Zhaoyong SHI, Yinglong CHEN, Xiaogai HOU, Shuangcheng GAO, Fayuan WANG

Arbuscular mycorrhizal fungi associated with tree peony in 3 geographic locations in China

The diversity of arbuscular mycorrhizal fungi (AMF) is of great interest because of their potential function in ecosystems. Tree peony is an important traditional ornamental and medicinal plant with economic significance. We examined the mycorrhizal status of the rhizosphere of 14 common cultivars of tree peony (Paeonia suffruticosa) in 3 different geographic locations in China. Root samples of all cultivars were colonized by AMF. The mean percentage of root length colonization, vesicles, and arbuscules were 39%, 3.6%, and 6.0%, respectively. AMF species richness varied from 5 to 11, and spore density ranged from 20 to 61 per 50 g of rhizospheric soil. The average AMF species diversity (Shannon-Wiener index) was 1.92, ranging from 1.64 to 2.18. A total of 31 AMF species belonging to 3 genera were identified in the rhizospheric soil. Glomus (21) was the dominant genus, followed by Acaulospora (7) and Scutellospora (3). G. aggregatum was the most commonly distributed species, with an occurrence frequency of 71.4 and a relative abundance of 13.6%. This study focused on the comparison of AM fungal diversity associated with tree peony in various original cultivar groups. This knowledge will help in selecting suitable AM inoculums for cultivation in the different original cultivar groups of tree peony.

Anahtar Kelimeler:

Key words: Colonization rate, species diversity, spore density, tree peony

Arbuscular mycorrhizal fungi associated with tree peony in 3 geographic locations in China

Keywords:

