Asphodelus ramosus, Allium cepa L. ve Allium porrum L. Bitkilerinin Antioksidan Kapasitesi ve Fenolik Bileşenlerin HPLC ile Ayrımı

Sarızambak olarak bilinen ve latince adı Asphodelus Ramosus olan, çiriş bitkisinin antioksidan özellikleri incelenmiştir. Bu amaçla metanol, % 50 metanol, etil asetat ve su ekstraktları ile çalışılmıştır. Bitki iki farklı sıcaklıkta kurutulup (35 ºC ve 65 ºC ), böylece sıcaklık farklılığının bir parametre oluşturması sağlanmıştır. Ekstraksiyon işleminden sonra tüm ekstraktların toplam fenolik bileşenlerine Folin Ciocalteu yöntemi ve toplam flavonoid bileşenlerine aluminum nitrat metodu uygulanmıştır. Buna ilaveten DPPH serbest radikal giderici etkisi, H2O2 süpürücü etkisi, bakır (II) iyonu indirgeme gücü (CUPRAC), süperoksit anyon radikali süpürme kapasitesi analiz edilmiş ve tüm bunlar HPLC (Yüksek Performanslı Sıvı Kromatografi) ile desteklenmiştir.

Anahtar Kelimeler:

Asphodelus ramosus, ekstraksiyon, flavonoid, DPPH, H2O2, CUPRAC, HPLC

Antioxidant Capacity and Phenolic Compounds with HPLC of Asphodelus ramosus and Comparison of the Results with Allium cepa L. and Allium porrum L. Extracts

Asphodelus ramosus known as a yellow lily which has been a plant of onion for this reason, this plant was studied with about antioxidant activity of methanol, 50 % methanol, ethyl acetate and water extracts. The plant was dried at two different temperatures (35 ºC and 65 ºC) so as to understand how to work the difference in temperature effects. After extraction, all solvent extracts were studied for their total phenolic contents using Folin Cioceltau reagent, total flavonoid content with aluminum nitrate method. In addition, DPPH free redical scavenging through the determination, the determination of the activity of removal of H2O2, copper (II) ion reducing antioxidant capacity determination (CUPRAC), removal activity of super oxide anion were studied and the results were supported with HPLC (High Performance Liquid Chromatography).

Keywords:

Asphodelus ramosus, extraction, flavonoid, DPPH, H2O2, CUPRAC, HPLC,

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