Evaluation of yield and quality parameters of phosphorous-solubilizing and N-fixing bacteria inoculated in wheat (Triticum aestivum L.)

Considering current agricultural practices and certain indicators, it is impossible to comment on sustainability of these practices. Chemical fertilizers are among such unsustainable practices. Because of increasing mineral fertilizer costs and negative environmental impacts of these fertilizers, the interest in biological nitrogen fixation is increasing within the scope of sustainable agriculture (without significant yield losses). Therefore, effects of phosphorus-solubilizing (Bacillus megatherium var. phosphaticum [M-13]) and nitrogen-fixing (Stenotrophomonas maltophilia [82] and Ralstonia pickettii [73]) bacteria and chemical fertilizer treatments on wheat yield and quality parameters were compared with control treatment in present study. Significant differences were observed among treatments with regard to entire parameters. Although the best results were observed in chemical fertilizer treatments, single, dual, and triple bacteria combinations yielded significant increases in grain filling period, number of spikes per square meter, number of kernels per spike, 1000-kernel weight, biological yield, grain yield, grain protein ratio, plant protein ratios in flowering, and physiological maturity periods. Based on these findings and considering the M-13 + 73 + 82 triple bacteria combination with regard to grain yield and protein ratios, it may be concluded that such treatments may reduce the amount of fertilizer to be used by 20%. Among the dual bacteria treatments, 73 + 82 and 82 + M-13 were found to be advantageous with regard to grain filling period, 73 + M-13 with regard to 1000-kernel weight and grain protein ratio, and M-13 + 73 and M-13 + 82 with regard to plant protein ratio in the physiological maturity period.

Anahtar Kelimeler:

Key words: Inoculation, rhizobacteria, yield response, wheat

Evaluation of yield and quality parameters of phosphorous-solubilizing and N-fixing bacteria inoculated in wheat (Triticum aestivum L.)

Keywords:

