The Effects of Age and Number of Grafted Larvae on Some Physical Characteristics of Queen Bees and Acceptance Rate of Queen Bee Cell

This study was carried out to determine the effects of age and number of grafted larvae on acceptance rates, body weight, body length, head width and length, thorax width and length, and wing width and length of queen bees. One breeding and eleven starter hives were used for rearing queen bees. Totally, 495 one, two, and three-day-old larvae were grafted into starter hives comprising 30, 45, and 60 and 414 larvae were accepted; thus, generally, the acceptance rate was calculated as 83%. The best acceptance rate was calculated in three-day-old larvae group as 85.15%. There were found a statistically significant effect of the number of grafted larvae on body length and head width of queen. However, there were any effects on weight, head length, thorax width, thorax length, wing width, wing length, and acceptance rate of larvae. Age of grafted larvae did not have a statistically significant effect on head width, head length, wing width and acceptance rate of larvae. On the other hand, age of grafted larvae had a statistically significant effect on queen weight, body length, thorax width and length, and wing length of queen. If bee breeders wish to improve their stock, they should graft one-day-old larvae for rearing better queen bees.

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Abrol DP, Bhagat R, Sharma D. 2005. Mass Rearing of Apis cerana F. Queen. Journal of Asia-Pacific Entomology, 8(3): 309-317. doi:10.1016/s1226-8615(08)60251-4

Adam B. 1983. In Search of the Best Strains. Hebden Bride, UK: Northern Bee Books.

Akyol E, Yeninar H, Korkmaz A, Çakmak I. 2008. An observation study on the effects of queen age on some characteristics of honey bee colonies. Italian Journal of Animal Science, 7(1): 19-25. doi:10.4081/ijas.2008.19

Akyol E, Yeninar H, Kaftanoglu O. 2008. Live Weight of Queen Honey Bees (Apis Mellifera L.) Predicts Reproductive Characteristics. Journal of the Kansas Entomological Society, 81(2): 92-100. doi:10.2317/jkes- 705.13.1

Ali MA. 2012. Studies on bee venom and its medical uses. International Journal of Advancements in Research & Technology, 69-83.

Avetisyan GA. 1961. The relation between interior and exterior characteristics of the queen and fertility and productivity of the bee colony. In: XVIII International Beekeeping Congress, pp. 44-53.

Chang CC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis.

Dedej S, Hartfelder K, Aumeier P, Rosenkranz P, Engels W. 1998. Caste determination is a sequential process: Effect of larval age at grafting on ovariole number, hind leg size and cephalic volatiles in the honey bee (Apis mellifera carnica). Journal of Apicultural Research, 37(3): 183-190. doi:10.1080/00218839.1998.11100970

Delaney DA, Keller JJ, Caren JR, Tarpy DR. 2011. The physical, insemination, and reproductive quality of honey bee queens (Apis mellifera L.). Apidologie, 42(1): 1-13. doi:10.1051/apido/2010027

Dodologlu A, Emsen B, Gene F. 2004. Comparison of Some Characteristics of Queen Honey Bees (Apis melliferaL.) Reared by Using Doolittle Method and Natural Queen Cells. Journal of Applied Animal Research, 26(2): 113-115. doi:10.1080/09712119.2004.9706518

Eckert JE. 1934. Studies in the Number of Ovarioles in Queen Honeybees in Relation to Body Size. Journal of Economic Entomology, 27(3): 629-635. doi:10.1093/jee/27.3.629

Eckert JE. 1937. Relation of Size to Fecundity in Queen Honeybees. Journal of Economic Entomology, 30(4): 646- 648.

Emsen B. 2004. Relationship between larval age and characteristics of queen honey bees (Apis mellifera L.) after single and double grafting. In Proceeding of the 1st European Conference of Apidologie, 66.

FAO. 2010. FAO Statistics Division Food Balance Sheets. Food and Agriculture Organization of the United Nations.

Fischer F, Maul V. 1991. Untersuchungen zu aufzuchtbedingten königinnenmerkmalen. Aninquiry into the characteristics of queens depending on queen rearing. Apidologie, 22: 444- 446.

