Gökkuşağı Alabalığı Yemlerine Probiyotik ve Antibiyotik İlavelerinin Balıkların Sağlık Karakteristikleri Üzerine Etkileri

Bu çalışmada yeme antibiyotik (amoksisilin+klavulanik asit) ve probiyotik ilavesinin gökkuşağı alabalıklarında (Oncorhynchus mykiss) bazı immünolojik ve serum biyokimyasal sağlık karakteristikleri üzerine etkilerinin araştırılması amaçlanmıştır. Yeme ilave edilen antibiyotik veya probiyotik katkılarının lizozim aktivitesi, myeloperoksidaz aktivitesi, serum total protein, albümin, globülin, trigliserit ve kolesterol değerleri üzerine herhangi bir etkisinin olmadığı belirlenmiştir. Antibiyotik ile beslenen balıkların serum glikoz düzeyleri kontrole göre önemli oranda artmıştır. Bununla birlikte antibiyotik ile beslenen balıkların GOT değeri kontrole göre önemli oranda azalmıştır. Ayrıca LDH ve ALP değerleri antibiyotik ve probiyotik ilavesi ile kontrole göre azaldığı belirlenmiştir. Bu çalışma, tek bir deneysel model olup farklı balık türlerinde ve farklı balık patojenlerinde karşı probiyotik kullanımı farklı dozlara veya zamana bağlı olarak değişebilir. Bu nedenle bu çalışma çeşitli kan parametrelerinin değerlendirilmesi açısından diğer çalışmalara ışık tutmaktadır.

The Effects of Dietary Probiotic and Antibiotic Supplementations on Health Characteristics of Rainbow Trout

The aim of this study was to assess the effect of dietary antibiotic (amoxycillin/clavulanic acid) and probiotic on the some immunological and serum biochemical health characteristics of rainbow trout (Oncorhynchus mykiss). Lysozyme activity, myeloperoxidase activity, total protein, albumin, globulin, triglyceride and cholesterol of rainbow trout was not affected by any of the dietary additives tested. The serum glucose in antibiotic group was significantly higher than the control group. However, the GOT level in antibiotic group was lower than the control group. In addition, compared with the control group, the LDH and ALP were deceased significantly in the antibiotic and probiotic treatments. This study is a single experimental model, and further investigations on the use of different concentrations of probiotic in different fish species and different fish pathogens are encouraged in terms of the evaluation of various blood parameters, since it is likely that the responses of fish seem to be subject to change in relation to different doses or time.

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  • Adam B, Ardıçoğlu Y. 2001. Klinik Biyokimya Analiz Metotları (1. Baskı). Atlas Kitapçılık, Ankara. 415 p. Babin PJ, Vernier JM. 1989. Plasma Lipoproteins in Fish. Journal of Lipid Research, 30: 467-489.
  • Başusta GA. 2005. Fish Hematology and Hematological Techniques. In: Karatas, M., Ed. Research Techniques in Fish Biology (in Turkish). Nobel Publications, Ankara, 275–300.
  • Brix O. 2002. The Physiology of Living in Water. In: Hart PJB, Reynolds JD, Ed. Handbook of Fish Biology and Fisheries. Blackwell Publishing. 71-96.
  • Brunt J, Newaj‐Fyzul A, Austin B. 2007. The development of probiotics for the control of multiple bacterial diseases of rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 30(10), 573-579.
  • Campbell TW. 2004. Clinical Chemistry of Fish and Amphibians. In: Thrall MA, Baker DC, Campbell TW, DeNicola D, Fettman MJ, Lassen ED, Rebar A, Weiser G. Eds. Veterinary Hematology and Clinical Chemistry. Lippincott Williams & Wilkins, Pennsylvania. 499–517.
  • Caruso D, Lusiastuti AM, Slembrouck J, Komarudin O, Legendre M. 2013. Traditional pharmacopeia in small scale freshwater fish farms in west Java, Indonesia: An ethno veterinary approach. Aquaculture, 416: 334-345.
  • Chesley LC. 1934. The concentrations of proteases, amylase, and lipase in certain marine fishes. Biological Bulletin, 66(2): 133-144.
