was maximum with 2 mg L1 IBA plus 0.5 mg L1 L-arginine. The elevated levels of proline in roots with 1 mg L1 IBA plus 0.5 mg L1 L-arginine indicate stressful conditions.

Anahtar Kelimeler:

Amino acids, auxins, carbohydrates, plant tissue culture, proline, rooting

L-arginine impact on cherry rootstock rooting and biochemical characteristics in tissue culture

Keywords:

Amino acids, auxins, carbohydrates, plant tissue culture, proline, rooting,

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Abd El-Monem AA (2007). Polyamines as modulators of wheat growth, metabolism and reproductive development under high temperature stress. PhD, Ain Shamas University, Cairo, Egypt.
Abdul Qados AMS (2010). Effect of arginine on growth, nutrient composition, yield and nutritional value of mung bean plants grown under salinity stress. Nature Sci 8: 30–42.
Abdul Qados AMS (2009). Effect of arginine on growth, yield and chemical constituents of wheat grown under salinity condition. Acad J Plant Sci 2: 267–278.
Agulló-Antón MA, Sánchez-Bravo J, Acosta M, Druege U (2008). Relation between light conditions and carbohydrate levels during the storage of carnation cuttings: effects on adventitious roots formation. In: Proceedings of the 5th International Symposium on Adventitious Root Formation: From Cell Fate Flexibility to Root Meristem Determination and Biomass Formation. Poster 1, pp. 115–116.
Ahkami AH, Melzer M, Haensch KT, Franken P, Hause B, Druege U, Hajirezaei MR (2008). Possible involvement of carbohydrate metabolism in adventitious root formation in Petunia hybrida cuttings. In: Proceedings of the 5th International Symposium on Adventitious Root Formation: From Cell Fate Flexibility to Root Meristem Determination and Biomass Formation, Abstracts Book, p. 81.
Alia A, Saradhi PP (1993). Suppression in mitochondrial electron transport is the prime cause behind stress induced proline accumulation. Biochem Biophys Res Commun 193: 54–58.
Alvarez R, Nissen SJ, Sutter EG (1989). Relationship between indole- 3-acetic acid levels in apple (Malus pumila Mill) rootstocks cultured in vitro and adventitious root formation in the presence of indole-3-butyric acid. Plant Physiol 89: 439–443.
Anjum F, Rishi V, Ahmad F (2000). Compatibility of osmolytes with Gibbs energy of stabilization of proteins. Biochim Biophys Acta 1476: 75–84.
Baraldi R, Marino G, Rosati P, Golinelli A (1988). Sour cherry (Prunus cerasus) micropropagation. Acta Hortic 227: 405–407.
Camara TR, Willadino L, Torne JM, Rodriguez P, Santos MA (1998). Effect of putrescine and salt stress on maize callus. Rev Bras Fisiol Veg 10: 153–156.
Cantón FR, Suárez MF, Cánovas FM (2005). Molecular aspects of nitrogen mobilization and recycling in trees. Photosynth Res 83: 265–278.
Chen CT, Kao CH (1993). Osmotic stress and water stress have opposite effects on putrescine and proline production in excised rice leaves. Plant Growth Regul 13: 197–202.
Chen H, McCaig BC, Melotto M, He SY, Howe GA (2004). Regulation of plant arginase by wounding, jasmonate, and the phytotoxin coronatine. J Biol Chem 279: 45998–46007.
Chriqui D, D’Orazi D, Bagni N (1986). Ornithine and arginine decarboxylases and polyamine involvement during in vivo differentiation and in vitro dedifferentiation of Datura innoxia leaf explants. Physiol Plant 68: 589–596.
Chriqui D (1985). Induction de proliferation des cellules prerhizogenes: auxines et polyamines. Bull Soc Bot Fr Actual Bot 132: 127–141 (in French).
Couée I, Hummel I, Sulmon C, Gouesbet G, El-Amrani A (2004). Involvement of polyamines in root development. Plant Cell Org Tiss Cult 76: 1–10.
