HATIRA TAŞKIN, TÜLİN EKER, FUAT BOZOK, HASAN HÜSEYİN DOĞAN, Saadet BÜYÜKALACA

Determination of Multiple Antioxidant Activities of Endemic Tricholoma anatolicum H.H Doğan & Intini Collected from Turkey

Tricholoma anatolicum H.H. Doğan & Intini is known as the most prized mushroom species in Feke region of Turkey. This mushroom species is collected from Cedar (Cedrus libani) forests and therefore is named as Cedar mushroom in this region. It is collected and consumed by local collectors and also exported to Far East countries such as Japan by exporting companies. Therefore, it is important to investigate the nutritional and medical importance of this species. The aim of this study is to reveal the antioxidant activity of methanol extract in different concentrations (1, 2 and 4 mg/mL) of T. anatolicum H.H. Doğan & Intini collected from Feke district of Adana province of Turkey in 2015. In this study, it was determined that total phenolic content of this mushroom was 56 mg/kg. 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and reducing power (RP) activities given in trolox (μM) and ferric-reducing antioxidant power (FRAP) given in FeSO4 (μM) were found as 449, 180, 337 and 2 at the highest concentration (4 mg/mL), respectively. As a result, it could be suggested that methanol extract of T. anatolicum H.H. Doğan & Intini has significantly antioxidant activity.

Türkiye’den Toplanan Endemik Tricholoma anatolicum’un Çoklu Antioksidant Aktivitesinin Belirlenmesi

Tricholoma anatolicum H.H. Doğan & Intini Adana’nın Feke ilçesinde en değerli mantar türü olarak bilinmektedir. Bu mantar türü Sedir (Cedrus libani) ormanlarından toplanmaktadır ve bu nedenle bu bölgede sedir mantarı olarak adlandırılmaktadır. Bölgede lokal halk tarafından toplanarak tüketilmekte ve aynı zamanda Japonya gibi Uzak Doğu ülkelerine ihracat firmaları tarafından ihraç edilmektedir. Bu nedenle, bu türün besleyici ve tıbbi özelliklerini araştırmak önemlidir. Bu çalışmanın amacı, 2015 yılında Adana ilinin Feke ilçesinden toplanan T. anatolicum türünün farklı konsantrasyonlarda (1, 2 ve 4 mg/mL) metanol ekstraktının antioksidan aktivitesini ortaya çıkarmaktır. Çalışma sonunda, bu mantarın toplam fenolik içeriği 56 mg/kg olarak tespit edilmiştir. Trolox’da verilen (μM) 2,2-difenil-1-pikrilhidrazil (DPPH), nitric oxide (NO) ve indirgeme gücü (RP) aktiviteleri ve FeSO4 (μM)’de verilen ferrik indirgeyici antioksidan (FRAP); en yüksek konsantrasyonda (4 mg/mL) sırasıyla 449, 180, 337 ve 2 olarak bulunmuştur. Sonuç olarak, T. anatolicum'un metanol ekstraktının önemli ölçüde antioksidan aktiviteye sahip olduğu söylenebilir.

PDF

___

Akgül H, Nur AD, Sevindik M, Doğan M. 2016. Tricholoma terreum ve Coprinus micaceus’un bazı biyolojik aktivitelerinin belirlenmesi, Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 17(2): 158-162.

Barros L, Baptista P, Ferreira ICFR. 2007. Effect of Lactarius piperatus fruiting body maturity stage on antioxidant activity measured by several biochemical assays, Food Chem. Toxicol., 45: 1731-1737.

Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay, Anal. Biochem., 239: 70-76.

Benzie IFF, Szeto YT. 1999. Total antioxidant capacity of teas by the ferric reducing / antioxidant power assay, J. Agric. Food Chem., 47: 633-636.

Bozdoğan A, Eker T, Bozok F, Ulukanlı Z, Doğan HH, Büyükalaca S. 2016. Multiple antioxidant and bioherbicidal assays of the edible mushroom species “Ramaria flava” in the Amanos mountains, Biointerface Res. Appl. Chem., 6(6): 1681-1685.

Bozok F, Eker T, Sezer G, Bozdoğan A, Doğan HH, Büyüklaca S. 2016. Investigation of antioxidant potential and phytotoxic effects of Ganoderma lucidum methanol extract, Turjaf, 4(3): 163-170.

Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity, Food Sci. Technol. Int., 28(1): 25-30.