  • Delbert M, Gatlin III, Peng Li T. 2007. Nucleotides. Dietary Supplements for the Health and Quality of Cultured Fish. Cabi, 12: 193-209.
  • Dunham RA. 2004. Aquaculture and Fisheries Biotechnology Genetic Approaches. CABI Publishing, UK. 372 p.
  • Ellis T, North B, Scott AP, Bromage NR, Porter M, Gadd D. 2002. The relationships between stocking density and welfare in farmed rainbow trout. Journal of Fish Biology, 61: 493-531.
  • Eya JC, Lovell RT. 1998. Effects of dietary phosphorus on resistance of channel catfish to Edwardsiella ictaluri challenge. Journal of Aquatic Animal Health, 10: 28–34.
  • Fange R. 1992. Fish Blood Cells. In: Hoar WS, Randall DJ,
  • Farrel AP. Eds. Fish Physiology: The Cardiovascular System, Part B volume XII. Academic Press, Inc., California. 2–46.
  • Francis G, Becker K. 2007. Plant Saponins. Dietary Supplements for the Health and Quality of Cultured Fish. Cabi, 11: 178-192.
  • Francis G, Makkar HPS, Becker K. 2002. Effects of cyclic and regular feeding of a Quillaja saponin supplemented diet on growth and metabolism of common carp (Cyprinus carpio L.). Fish Physiology and Biochemistry 24: 343–350.
  • Garcia LC, Minghetti M, Navarro I, Tocher DR. 2009. Molecular cloning, tissue expression and regulation of liver X receptor (LXR) transcription factors of Atlantic salmon (Salmo salar) and Rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology, Part B, 153: 81–88.
  • Gaw A, Murphy MJ, Cowan RA, Shepherd MJ. 1999. Clinical Biochemistry: An Illustrated Colour Text (2nd Ed.). Churchill Livingstone, 165 p.
  • Geurden I, Coutteau P, Sorgeloos P. 1997. Effect of a dietary phospholipid supplementation on growth and fatty acid composition of European sea bass (Dicentrarchus labrax L.) and Turbot (Scophthalmus maximus L.) juveniles from weaning onwards. Fish Physiology and Biochemistry, 16: 259-272.
  • Gunter G, Sulya LL, Box BE. 1961. Some evolutionary patterns in fishes' blood. The Biological Bulletin 121(2): 302–306.
  • Hadas E, Koven W, Sklan D, Tandler A. 2003. The effect of dietary Phosphatidylcholine on the assimilation and distribution of ingested free oleic acid (18:1n-9) in gilthead seabream (Sparus aurata) larvae. Aquaculture, 217: 577– 588.
  • Hart SD, Bharadwaj AS, Brown PB. 2010. Soybean lectins and trypsin inhibitors, but not oligosaccharides or the interactions of factors, impact weight gain of Rainbow trout (Oncorhynchus mykiss). Aquaculture, 306: 310–314.
  • Hong HA, Cutting SM. 2005. The use of bacterial spore formers as probiotics. FEMS microbiology reviews, 29: 813-835.
  • Janz DM, Weber LP. 2000. Endocrine system. In: Ostrander GK. Ed. The Laboratory Fish. Academic Press, London. 415–439.
  • Jonas A. 2002. Lipoprotein Structure. In: Vance DE. Vance JE. Eds. Biochemistry Lipids Lipoproteins amd Membranes (4th Ed.). Elsevier Scienc B.V. 483-504.
  • Karagül H, Altıntaş A, Fidancı UR, Sel T. 2000. Klinik Biyokimya (1. Baskı). Medisan Yayınevi, Ankara. 428 p.Liu CH, Chiu CH, Wang SW, Cheng W. 2012. Dietary administration of the probiotic, Bacillus subtilis E20, enhances the growth, innate immune responses, and disease resistance of the grouper, Epinephelus coioides. Fish & Shellfish Immunology, 33(4): 699-706.