Delgado E, Mitchell RAC, Parry MAJ, Driscoll SP, Mitchell VJ, Lawlor DW (1994). Interacting effects of CO2 concentration, temperature and nitrogen supply on the photosynthesis and composition of winter wheat leaves. Plant Cell Environ 17: 1205–1213.
Dimassi-Theriou K, Therios I (2006). General Pomology – Part A: Propagation and Rootstocks of Fruit Trees. Thessaloniki, Greece: Gartaganis (in Greek).
El-Bassiouny HMS, Mostafa HA, El-Khawas SA, Hassanein RA, Khalil SI, Abd El-Monem AA (2008). Physiological responses of wheat plant to foliar treatments with arginine or putrescine. Aust J Basic Appl Sci 2: 1390–1403.
El-Shraiy AM, Hegazi AM (2009). Effect of acetylsalicylic acid, indole-3-butyric acid and gibberellic acid on plant growth and yield of pea (Pisum sativum L.). Aust J Basic Appl Sci 3: 3514–3523.
Epstein E, Lavee S (1984). Conversion of indole-3-butyric acid by cuttings of grapevine (Vitis vinifera L.) and olive (Olea europaea L.). Plant Cell Physiol 25: 697–703.
Gao HJ, Yang HQ, Wang JX (2009). Arginine metabolism in roots and leaves of apple (Malus domestica Borkh.): the tissue- specific formation of both nitric oxide and polyamines. Sci Hortic 119: 147–152.
González A, Rodríguez R, Tamés RS (1991). Ethylene and in vitro rooting of hazelnut (Corylus avellana) cotyledons. Physiol Plant 81: 227–233.
Hasnat R, Abbasi NA, Ahmad T, Hafiz IA (2007). Induction and regeneration of hypocotyl derived calli in hot chilli (Capsicum frutescens L.) varieties. Pak J Bot 39: 1787–1795.
Hassanein RA, Khalil SI, El-Bassiouny HMS, Mostafa HAM, El- Khawas SA, Abd El-Monem AA (2008). Protective role of exogenous arginine or putrescine treatments on heat shocked wheat plant. In: Proceedings of the 1st International Conference on Biological and Environmental Sciences, 13–16 March 2008; Hurghada, Egypt.
Haq IU, Soomro F, Parveen N, Dahot MU, Mirbahar AA (2011). Certain growth related attributes of micropropagated banana under different salinity levels. Pak J Bot 43: 1655–1658.
Hartmann HT, Kester DE, Davies FT, Geneve L (1997). The biology of propagation by cuttings. In: Hartmann HT, Kester DE, Davies FT, Geneve L, editors. Plant Propagation: Principles and Practices. Upper Saddle River, NJ, USA: Prentice Hall International, pp. 276–312.
Heath RL, Packer L (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125: 189–198.
Indrayanto G, Erawati T, Sandosa MH (1995). Effect of L-arginine, casein hydrolysate, banana powder and sucrose on growth and solasodine production in shoot cultures of Solanum laciniatum. Plant Cell Tiss Org Cult 43: 237–240.
Jabeen F, Shahbaz M, Ashraf M (2008). Discriminating some prospective cultivars of maize (Zea mays L.) for drought tolerance using gas exchange characteristics and proline contents as physiological markers. Pak J Bot 40: 2329–2343.
James DJ (1983a). Adventitious root formation ‘in vitro’ in apple rootstocks (Malus pumila) I. Factors affecting the length of the auxin-sensitive phase in M.9. Physiol Plant 57: 149–153.
James DJ (1983b). Adventitious root formation ‘in vitro’ in apple rootstocks (Malus pumila) II. Uptake and distribution of indole-3-acetic acid during the auxin sensitive phase in M.9 and M.26. Physiol Plant 57: 154–158.
James DJ, Thurnbon IJ (1981a). Shoot and root initiation in vitro in the apple rootstock M.9 and the promotive effects of phloroglucinol. J Hortic Sci 56: 15–20.
James DJ, Thurnbon IJ (1981b). Phenolic compounds and other factors controlling rhizogenesis in vitro in the apple rootstocks M.9 and M.26. Z Pflanzenphysiol 105: 11–20.
Kamada Η, Harada Η (1979). Influence of several growth regulators and amino acids on in vitro organogenesis of Torenia fournieri Lind. J Exp Bot 30: 27–36.