Key words: Inoculation, rhizobacteria, yield response, wheat,

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Abd-Alla MH (1994). Use of organic phosphorus by Rhizobium leguminosarum biovar viceae phosphatases. Biol Fertil Soils 18: 216–218. Amalraj ELD, Maiyappan S, Peter AJ (2012). In vivo and in vitro studies of Bacillus megaterium var. phosphaticum on nutrient mobilization, antagonism and plant growth promoting traits. J Ecobiotech 4: 35–42.
American Association of Cereal Chemists (1983). Approved Methods of the AACC, 8th ed. Method 46-12A, approved May 1969, revised October 1984. St Paul, MN, USA: AOAC.
Asea PEA, Kucey RMN, Stewart JWB (1988). Inorganic phosphate solubilization by two Penicillium species in solution culture and soil. Soil Bid Biochem 20: 459–464.
Babaogli F, Khoeai FR, Mehrdad Y (2012). Effect of biological fertilizer on yield and yield components of corn (Zea mays) cv. S.C. 504 in drought condition. J Appl Environ Biol Sci 2: 117–122.
Bahrani A, Pourreza J, Joo MH (2010). Response of winter wheat to co-inoculation with Azotobacter and arbescular mycorrhizal fungi (AMF) under different sources of nitrogen fertilizer. J Agric Environ Sci 8: 95–103.
Banik S, Ninawe A (1988). Phosphate solubilising microorganism in water and sediments of a tropical estuary and the adjacent coastal Arabian Sea, in relation to their physicochemical properties. J Indian Soc Coast Agric Res 6: 75–83.
Belimov AA, Kojemiakov PA, Chuvarliyeva CV (1995). Interaction between barley and mixed cultures of nitrogen fixing and phosphate solubilizing bacteria. Plant Soil 173: 29–37.
Biswas JC, Ladha JK, Dazzo FB (2000). Rhizobia inoculation improves nutrient uptake and growth of lowland rice. Soil Sci Soc Am J 64: 1644–1650.
Broadbent FE, Nakashima T (1968). Plant uptake and residual value of six tagged nitrogen fertilizers. Soil Sci Soc Am J 55: 130–135.
Bulut S, Öztürk A, Karaoğlu MM, Yıldız N (2013). Effects of organic manures and non-chemical weed control on wheat. II. Grain quality. Turk J Agric For 37: 271–280.
Çakmakçı R, Dönmez MF, Erdogan Ü (2007). The effect of plant growth promoting rhizobacteria on barley seedling growth, nutrient uptake, some soil properties, and bacterial counts. Turk J Agric For 31: 189–199.
Çakmakçı R, Kantar F, Algur ÖF (1999). Sugar beet and barley yield in relation to Bacillus polymxa and Bacillus megaterium var. Phosphaticum inoculation. J Plant Nutr Soil Sci 62: 437–442.
Daneshmand NG, Bakhshandeh A, Rotsam MR (2012). Bio fertilizer affects yield and yield components of wheat. Int J Agric Res Rev 2: 699–704.
Dhale DA, Chatte SN, Jadhav VT (2011). Response of bioinoculants on growth, yield and fiber quality of cotton under irrigation. Agric Biol J N Am 2: 376–386.
Diaz-Zorita M, Fernandez-Canigia MV (2008). Field performance of a liquid formulation of Azospirillum brasilense on dryland wheat productivity. Eur J Soil Biol 45: 3–11.
Frederik JR, Camberato JJ (1995). Water and nitrogen effects on winter wheat in the Southeastern Coastal Plain: I. Grain yield and kernel traits. Agron J 87: 521–526.
Gauer LE, Grant CA, Gehl DT, Bailey LD (1992). Effects of nitrogen fertilization on grain protein content, nitrogen uptake and nitrogen use efficiency of spring wheat (Triticum aestivum L.) cultivars in relation to estimated moisture supply. Can J Plant Sci 72: 235–241.
Hasanpour J, Panahi M, Sadeghi Pour Marvi M, Arabsalmani K (2012). Effect of inoculation with VA mycorrhiza and azotobacter on grain yield, LAI and protein of wheat on drought stress condition. Int J Agric Sci 2: 466–476.
Hedge DM, Dwivedi BS, Bahu Sudhakara SN (1999). Biofertilizers for cereal production in India. Indian J Agric Sci 69: 73–83.
Hu X, Boyer GL (1996). Siderophore-mediated aluminium uptake by Bacillus megatherium ATCC 19213. Appl Environ Microb 62: 4044–4048.
Illmer P, Barbato A, Schinner F (1995). Solubilization of hardly soluble AlPO 4 with P–-solubilizing microorganisms. Soil Biol Biochem 27: 260–270.
Ito T (1993). Enzymatic determination of itoic acid, a Bacillus subtilis siderophore and 2,3-dihydroxybenzoic acid. Appl Environ Microb 59: 2343–2345.
Jha D, Mathur RS (1993). Combined effect of nitrogenous fertilizers and Azospirillum brasilense on the yield of nitrogen uptake by pearl millet. Int J Trop Agric 11: 31–35.
Kantar F (1997). Prospect of bio-organic fertilizers and sustainable agriculture in Turkey. In: Proceedings of the Training Course on Bio-organic Farming Systems for Sustainable Agriculture (1995, Cairo, Egypt), pp. 263–276.
Khorshidi YR, Ardakani MR (2011). Response of yield and yield components of rice (Oryza sativa L.) to Pseudomonas fluorescens and Azospirillum lipoferum under different nitrogen levels. J Agric Environ Sci 10: 387–395.
Kiani MJ, Abbasi MK, Rahim N (2005). Use of organic manure with mineral N fertilizer increases wheat yield at Rawalakot Azad Jammu and Kashmir. Agron Soil Sci 51: 299–309.