  • Luo Z, Liu Y, Mai K, Tian L, Liu D, Tan X, Lin H. 2005. Effect of dietary lipid Level on growth performance, geed utilization and body composition of Grouper Epinephelus coioides juveniles fed isonitrogenous diets in floating netcages. Aquaculture international, 13: 257–269.
  • Magnadottir B. 2006. Innate immunity of fish (overview). Fish & Shellfish Immunology, 20(2): 137–151.
  • Mayes PA, Botham KM. 2003a. Metabolism of Acylglycerols & Sphingolipids. In: Murray RK, Granner DK, Mayes PA, Rodwell VW. Eds. Harper’s Illustrated Biochemistry (26th Ed.). McGraw-Hill. 197-204.
  • Mayes PA, Botham KM. 2003b. Cholesterol Synthesis, Transport, & Excretion In: Murray RK, Granner DK, Mayes PA, Rodwell VW. Eds. Harper’s Illustrated Biochemistry (26th Ed.). McGraw-Hill. 219-230.
  • McDonald DG, Milligan CL. 1992. Chemical Properties of the Blood. In: Hoar WS, Randall DJ, Farrel AP. Eds. Fish Physiology: The Cardiovascular System, Part B volume XII. Academic Press, Inc., California. 56-113.
  • Mehmetoğlu İ. 2007. Klinik Biyokimya Laboratuvarı El Kitabı (4. Baskı). Nobel Tıp Kitapevleri, İstanbul. 409 p.
  • Messager JL, Stéphan G, Quentel C, Laurcncin BF. 1992. Effects of Dietary Oxidized Fish Oil and Antioxidant Deficiency on Histopathology, Haematology, Tissue and Plasma Biochemistry of Sea Bass Dicentrarchus labrax. Aquat. Living Resour., 5(3): 205–214.
  • Mohapatra S, Chakraborty T, Kumar V, DeBoeck G, Mohanta KN. 2013. Aquaculture and stress management: a review of probiotic intervention. Journal of Animal Physiology and Animal Nutrition, 97: 405-430.
  • Moon TW. 2001. Glucose intolerance in teleost fish: fact or fiction?. Comparative Biochemistry and Physiology Part B, 129: 243-249.
  • Morgan JD, Iwama GK. 1997. Measurements of Stressed States in the Field. In: Iwama GK, Pickering AD, Sumpter JP, Schreck CB. Eds. Fish Stress and Health in Aquaculture. Cambridge University Press, Cambridge. 247-270.
  • Nakagawa H. 2007. Chitin. Dietary Supplements for the Health and Quality of Cultured Fish. Cabi, 10: 168-177.
  • Nakagawa H, Montgomery L. 2007. Algae. Dietary Supplements for the Health and Quality of Cultured Fish. Cabi, 9: 133-167.
  • Nayak SK. 2010. Probiotics and immunity: a fish perspective. Fish and Shellfish Immunology, 29: 2-14.
  • Newaj‐Fyzul A, Adesiyun AA, Mutani A, Ramsubhag A, Brunt J, Austin B. 2007. Bacillus subtilis AB1 controls aeromonas infection in Rainbow trout (Oncorhynchus mykiss Walbaum). Journal of Applied Microbiology, 103: 1699- 1706.
  • Ng WK, Koh CB. 2017. The utilization and mode of action of organic acids in the feeds of cultured aquatic animals. Reviews in Aquaculture, 9: 342-368
  • North BP, Turnbull JF, Ellis T, Porter MJ, Migaud H, Bron J, Bromage NR. 2006. The impact of stocking density on the welfare of Rainbow trout (Oncorhynchus mykiss). Aquaculture, 255: 466-479.
  • Peres H, Goncalves P, Teles AO. 1999. Glucose tolerance in gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax).Aquaculture, 179: 415–423.
  • Park Y, Lee S, Hong J, Kim D, Moniruzzaman M, Bai SC. 2017. Use of probiotics to enhance growth, stimulate immunity and confer disease resistance to Aeromonas salmonicida in rainbow trout (Oncorhynchus mykiss). Aquaculture Research, 48(6): 2672-2682.