Chan MMY, Fong D, Ho CT, Huang HI. 1997. Inhibition of inducible nitric oxide synthase gene expression and enzyme activity by epigallocatechin gallate, a natural product from green tea, Biochem. Pharmacol, 54: 1281-1286.

Doğan HH, Akata I. 2011. Ecological features of Tricholoma anatolicum in Turkey, Afr. J. Biotechnol, 10(59): 12626-12668.

Doğan HH, Şanda MA, Akata I. 2012. Mn, Fe, K, Na, and P contents in some Tricholoma (Fr.) Staude (Tricholomataceae) taxa from central Anatolia, Turkey, Fresen Environ Bull, 21: 3389-3393.

Elmastas M, Işıldak O, Türkekul I, Temur N. 2007. Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms, J. Food Compos. Anal, 20: 337-345.

Garratt DC. 1964. The quantitative analysis of drugs (Vol. 3), Chapman and Hall Ltd, Japan.

Gürsoy N, Sarıkürkçü C, Cengiz M, Solak MH. 2009. Antioxidant activities, metal contents, total phenolics and flavonoids of seven Morchella species. Food Chem. Toxicol, 47: 2381-2388.

Halliwell B, Gutteridge JHMC. 1984. Oxygen toxicity, oxygen radicals, transition metals and disease, J. Biochem, 219: 1- 14. Halliwell B. 1996. Antioxidants in human health and disease, Annu. Rev. Nutr, 16: 33-50.

Kalmış E, Yıldız H, Ergönül B, Kalyoncu F, Solak MH. 2011. Chemical composition and nutritional value of a wild edible ectomycorrhizal mushroom, Tricholoma anatolicum. Turk J Biol, 35: 627-633.

Moyer RA, Hummer KE, Finn CE, Frei B, Wrolstad RE. 2002. Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: Vaccinium, Rubus, and Ribes, J. Agric. Food Chem, 50: 519-525.

Nakayama T, Yamada M, Osawa T, Kawakiskhi S. 1993. Suppression of active oxygen-induced cytotoricity by flavonoids, Biochem. Pharmacol, 45: 265-267.

Oyaizu M. 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine, Japanese Journal of Nutrition, 44: 307-315.

Özdemir Ö. 2014. Determination of anticancer and modulation effects of Terfezia boudieri Chatin, Fomes fomentarius (L.) FR., Phellinus igniarius (L.) QUÉL. and Tricholoma anatolicum H.H. Doğan & Intini on breast cancer cells, MSc Thesis, Konya, Turkey.

Özkaya D. 2015. Hepatoprotective effects against H2O2-induced damage in HEPG2 cells of Tricholoma anatolicum H.H. Doğan & Intini, MSc Thesis, Konya, Turkey.

Rice-Evans CC, Miller NJ, Paganpa G. 1997. Antioxidant properties of phenolic compounds, Trends Plant Sci, 2(4): 152-159.

Smolskaitė L, Venskutonis PR, Talou T. 2015. Comprehensive evaluation of antioxidant and antimicrobial properties of different mushroom species. LWT-Food Sci. Techn, 60(1): 462-471.

Stacewicz-Sapuntzakis M, Bowen PE, Hussain EA, Damayanti- Wood BI, Farnsworth NR. 2001. Chemical composition and potential health effects of prunes: a functional food?, Crit. Rev. Food Sci, 41(4): 251-286.

Taşkın H, Büyükalaca S. 2016. Minimally processed mushrooms. Food engineering series book series (FSES), Minimally Processed Refrigerated Fruits and Vegetables pp 433-468.

Visioli F, Borsani L, Galli C. 2000. Diet and prevention of coronary heart disease: The potential role of phytochemicals, Cardiovasc. Res, 47: 419-425.

Vriesman MF, Paquay JBG, Voss H. 1997. A method for measuring nitric oxide radical scavenging activity. Scavenging properties of sulfur-containing compounds, Pharm. World Sci, 19(6): 283-286.

Wettasinghe M, Shahidi F. 1999. Antioxidant and free radicalscavenging properties of ethanolic extracts of defatted borage (Borago officinalis L.) seeds. Food Chem, 67: 399- 414.

Wong CC, Li HB, Cheng KW, Chen F. 2006. A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay, Food Chem, 97: 705-711.

Yang JH, Lin HC, Mau JL. 2002. Antioxidant properties of several commercial mushrooms, Food Chem, 77(2): 229- 235.