Fukuda H, Ohtani T. 1977. Survival and life span of drone honeybees. Researches onPopulation Ecology, 19(1), 51-68. doi:10.1007/bf02510939

Fukuda H, Sekıguchı K. 1966. Seasonal change of the honeybee worker longevity in Sapporo, North Japan, with notes on some factors affecting the life-span. Japanese Journal of Ecology, 16(5): 206-212.

Genc F. 1992. A study on determination of the effects of using different ages queens on colony performance. Proc. Ist Beekeeping Seminar East Anatolia, Erzurum, Turkey, 77- 95.

Vasfi Gençer H, Qasim Shah S, Firatli Ç. 2000. Effects of Supplemental Feeding of Queen Rearing Colonies and Larval Age on the Acceptance of Grafted Larvae and Queen Traits. Pakistan Journal of Biological Sciences, 3(8): 1319- 1322. doi:10.3923/pjbs.2000.1319.1322

Gilley DC, Tarpy DR, Land BB. 2003. Effect of queen quality on interactions between workers and dueling queens in honeybee (Apis mellifera L.) colonies. Behavioral Ecology and Sociobiology, 55(2): 190-196.

Güler A. 2006. Bal Arisi. Samsun: OMU Ziraat Fakultesi Ders Kitabi (55): 9-11.

Güler A, Kaftanoğlu O. 1999. Determination of Performances Some Important Races and Ecotypes of Turkish Honeybees (Apis mellifera L.) Under Migratory Beekeeping Conditions. Turkish Journal of Veterinary and Animal Sciences, 23(EK3): 577-582.

Harry H. Laidlaw J. 1978. Contemporary queen rearing. California, Davis: Royal Printing.

Hatch S, Tarpy DR, Fletcher D . 1999. Worker regulation of emergency queen rearing in honey bee colonies and the resultant variation in queen quality. Insectes Sociaux, 46(4): 372-377. doi:10.1007/s000400050159

Eissa IS, Hussain AE, Shehata IAA, Helaly KIM. 2007. Study of certain factors affecting queen rearing in honeybee colonies on the acceptance rate of grafted queen cells. Egypt: El-Azhar, Univ.

Kaftanoğlu O, Düzenli A, Kumova U. 1988. A study on determination of the effects of queen rearing season on queen quality under Çukurova Region conditions. Turkish Journal of Veterinary and Animal Sciences, 16(3): 567-577.

Kaftanoğlu OK. 1992. Ana arı yetiştiriciliğinin önemi ve ana arının kalitesini etkileyen faktörler. Arıcılık Semineri.

Kahya Y, Gençer HV, Woyke J. 2008. Weight at emergence of honey bee (Apis mellifera caucasica) queens and its effect on live weights at the pre and post matingperiods. Journal of Apicultural Research, 47(2): 118-125.

Koç AU, Karacaoglu M. 2004. Effects of Rearing Season on The Quality of Queen Honeybees (Apis Mellifera L.) Raised Under The Conditions of Aegean Region. Mellifera, p34-37.

Korkmaz A. 2005. Ana Ari Yetiştiriciliği. Samsun: Turkish Ministry of Agriculture.

Krell R. 1996. Value-added products from beekeeping. Food & Agriculture Org.

Laidlaw HHPR. 1997. Queen Rearing and Bee Breeding. Cheshire: Wicwas Press.

Laidlaw HH. 1978. Contemporary Queen Rearing. California.

Laidlaw HH. 1992. Production of queens and package bees. The hive and the honey bee, 989-1042.

Levin MD. 1983. Value of bee pollination to US agriculture. American Entomologist, 29(4), 50-51.

Mahbobi A, Farshineh-Adl M, Woyke J, Abbasi S. 2012.

Effects of the Age of Grafted Larvae and the Effects of Supplemental Feeding on Some Morphological Characteristics of Iranian Queen Honey Bees (Apis mellifera meda Skorikov, 1929). Journal of Apicultural Science, 56(1). doi:10.2478/v10289-012-0010-1

Mark W. 1987. The Biology of the Honey Bee. Cambridge: Harvard University Press.

