Doğada ve Kültür Ortamındaki Çoruh alabalığı (Salmo coruhensis) Spermatozoasının Oksidatif Stres ve Antioksidan Biyobelirteçlerinin Belirlenmesi
Balıklarda davranış ve fizyoloji çevresel faktörlerden etkilenmektedir. Özellikle, kültür ortamı ve habitat sucul canlıların yaşam safhaları için önemlidir. Bu çalışmada, doğadaki ve çiftlik ortamındaki balıkların spermatozoasındaki oksidatif stres ve antioksidan biyomarkırları karşılaştırılmıştır. Çoruh alabalığının (Salmo coruhensis) spermatozasında enzimatik antioksidan aktiviteleri (süperoksit dismutaz, katalaz, glutatyon peroksidaz), glutatyon ve lipid peroksidasyonu (malondialdehit) belirlenmiştir. Sonuçlar süperoksit dismutaz (0.44±1.25 Umg-1 protein), katalaz (15.21±4.69 k.g-1 protein), glutatyon peroksidaz 42.89±0.61 Ug-1 protein), glutatyon (0.18±4.01 μmolg-1 hücre) ve malondialdehit (2.49±0.12 nmolg-1 hücre) seviyelerinin kültür ortamındakilerle karşılaştırıldığında doğadaki balık spermatozoasında düşük olduğunu göstermiştir. Sonuç olarak, habitattaki farklılıklar antioksidan durumu ve malondialdehit seviyelerinde değişikliklere neden olabilir.
Assessment of Biomarkers of Oxidative Stress and Antioxidant in Wild and Farmed Çoruh trout (Salmo coruhensis) Spermatozoa
Behaviour and physiology in fish are affected from environmental factors. Particularly, habitat and culture environment in aquatic animals are essential for life stages. In this study, biomarkers of oxidative stress and antioxidant were compared in wild and farmed Çoruh trout (Salmo coruhensis) spermatozoa. In spermatozoa of Çoruh trout, lipid peroxidation (malondialdehyde), glutathione and enzymatic antioxidant activities (catalase, superoxide dismutase, glutathione peroxidase) were assessed. Our data demonstrated that superoxide dismutase (0.44±1.25 Umg-1 protein), catalase (15.21±4.69 k.g-1 protein), glutathione peroxidase (42.89±0.61 Ug-1 protein), glutathione (0.18±4.01 μmolg-1 cell) and malondialdehyde (2.49±0.12 nmol.g-1 cell) levels in wild fish were low compared to spermatozoa of farmed fish. Overall, alterations in malondialdehyde levels and the antioxidant status could be differences in habitat.
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