Bazı İlaç ve Metallerin Keçi (Capra aegagrus hircus) Böbreğinde Aldoz Redüktaz ve Sorbitol Dehidrogenaz Enzimleri Üzerine Etkilerinin Araştırılması

Bazı antibiyotik ve metallerin keçi (Capra aegagrus hircus) böbrek aldoz redüktaz (AR) ve sorbitol dehidrogenaz (SDH) aktiviteleri üzerine etkileri incelenmiştir. İnhibitör etki gösteren ilaç ve metaller için Ki sabitleri hesaplanmış ve Lineweaver-Burk eğrileri kullanılarak inhibisyon tipleri belirlenmiştir. Çalışmamızda AR enziminde en yüksek inhibisyon, 0.0274 mM IC50 değeri ile seftriakson antibiyotiği gösterdi. Seftriakson bileşiğinden daha güçlü AR inhibitörleri sentezlenebilir. Bu bileşiği IC50 değerleri sırasıyla 0.061 ve 0.25 mM olan amikasin sülfat ve siproflaksasin bileşikleri izlemektedir. Metallerin en yüksek inhibisyonu, 0.000445 mM IC50 değeri ile Co+2 iyonu göstermiştir. Bu metali sırasıyla 0.009 ve 1.43 mM IC50 değerleri ile FeSO4 ve MgCl2 takip etmektedir. SDH enziminde en yüksek inhibisyon, 0.016 mM IC50 değeri ile rifamisin sodyum antibiyotik gösterdi. Rifamisin sodyum bileşiğinden daha güçlü SDH inhibitörleri sentezlenebilir. Bu bileşiği sırasıyla 0.025 ve 0.16 mM IC50 değerlerine sahip seftriakson ve sefuroksim bileşikleri takip etmektedir. Metallerin en yüksek inhibisyonu 0.00044 mM IC50 değeri ile Co+2 iyonunu göstermiştir. Bu metali sırasıyla 0.009 ve 0.16 mM IC50 değerleri ile Fe+2 ve Zn+2 takip etmektedir.

Anahtar Kelimeler:

Aldoz redüktaz, sorbitol dehidrogenaz, keçi böbreği, ilaçlar, enzim inhibisyonu

Investigation of Some Drugs and Metals Effects on Aldose Reductase and Sorbitol Dehydrogenase Enzymes from Goat (Capra aegagrus hircus) Kidney

The effects of some antibiotics and metals on goat (Capra aegagrus hircus) kidney aldose reductase (AR) and sorbitol dehydrogenase (SDH) activities were examined. For drugs and metals that exhibit inhibitory effect, Ki constants were calculated and inhibition types were determined by using Lineweaver-Burk curves. In our study, the highest inhibition showed ceftriaxone antibiotic in AR enzyme with an IC50 value of 0.0274 mM. More potent AR inhibitors can be synthesized from the ceftriaxone compound. This compound is followed by amikacin sulfate and ciproflaksasin compounds with IC50 values of 0.061 and 0.25 mM, respectively. The highest inhibition of metals showed Co+2 ion with IC50 value 0.000445 mM. This metal is followed by Fe+2 and Zn+2 with IC50 values of 0.0286 and 0.084 mM, respectively. In SDH enzyme, the highest inhibition showed rifamycin sodium antibiotic with an IC50 value of 0.016 mM. More potent SDH inhibitors can be synthesized from the rifamycin sodium compound. This compound is followed by ceftriaxone and cefuroxime compounds with IC50 values of 0.025 and 0.16 mM, respectively. The highest inhibition of metals showed Co+2 ion with IC50 value 0.00044 mM. This metal is followed by Fe+2 and Zn+2 with IC50 values of 0.009 and 0.16 mM, respectively.

Keywords:

Aldose reductase, sorbitol dehydrogenase, goat kidney, drugs, enzymes inhibition,

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