ELİF CANPOLAT, Müzeyyen Müge DOĞANER, SİBEL DERVİŞ, ÇİĞDEM ULUBAŞ SERÇE

Antifungal Activity of Some Lactic Acid Bacteria Against Several Soilborne Fungal Pathogens Isolated from Strawberry Plants

Developing as an alternative plant disease control method by using beneficial microorganisms and their metabolites has gained so much importance in recent years. In this study, the possibilities of using microorganisms which have potential antimicrobial effects on controlling soil-borne fungi at strawberry production were investigated. Effects of different lactic acid bacteria (LAB) strains on the development of several soil-borne fungi were studied in vitro and in vivo. LAB were screened for antifungal activity by using cell free supernatant against Fusarium sp., Rhizoctonia sp., Macrophomina sp., Botrytis sp., Phtopythium sp., Cylindrocarpon sp. and Pestalotiopsis sp. Cell free supernatant of LAB isolates showed promising antifungal activity. In vitro effective strains of LAB were tested in pot experiments to search their effects on disease development and plant growth. While the antifungal effects of all LAB strains tested in vitro experiments exhibited promising results, in vivo experiments revealed similar effects on different fungi genera.

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Aksoy HM. 2006. Biological control of soil-borne fungal pathogens by fluorescent Pseudomonads. J. Agricultural Faculty of OMU, 21(3): 364-369.

Alaniz S, Leon M, García-Jiménez J, Aba-Campos P, Armengol J. 2007. Characterization of Cylindrocarpon species associated with black-foot disease of grapevine in Spain. Plant Disease, 91: 1187–1193. DOI:10.1094/ PDIS-91-9- 1187.

Benlioğlu K. 2012. Plant Pathogenic Bacteria, Plant Protection Lecture notes.

Bhunia AK, Johnson MC, Ray B. 1988. Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidilactici. Journal of Applied Bacteriology, 65: 261-268. https://doi.org/10.1111/j.1365- 2672.1988.tb01893.x

Dykes GA, Cloete TE, Holy A. 1994. Identification of Leuconostoc species associated with the spoilage of vacuum-packaged Vienna sausage by DNA-DNA hybridization. International Journal of Food Microbiology, 11: 271–274. DOI10.1006/fmic.1994.1030

El-Mabrok ASW, Hassan Z, Mokhtar AH, Hussain KMA, Kahar FKSBA. 2012. Screening of Lactic acid bacteria as biocontrol against (Colletotrichum capsici) on chilli bangi. Research Journal of Applied Sciences, 7 (9-12): 466-473. DOI: 10.3923/rjasci.2012.466.473.

Hamed HA, Moustafa YA, Abdel-Aziz SM. 2011. In vivo efficacy of Lactic Acid Bacteria in biological control against Fusarium oxysporum for protection of tomato plant. Life Science Journal, 8 (4): 462-468.

Husain A. Hassan Z. Huda-Faujan N, Lani MN. 2017. Antifungal activity of Lactic Acid Bacteria isolated from soil rhizosphere on Fusarium species infected chilli seeds. American Scientific Research Journal for Engineering, Technology, and Sciences, 29 (1): 182-202.

Johan S, Jesper M. 2005. Antifungal lactic acid bacteria as biopreservatives. Trends Food Science Technology, 1: 70- 78. DOI:10.1016/j.tifs.2004.02.014

Kim WJ. 1993. Bacteriocins of lactic acid bacteria: their potentials as food preservatives. Food Reviews International, 9: 299-313.

Lutz MP, Michel V, Martinez C, Camps C. 2012. Lactic acid bacteria as biocontrol agents of soil-borne pathogens, IOBCWPRS Bulletin, 78: 285-288, 2012.

Maas JL. 1998. Compendium of Strawberry Diseases. 2nd ed. St. Paul, MN, USA: APS Press.

Ouwehand A. 1998. In Lactic acid bacteria Microbiology and Functional Aspects. 2nd ed. NY, USA: CRC Press.

Trias R, Baneras L, Montesinos E, Badosa E. 2008. Lactic acid bacteria from fresh fruit and vegetables as biocontrol agents of phytopathogenic bacteria and fungi. Int Microbial., 11: 231-236. DOI: 10.2436/20.1501.01.66.

Turhan G, Turhan K. 1989. Supression of damping off on pepper caused by Phythium ultimum Trow and Rhizoctonia solani Kühn. by Some New Antagonists in Comparison with Trichoderma harzianum Rifai. J Phytopathology., 126: 175– 182.

Vestberg M, Kukkonen S, Saari K, Parikka P, Huttunen J, Tainio L, Devos N, Weekers F, Kevers C, Thonart P, Lemoine MC, Cordiwe C, Alabouvette C, Gianinazzi S. 2004. Microbial inoculation for improving the growth and health of micropropagated strawberry. Applied Soil Ecology, 27: 243-258. DOI:10.1016/j.apsoil.2004.05.006

Zebboudj N, Yezli W, Hamini-Kadar N, Kihal M, Henni JE. 2014. Antifungal activity of lactic acid bacteria against Fusarium oxysporum f. sp. albedinis isolated from diseased date palm in South Algeria. International Journal of Biosciences, 5 (9): 99-106.