MEHMET ZAHİT YEKEN, GÖKSEL ÖZER, ALİ ÇELİK, VAHDETTİN ÇİFTÇİ

Tescilli Fasulye Çeşitlerinin Pas (Uromyces appendiculatus) Etmenine Karşı Dayanıklılık Durumlarının SCAR Markörleri ile Belirlenmesi

Fasulye pas hastalığı tüm dünyada fasulye üretiminde sorun oluşturan önemli fungal hastalıklardan birisidir. Fasulye pası ile en etkili ve çevre açısından en zararsız mücadele şekli dayanıklı çeşit ıslahı ve kullanımıdır. Son yıllarda geliştirilen moleküler markörler sayesinde hastalıklara karşı dayanıklılık önceden belirlenebilmekte ve ıslah programları bu veriler ışığında planlanabilmektedir. Bu çalışmada, Türkiye’de yaygın olarak yetiştiriciliği yapılan 43 tescilli fasulye çeşidinin pas hastalığına dayanıklılık durumları SCAR markörleri ile incelenmiştir. Dayanıklılıkla ilgili 4 farklı markör (SA14, SI19, SBC6, AE19) yardımıyla yapılan PCR çalışmaları bazı çeşitlerin pas hastalığına karşı dayanıklılık sağlayan genlere sahip olduklarını ortaya çıkarmıştır. Elde edilen tarama sonuçlarının mevcut çeşitlerin dayanıklılık durumlarını ortaya koymasının yanı sıra, pas ile ilgili yapılacak moleküler destekli dayanıklılık ıslahı çalışmalarının temelini oluşturacağı ön görülmektedir.

Anahtar Kelimeler:

Pas, Uromyces appendiculatus

Determination of the Resistance to the Bean Rust (Uromyces appendiculatus) in Registered Bean Varieties by Using SCAR Markers

Bean rust disease is one of the most important fungal diseases of limiting the yields of common bean all over the world. The most effective and the most harmless methods against the bean rust pathogen are breeding and use of the resistant varieties. Resistance to diseases can be previously determined in breeding programs with the critical data obtained from molecular markers developed in recent years. In this study, resistance against bean rust was investigated by SCAR markers in 43 registered bean varieties, which is widely cultivated in Turkey. As a result, PCR studies performed with 4 different markers (SA14, SI19, SBC6, AE19) which characterize the genes related to resistance revealed that some varieties have the genes linked to the resistance. In addition to revealing the current status of the registered varieties, the results of the study are expected to support new breeding programs.

Keywords:

Bean rust, Uromyces appendiculatus,

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