  • Pérez‐Sánchez T, Ruiz‐Zarzuela I, Blas I, Balcazar JL. 2014. Probiotics in aquaculture: a current assessment. Reviews in Aquaculture, 6: 133-146.
  • Satchell GH. 1991. Physiology and Form of Fish Circulation. Cambridge University Press, Cambridge. 235 p.
  • Serrano PH. 2005. Responsible use of antibiotics in aquaculture (No. 469). Food & Agriculture Org
  • Sheridan MA. 1988. Lipid Dynamics in Fish: Aspects of Absorption, Transportation, Deposition and Mobilization. Comp. Biochem. Physiol., 90: 679-690.
  • Stoskopf M. 1993. Fish Medicine (1st Ed.). Saunders Company, Philadelphia. 882 p.
  • Timur M. 2006. Balık Fizyolojisi (1. Baskı). Nobel Yayın Dağıtım, Ankara. 192 p.
  • Tramati C, Savona B, Mazzola A. 2005. A Study of the pattern of dgestive enzymes in Diplodus puntazzo (Cetti, 1777) (Osteichthyes, Sparidae): evidence for the definition of nutritional protocols. Aquaculture International, 13: 89–95.
  • Ulukaya E. 1998. Klinik Biyokimya. Melisa Matbaacılık, İstanbul. 343 p.
  • Wang YB, Li JR, Lin J. 2008. Probiotics in aquaculture: challenges and outlook. Aquaculture, 281: 1-4.
  • Wiegertjes GF, Stet RJ, Parmentier HK, van Muiswinkel WB. 1996. Immunogenetics of disease resistance in fish: a comparative approach. Developmental & Comparative Immunology, 20(6): 365-381.
  • Wilson RP. 1994. Utilization of dietary carbohydrate by fish. Aquaculture, 124: 67-80.
  • Yılmaz S, Ergün S. 2012. Effects of garlic and ginger oils on hematological and biochemical variables of sea bass Dicentrarchus labrax. Journal of Aquatic Animal Health, 24: 219-224.
  • Yılmaz S, Ergün S. 2014. Dietary supplementation with allspice Pimenta dioica reduces the occurrence of streptococcal disease during first feeding of Mozambique Tilapia fry. Journal of Aquatic Animal Health, 26: 144-148.
  • Yilmaz S. 2017. The effect of dietary cinnamic acid or Bacillus subtilis on growth performance and immunological parameters in rainbow trout, department of aquaculture, Çanakkale Onsekiz Mart University, Graduate School of Natural and Applied Sciences, Türkiye, 2017, p. 210.
  • Yılmaz S, Ergün S, Çelik EŞ. 2012. Effects of herbal supplements on growth performance of sea bass (Dicentrarchus labrax): change in body composition and some blood parameters. Journal of BioScience and Biotechnology, 1: 217-222.
  • Yılmaz S, Ergün S, Çelik EŞ. 2013. Effect of dietary herbal supplements on some physiological conditions of sea bass Dicentrarchus labrax. Journal of Aquatic Animal Health, 25: 98-103.
  • Yılmaz S, Ergün S, Çelik EŞ. 2016. Effect of dietary spice supplementations on welfare status of sea bass Dicentrarchus labrax L. Proceedings of the National Academy of Sciences India Section B: Biological Sciences, 86: 229-237.
  • Yılmaz S, Ergün S, Kaya H, Gürkan M. 2014. Influence of Tribulus terrestris extract on the survival and histopathology of Oreochromis mossambicus (Peters 1852) fry before and after Streptococcus iniae infection. Journal of Applied Ichthyology, 30: 994-1000.
  • Zilberg D, Tal A, Froyman N, Abutbul S, Dudai N, Goldhirsh AG. 2010. Dried leaves of Rosmarinus officinalis as a treatment for smtreptococcosis in tilapia. Journal of Fish Diseases, 33(4): 361-369.
Türk Tarım - Gıda Bilim ve Teknoloji dergisi-Cover
  • ISSN: 2148-127X
  • Yayın Aralığı: Aylık
  • Başlangıç: 2013
  • Yayıncı: Turkish Science and Technology Publishing (TURSTEP)
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