Kim T, Jung W, Lee B, Yoneyama T, Kim H, Kim K (2003). P effects on N uptake and remobilization during regrowth of Italian ryegrass (Lolium mutiflorum). Environ Exp Bot 50: 233–242.
Kloepper JW (1994). Plant growth promoting bacteria (other systems). In: Okon J, editor. Azospirillum-Plant Association. Boca Raton, FL, USA: CRC Press, pp. 137–154.
Kloepper JW, Lifshitz K, Schroth MN (1988). Pseudomonas inoculants to benefit plant production. ISI Atlas Sci Anim Plant Sci 1: 60–64.
Kucey RMN (1983). Phosphate-solubilizing bacteria and fungi in various cultivated and virgin Alberta soils. Can J Soil Sci 63: 671–678.
Kundu BS, Gaur AC (1984). Rice response to inoculation with N2fixing and P-solubilizing microorganisms. Plant Soil 79: 227– 2
Lach D, Sharma VK, Vary PS (1990). Isolation and characterization of a unique division mutant of Bacillus megaterium. J Gen Microbiol 3: 545–553.
Lemanceau P (1992). Effets benefiques de rhizobacteries sur les plantes: exemple des Pseudomonas spp. fluorescent. Agronomie 12: 413–437.
Mengel K, Ernest A Harald K, Thomas K (2001). Principles of Plant Nutrition. Dordrecht: Kluwer Academic Publishers.
Mohammed SS, Osman AG, Mohammed AM, Abdalla AS, Sherif AM, Rugheim AME (2012). Effects of organic and microbial fertilization on wheat growth and yield. Int Res J Agric Sci Soil Sci 2: 149–154.
O’Connell PF (1992). Sustainable agriculture a valid alternative. Outlook on Agriculture 21: 5–12.
Orson JA (1996). The sustainability of intensive arable systems; implications for rotational policy. Aspect Appl Biol 47: 11–18.
Rodrigues O, Didonet AD, Gouveia JA, Oares RC (2000). Nitrogen translocation in wheat inoculated with Azosprillium and fertilized with nitrogen. Pesq Agropec Bras, 35: 1473–1481.
Roos W, Luckner M (1984). Relationship between proton extrusion and fluxes of ammonium ions and organic acids in Penicillium cyclopium. J Gen Microbiol 130: 1007–1014.
Saber MSM (1997). Biofertilized farming systems. In: Proceedings of the Training Course on Bio-organic Farming Systems for Sustainable Agriculture (1995, Cairo, Egypt), pp. 66–73.
Saharan BS, Nehra V (2011). Plant growth promoting rhizobacteria: a critical review. Life Scie Med Res 21: 1–30.
Salantur A (2003). Effects of bacteria strains isolated from cereal growing areas of Erzurum and Pasinler valleys on the growth and development of wheat and barley. PhD, Atatürk University, Erzurum, Turkey (in Turkish).
Sayaslan A, Koyuncu M, Yıldırım A, Eserkaya Güleç T, Ateş Sönmezoğlu Ö, Kandemir N (2012). Some quality characteristics of selected durum wheat (Triticum durum) landraces. Turk J Agric For 36: 749–756.
Schilling G, Gransee A, Deubel A, Ležovič G, Ruppel S (1998). Phosphorus availability, root exudates, and microbial activity in the rhizosphere. Zeitschrift für Pflanzenernährung und Bodenkunde 161: 465–478.
Selvakumari G, Basker M, Jayanthi D, Mathan K (2000). Effect of integration of fly ash with fertilizers and organic manures on nutrient availability, yield and nutrient uptake of rice. J Soil Sci Soc 48: 268–278.
Sheng XF, Huang WY (2001). Physiological characteristics of strain NBT of silicate bacterium. Acta Pedol Sinica 38: 569–574.
Singh RR, Prasad K (2011). Effect of bio-fertilizers on growth and productivity wheat (Triticum aestivum). J Farm Sci 1: 1–8.
Smith JH, Allison FE, Soulides DA (1962). Phosphobacteria as a soil inoculant. Tech US Dept Agricult Bul 1: 63–70.
Stevenson FJ (1986). Cycles of Soil: Carbon, Nitrogen, Phosphorus, Sulfur, Micronutrients. New York: John Wiley, p. 231–284.
Suslov TV (1982). Role of root-colonizing bacteria in plant growth. In: Mount MS, Lacy GH, editors. Phytopathogenic Prokaryotes, Vol. London: Academic Press, pp. 187–223.
Şahin F, Çakmakçı R, Kantar F (2004). Sugar beet and barley yields in relation to inoculation with N2-fixing and phosphate solubilizing bacteria. Plant Soil 265: 123–129.
Terman GL, Brown MA (1968). Crop recovery of applied fertilizer nitrogen. Plant Soil 29: 48–65.
Turan M, Gulluce M, Cakmakci R, Oztas T, Sahin F (2010). The effect of PGPR strain on wheat yield and quality parameters. In: Proceedings of 19th World Congress of Soil Science, Soil Solutions for a Changing World, 2010, Brisbane, Australia. Published on DVD, pp. 140–143.
Vazquez P, Holguin G, Puente ME, Lopez-Cortes A, Bashan Y (2000). Phosphate-solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon. Biol Fert Soils 30: 460–468.
Whitelaw MA (2000). Growth promotion of plants inoculated with phosphate solubilizing fungi. Adv Agron 69: 99–151.
Whitelaw MA, Hardenand TA, Bender GL (1997). Plant growth promotion of wheat inoculated with Penicillium radicum sp. nov. Aust J Soil Res 35: 291–300.
Woźniak A (2013). The effect of tillage systems on yield and quality of durum wheat cultivars. Turk J Agric For 37: 133–138.