Key words: Colonization rate, species diversity, spore density, tree peony,

PDF

___

Aytok Ö, Yılmaz KT, Ortaş İ, Çakan H (2013). Changes in mycorrhizal spore and root colonization of coastal dune vegetation of the Seyhan Delta in the postcultivation phase. Turk J Agric For 37: 52–
Bechem EET, Alexander IJ (2012). Mycorrhiza status of Gnetum spp. in Cameroon: evaluating diversity with a view to ameliorating domestication efforts. Mycorrhiza 22: 99–108.
Biermann B, Linderman RG (1981). Quantifying vesicular– arbuscular mycorrhizae: a proposed method towards standardization. New Phytol 87: 63–67.
Chen DM, Guo N, Guo SX (2010). Effects of arbuscular mycorrhizal fungi on growth and some physiological indices of Paeonia suffruticosa. Acta Bot Boreal-Occident Sin 30: 131–135. (article in Chinese with an abstract in English)
Chen Z, He XL, Guo HJ, Yao XQ, Chen C (2012). Diversity of arbuscular mycorrhizal fungi in the rhizosphere of three host plants in the farming–pastoral zone, north China. Symbiosis 57: 149–160.
Closa I, Goicoechea N (2011). Infectivity of arbuscular mycorrhizal fungi in naturally regenerating, unmanaged and clear-cut beech forests. Pedosphere 21: 65–74.
Dalpé Y (1993). Vesicular-arbuscular mycorrhiza. In: Carter MR, editor. Soil Sampling and Methods of Analysis. Boca Raton, FL, USA: Lewis Publishers, pp. 287–301.
Fan JL, Zhu WX, Kang HB, Ma HL, Tao GJ (2012). Flavonoid constituents and antioxidant capacity in flowers of different Zhongyuan tree peony cultivars. J Funct Foods 4: 147–157.
Gai JP, Christie P, Cai XB, Fan JQ, Zhang JL, Feng G, Li XL (2009). Occurrence and distribution of arbuscular mycorrhizal fungal species in three types of grassland community of the Tibetan Plateau. Ecol Res 24: 1345–1350.
Garmendia I, Mangas VJ (2012). Application of arbuscular mycorrhizal fungi on the production of cut flower roses under commercial-like conditions. Span J Agric Res 10: 166–174.
Guo SX, Liu RJ (2010). Effects of different peony cultivars on community structure of arbuscular mycorrhizal fungi in rhizosphere soil. Chin J Appl Ecol 21: 1993–1997. (article in Chinese with an abstract in English)
Guo SX, Zhang YG, Li M, Liu RJ (2007). AM fungi diversity in the main tree-peony cultivation areas in China. Biodivers Sci 15: 425–431. (article in Chinese with an abstract in English) van der Heijden MGA, Boller T, Wiemken A, Sanders IR (1998a). Different arbuscular mycorrhizal fungi species are potential determinants of plant community structure. Ecology 79: 2082– 20 van der Heijden MGA, Klironomos JN, Ursic M, Moutoglis P, Streitwolf-Engel R, Boller T, Weimken A, Sanders IR (1998b). Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature 396: 69–72.
Hong DY, Pan KY (1999). A revision of the Paeonia suffruticosa complex (Paeoniaceae). Nord J Bot 19: 289–299.
Klironomos JN, McCune J, Hart M, Neville J (2000). The influence of arbuscular mycorrhizae on the relationship between plant diversity and productivity. Ecol Lett 3: 137–141.
Koltai H (2010). Mycorrhiza in floriculture: difficulties and opportunities. Symbiosis 52: 55–63.
Koske RE, Tessier B (1983). A convenient, permanent slide mounting medium. Mycol Soc Am Newsl 34: 59.
Li SL, Zhang YG, Chen DM, Ma J., Guo SX (2009). Effect of arbuscular mycorrhizal fungi on physiology and biochemistry of tree peony under high temperature stress. Chin Agric Sci Bull 2009, 25(7): 154–157. (article in Chinese with an abstract in English)
Lin XG, Feng YZ, Zhang HY, Chen RR, Wang JH, Zhang JB, Chu HY (2012). Long-term balanced fertilization decreases arbuscular mycorrhizal fungal diversity in an arable soil in north China revealed by 454 pyrosequencing. Environ Sci Technol 46: 5764–5771.
Morton JB, Redecker D (2001). Two new families of Glomales, Archaeosporaceae and Paraglomaceae, with two new genera Archaeospora and Paraglomus, based on concordant molecular and morphological characters. Mycologia 93: 181–195.
Ong KH, Chubo JK, King JH, Lee CS, Su DSA, Sipen P (2012). Influence of soil chemical properties on relative abundance of arbuscular mycorrhiza in forested soils in Malaysia. Turk J Agric For 36: 451–458.
Shi ZY, Chen YL, Feng G, Liu RJ, Christie P, Li XL (2006a). Arbuscular mycorrhizal fungi associated with the Meliaceae on Hainan Island, China. Mycorrhiza 16: 81–87.
Shi ZY, Feng G, Christie P, Li XL (2006b). Arbuscular mycorrhizal status of spring ephemerals in the desert ecosystem of Junggar Basin, China. Mycorrhiza 16: 269–275.
Shi ZY, Zhang LY, Li XL, Feng G, Tian CY, Christie P (2007). Diversity of arbuscular mycorrhizal fungi associated with desert ephemerals in plant communities of Junggar Basin, northwest China. Appl Soil Ecol 35: 10–20.
Smith SE, Read DJ (2008). Mycorrhizal Symbiosis. 3rd ed. London: Elsevier Ltd.
Tong RJ, Liu XQ, Geng HR (2010). Effects of arbuscular mycorrhiza fungi on vegetable growth and mineral nutrition of Luoyanqhonq Paeonia suffruticosa seedlings. Guizhou Agric Sci 38(10): 104–106. (article in Chinese with an abstract in English)
Trouvelot A, Kough JL, Gianinazzi-Pearson V (1986). Mesure du taux de mycorhization VA d’un systeme radiculaire. Recherche de methodes d’estimation ayant une signification functionnelle. In: Gianinazzi-Pearson V, Gianinazzi S, editors. Physiological and Genetic Aspects of Mycorrhizae. Paris: INRA Press, pp. 217–221.
Tüfenki Ş, Demir S, Şensoy S, Ünsal H, Demirer E, Erdinç Ç, Biçer Ş, Ekincialp A (2012). The effects of arbuscular mycorrhizal fungi on the seedling growth of four hybrid cucumber (Cucumis sativus L.) cultivars. Turk J Agric For 36: 317–327.
Wang LS, Hashimoto F, Shiraishi A, Aoki N, Li JJ, Sakata Y (2004). Chemical taxonomy of the Xibei tree peony from China by floral pigmentation. J Plant Res 117: 47–55.
Yamato M, Yagame T, Yoshimura Y, Iwase K (2012). Effect of environmental gradient in coastal vegetation on communities of arbuscular mycorrhizal fungi associated with Ixeris repens (Asteraceae). Mycorrhiza 22: 623–636.