MUHAMMET ENİS YONAR, NAİM SAĞLAM, SEVAL YILMAZ

Propolis Enjekte Edilen Gökkuşağı Alabalığı (Oncorhynchus mykiss)’nda Bazı İmmünolojik Parametrelerin İncelenmesi

Bu çalışmada; gökkuşağı alabalığında bazı immunolojik parametreler üzerine propolisin etkisi araştırıldı. Bu amaçla, propolis 2,5; 5 ve 10 mg/kg balık ağırlığı dozunda 4 defa balıklara intraperitoneal olarak enjekte edildi. Deneme ve kontrol grubundaki balıklardan 3., 9., 15., ve 21. günlerde kan örnekleri alındı oksidatif radikal üretimi [nitrobluetetrazolium (NBT) aktivitesi], total protein ve total immunoglobulin düzeyleri analiz edildi. Deneme sonunda, kontrol grubuna göre propolis uygulanan grupların oksidatif radikal üretimi, total protein ve immunoglobulin düzeylerinde istatistiksel olarak önemli bir artış tespit edildi.

Investigation of Some Immunological Parameters in Rainbow Trout (Oncorhynchus mykiss) Injected Propolis

In this study, effects of propolis on immunological parameters of rainbow trout were investigated. For this purpose, propolis was intraperitoneally injected to fish 4 times at doses of 2.5, 5 and 10 mg / kg fish weight. Blood samples on 3, 9, 15 and 21 days were collected from fish of the control and experimental groups and analysed to determine oxidative radical production [nitroblue tetrazolium (NBT) activity], total plasma protein and total immunoglobulin levels. At the end of the experiment, there was a statistically significant increase in oxidative radical production, total protein and immunoglobulin levels of propolis treated groups when compared to the control group.

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Abd-El-Rhman AMM. 2009. Antagonism of Aeromonas hydrophila by propolis and its effect on the performance of Nile tilapia, Oreochromis niloticus. Fish Shellfish Immunol., 27: 454-459. DOI: 10.1016/j.fsi.2009.06.015.
Arda M, Minbay A, Aydın N, Akay Ö, İzgür M, Diker KS. 1994. İmmunoloji. Ankara: Medisan Yayınevi.
Cuesta A, Rodriguez A, Esteban MA, Meseguer J. 2005. In vıvo effects of propolis, a honeybee product, on gilthead seabream innate immun responses. Fish Shellfish Immunol., 18: 71-80. DOI: 10.1016/j.fsi.2004.06.002
Dalmo RA, Ingebrigtsen K, Bogwald J. 1997. Non-specific defence mechanisms in fish, with particular reference to the reticuloendothelial system (RES). J Fish Dis., 20: 241- 273. DOI: 10.1046/j.1365-2761.1997.00302.x
Diker S. 1998. İmmunoloji. Ankara: Medisan Yayınevi.
Dimov V, Ivanovska N, Manolova N, Bankova V, Nikolov N, Popov S. 1991. Immunomodulatory action of propolis. Influence on anti-infectious protection and macrophage function. Apidologie, 22: 155-162.
Ellis AE. 1988. Vaccination against enteric redmouth (ERM). In: Ellis AE. (ed). Fish vaccination. London: Academic Press. pp 85-92.
Eraslan G, Kanbur M, Silici S. 2007. Evaluation of propolis effects on some biochemical parameters in rats treated with sodium fluoride. Pestic Biochem Phys., 88: 273-283. DOI: 10.1016/j.pestbp.2007.01.002
Fuliang HU, Hepburn HR, Hongzhuan X, Minli C, Daya S, Radloff S. 2005. Effect of propolis on blood glucose, blood lipid and free radicals in rats with diabet mellitus. Pharmacol. Res., 51: 147-152. DOI: 10.1016/j.phrs. 2004.06.011
Ivanovska N, Dimov VB, Pavlova S, Bankova VS, Popov S. 1995. Immunomodulatory action of propolis. V. Anticomplemantary activity of a water-soluble derivative. J Ethnopharmacol., 47: 135-143. DOI: 10.1016/0378- 8741(95)01273-G
Jeney G, Galeotti M, Volpatti D, Jeney Z, Anderson D.P. 1997. Prevention of stress in rainbow trout ( Oncorhynchus mykiss) fed diets containing different doses of glucan. Aquaculture, 154: 1-15. DOI: 10.1016/S0044-8486(97) 00042-2.
Katırcıoğlu H, Mercan N. 2006. Antimicrobial activity and chemical compositions of Turkish propolis from different region. Afr. J. Biotechnol., 5: 1151-1153.
Mişe Yonar S, Ural MŞ, Silici S, Yonar ME. 2014. Malathioninduced changes in the haematological profile, the immune response, and the oxidative/antioxidant status of Cyprinus carpio carpio: Protective role of propolis. Ecotox Environ Safe., 102: 202-209. DOI: 10.1016/j.ecoenv.2014.01.007.
Özkul Y, Silici S, Eroğlu E. 2005. The anticarcinogenic effect of propolis in human lymphocytes culture. Phytomedicine, 12: 742-747. DOI: 10.1016/j.phymed.2004.06.015.
Sağlam N, Yonar ME. 2009. Effects of sulfamerazine on selected haematological and immunological parameters in rainbow trout (Onchorhynchus mykiss, Walbaum, 1792). Aquac Res., 40: 395-404. DOI: 10.1111/j.1365-2109 .2008.02105.x
Silici S, Kutluca S. 2005. Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J Ethnopharmacol., 99: 69-73. DOI: 10.1016/j.jep.2005.01.046.
Silici S, Koç AN, Mıstık S. 2007. Comparison of in vitro activities of antifungal drugs and propolis against yeasts isolated from patients with superficial mycoses. Ann Microbiol., 57 (2): 269-272. DOI: 10.1007/BF03175218.
Siwicki A, Studnicka M. 1987. The phagocytic ability of neutrophils and serum lysozyme activity in experimentally infected carp Cyprinus carpio L. J. Fish Biol., 31: 57-60. DOI: 10.1111/j.1095-8649.1987.tb05293.x.
Siwicki AK, Anderson DP, Rumsey GL. 1994. Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against frunculosis. Vet. Immunol. Immunop. 41: 125–129. DOI: 10.1016/0165-2427(94) 90062-0.
Tizard I. 1992. Veterinary Immunology an Introduction. Pennsylvania: W.B.Saunders Company.
Yonar ME, Yonar SM, Silici S. 2011. Protective effect of propolis against oxidative stress and immunosuppression induced by oxytetracycline in rainbow trout (Oncorhynchus mykiss, W.). Fish Shellfish Immun., 31: 318-325. DOI: 10.1016/j.fsi.2011.05.019.
Yonar ME. 2012. The effect of lycopene on oxytetracyclineinduced oxidative stress and immunosuppression in rainbow trout (Oncorhynchus mykiss, W.). Fish Shellfish Immun. 32: 994–1001. DOI: 10.1016/j.fsi.2012.02.012.