Metorima F N, Costa-Maia F M, Halak A L, Parpinelli R S, Vagner De Alencar Arnaut De Toledo. 2015. Morphometric measurements of Africanized honeybee queens kept in an incubator or in queen banking. Acta Scientiarum. Animal Sciences, 37(1): 91. doi:10.4025/actascianimsci. v37i1. 24006

Mirza E, Dragan M, Sherbanescu S. 1967. Seasonal variability in the weight of emerging queens. In The 21st International Apiculture Congress of Apimondia (pp. 269-273).

Masry SHD, El-Wahab TA, Hassona NM. 2015. Origin, Weight at Emergence of Virgin Honey Bee Queens and its Effect on Acceptance During Introduction. Academic Journal of Entomology, 8: 174-182.

Nelson DL, Gary NE. 1983. Honey Productivity of Honey Bee Apis-Mellifera Colonies in Relation to Body Weight Attractiveness and Fecundity of the Queen. Journal of Apicultrual Research, 22: 209-213.

Özturk AI. 2014. Quality concept in honey bee queen and factors affecting of the its quality. Journal of Aegean Agricultural Research Institute, 59-65

Page RE, Peng CY. 2001. Aging and development in social insects with emphasis on the honey bee, Apis mellifera L. Experimental Gerontology, 36(4-6): 695-711. doi:10.1016/s0531-5565(00)00236-9

Ribbands CR. 1953. The behaviour and social life of honeybees. Bee Research Association Limited.; London.

Richard F, Tarpy DR, Grozinger CM. 2007. Effects of Insemination Quantity on Honey Bee Queen Physiology. PLoS ONE, 2(10). doi:10.1371/journal.pone.0000980

Rothenbuhler WC. 1958. Genetics and breeding of the honey bee. Annual Review of Entomology, 3(1): 161-180.

Smith KM, Loh EH, Rostal MK, Zambrana-Torrelio CM., Mendiola L, Daszak P. 2013.

Pathogens, pests, and economics: drivers of honey bee colony decline and losses. EcoHealth, 10(4): 434-445.

Tarpy D, Page R. 2000. No Behavioral Control over Mating Frequency in Queen Honey Bees (Apis mellifera L.): Implications for the Evolution of Extreme Polyandry. The American Naturalist, 155(6), 820. doi:10.2307/3079103

Tarpy DR, Keller JJ, Caren JR, Delaney DA. 2011. Experimentally induced variation in the physical reproductive potential and mating success in honey bee queens. Insectes Sociaux, 58(4): 569-574. doi:10.1007/s00040-011-0180-z

Thorp RW. 2000. The collection of pollen by bees. Pollen and Pollination, 211-223. doi:10.1007/978-3-7091-6306-1_11

Weaver N. 1957. Effects of Larval Age on Dimorphic Differentiation of the Female Honey Bee1.Annals of the Entomological Society of America, 50(3): 283-294. doi:10.1093/aesa/50.3.283

Wen-Cheng H, Chong-Yuan Z. 1985. The relationship between the weight of the queen honey bee at various stages and the number of ovarioles, eggs laid and sealed brood produced. Honey Bee Science, 6(3): 113-116.

Wilson EO. 1971. The insect societies. University Press, Cambridge.

Winston M L, Dropkin J A, Taylor O R. 1981. Demography and life history characteristics of two honey bee races (Apis mellifera). Oecologia, 48(3): 407-413. doi:10.1007/ bf00346502

Winston ML, Taylor OR, Otis GW. 1983. Some Differences between Temperate European and Tropical African and South American Honeybees. Bee World, 64(1): 12-21. doi:10.1080/0005772x.1983.11097902

Woodward DR. 2007. Queen Bee: Biology, rearing and breeding. Telford Rural Polytechnic.

Woyke J. 1967. Rearing conditions and number of sperms reaching the queens spermatheca. In Proceedings of the XXI (s. 84). International Congress Apimondia.

Woyke J. 1971. Correlations Between the Age at Which Honeybee Brood was Grafted, Characteristics of the Resultant Queens, and Results of Insemination. Journal of Apicultural Research, 10(1): 45-55. doi:10.1080/ 00218839.1971.11099669