Kesba HH (2005). Effect of amino acids foliar application on Meloidogyne incognita and biochemical alterations in grape roots. Bull Fac Agric Cairo Univ Egypt 56: 617–630.
Khan MA, Gul B, Weber DJ (2000). Germination responses of Salicornia rubra to temperature and salinity. J Arid Environ 45: 207–214.
Le CL (1985). Influence of temperature on in vitro root initiation and development of apple rootstock M26. HortScience 20: 451–452.
Litwińczuk W (2002). Propagation of black chokeberry (Aronia melanocarpa Elliot) through in vitro culture. Electronic Journal of Polish Agricultural Universities 5: 06.
Machakova I, Zazimalova E, George EF (2008). Plant growth regulators I: Introduction; auxins, their analogues and inhibitors. In: George EF, Hall MA, De Klerk GJ, editors. Plant Propagation by Tissue Culture. Dordrecht, the Netherlands: Springer, pp. 175–204.
Mashayekhi-Nezamabadi K (2000). The protein synthesis spectrum during the induction phase of somatic embryogenesis in carrot (Daucus carota L.) cultures and the role of nitrogen forms for embryo development. PhD, Justus Liebig University, Giessen, Germany.
Mostafa HAM, Hassanein RA, Khalil SI, El-Khawas SA, El- Bassiouny HMS, Abd El-Monem AA (2010). Effect of arginine or putrescine on growth, yield and yield components of late sowing wheat. J Appl Sci Res 6: 177–183.
Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.
Orlikowska T (1992). Influence of arginine on in vitro rooting of dwarf apple rootstock. Plant Cell Tiss Org Cult 31: 9–14.
Paschalidis AK, Roubelakis-Angelakis KA (2005). Sites and regulation of polyamine catabolism in the tobacco plant. Correlations with cell division/expansion, cell cycle progression and vascular development. Plant Physiol 38: 2174–2184.
Plummer DT (1987). An Introduction to Practical Biochemistry. 3rd ed. London, UK: McGraw Hill.
Saradhi PP, Alia A, Arosa S, Prasad KVSK (1995). Proline accumulates in plants exposed to UV radiation and protects them against UV induced peroxidation. Biochem Biophys Res Commun 209: 1–5.
Smirnoff N, Cumbes QJ (1989). Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28: 1057–1060.
Theodoropoulos PA, Roubelakis-Angelakis KA (1989). Mechanism of arginine transport in Vitis vinifera L. protoplasts. J Exp Bot 40: 1223–1230.
Thorpe TA (1993). In vitro organogenesis and somatic embryogenesis: physiological and biochemical aspects. In: Roubelakis- Angelakis T, Tran Van Thanh K, editors. Morphogenesis in Plants. New York, NY, USA: Plenum Press, pp. 19–38.
Troll W, Lindsley J (1955). A photometric method for the determination of proline. J Biol Chem 215: 655–660.
Vasudevan A, Selvaraj N, Ganapathi A, Kasthurirengan S, Ramesh Anbazhagan V, Manickavasagam M (2004). Glutamine: a suitable nitrogen source for enhanced shoot multiplication in Cucumis sativus L. Biol Plant 48: 125–128.
Venekamp JH (1989). Regulation of cytosolic acidity in plants under conditions of drought. Physiol Plant 76: 112–117.
Wei H, Ca Q, Rahn R, Zhang X, Wang Y, Lebwohl M (1998). DNA structural integrity and base composition affect ultraviolet light-induced oxidative DNA damage. Biochemistry 37: 6485– 6490.
Wintermans JFGM, De Mots A (1965). Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol. Biochim Biophys Acta 109: 448–453.
Yagi MI, Al-Abdulkareem SS (2006). Effects of exogenous arginine and uric acid on Eruca sativa Mill shoots grown under saline conditions. J Sc Tech 7: 1–11.
Zulfiqar B, Abbasi NA, Ahmad T, Hafiz IA (2009). Effect of explant sources and different concentrations of plant growth regulators on in vitro shoot proliferation and rooting of avocado (Persea americana Mill.) cv. “Fuerte”. Pak J Bot 41: 2333–2346.