Esra ŞENTÜRK, Pınar ŞANLIBABA, Sema YAKIT

Geleneksel Peynirlerden İzole Edilen Enterococcus Türlerinin Antilisterial Aktivitesi

Bu çalışmada, 312 adet Enterococcus suşunun Listeria monocytogenes’e karşı antimikrobiyel aktivitesinin saptanması amaçlanmıştır. Antimikrobiyel aktivite agar spot test ve kuyu difüzyon testleri ile tanımlanmıştır. Agar spot test sonucunda toplamda 201 enterokokkal suş, L. monocytogenes gelişimini inhibe etmiştir. Kuyu difüzyon testi sonucunda sadece 44 suş gıda kaynaklı patojene karşı antimikrobiyel aktivite göstermiştir. Taraması yapılan 44 suş içinden, 6 tanesi (%2,99) indikatör suşa karşı yüksek antimikrobiyel etki göstermiştir. Proteinaz K, tripsin ve pepsin uygulamaları sonucunda, 6 E. faecium suşunun antilisterial aktivitesi kaybolmuştur. Bu suşlardaki antimikrobiyel aktivitenin doğası protein veya peptit yapısındaki antimikrobiyel bileşiklerden kaynaklanabilir. E. faecium suşları, E. faecalis suşlarına oranla indikatör suşlara karşı daha fazla aktivite göstermiştir.

Antilisterial Activity by Enterococcus Species Isolated from Traditional Cheeses

In this study, it was aimed to detect the antimicrobial activity of 312 Enterococcus species against Listeria monocytogenes. Antimicrobial activity was detected by agar spot and well diffusion assay. A total of 201 enterococcal strains inhibited the growth of L. monocytogenes strains based on the agar spot test. Only 44 strains showed antimicrobial activity against L. monocytogenes strains using agar well diffusion assay. Of the 44 enterococcal strains screened, 6 E. faecium (2.99%) strains had a high antimicrobial effect against indicator L. monocytogenes strains. The antilisterial activity of 6 E. faecium strains had lost after treatment of proteinase K, trypsin and pepsin. The antimicrobial compounds of these strains could be a protein or peptides nature. E. faecium strains were more active against L. monocytogenes than E. faecalis strains.

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Akkoç N, Ghamat, A, Akçelik, M. 2011. Optimization of bacteriocin production of Lactococcus lactis subsp. lactic MA23, a strain isolated from Boza. International Journal of Dairy Technology, 64(3): 425-432. https://doi.org/10.1111/ j.1471-0307.2011.00671.x
Anandani JH, Khan ZH. 2014. Isolation, partial purification and biochemical characterization of enterocin producing enterococci. International Journal of Advanced Research, 4(2): 18-20. DOI: https://doi.org/10.36106/ijar
Anonymous 2020. Available from, https://www.ncbi.nlm.nih.gov/ Taxonomy/Browser/wwwtax.cgi [Accessed April 09, 2020]
Arachchi GJG, Cruz CD, Dias-Wanigaselera BM, McIntrye L, Billington C, Hudson A, Flint SH, Mutukumira AN. 2015. Host range and invitro lysis of Listeria monocytogenes seafood isolates by bacteriophages. Food Science and Technology International, 20: 591-603. https://dx.doi.org/ 10.1177/1082013213497211
Banos A, Garcia Lopez JD, Nunez C, Martinez-Bueno M, Maqueda M, Valdivia E. 2016. Biocontrol of Listeria monocytogenes in fish by enterocin AS-48 and Listeria lytic bacteriophage P100. LWT-Food Science and Technology, 66: 672-677. https://doi.org/10.1016/j.lwt.2015.11.025
Bigot B, Lee WJ, McIntyre L, Wilson T, Hudson JA, Billington C, Heinemann JA. 2011. Control of Listeria monocytogenes growth in a ready-to eat poultry product using a bacteriophage. Food Microbiology, 28: 1448-1452. https://doi.org/10.1016/j.fm.2011.07.001
Comerlato CB, Bublotz JR, Santestevan NA, da Motta AS, Frazzon APG. 2016. Antimicrobial compounds produced by Enterococcus spp. Isolates from fecal samples of wild South American fur seals. Journal of Microbiology and Antimicrobials, 8(3), 14-21. https://dx.doi.org/10.5897/ JMA2015.0360
Dündar H, Atakay M, Çelikbıçak Ö, Salih B, Bozoğlu F. 2015. Comparision of two methods for purification of enterocin B, a bacteriocin produced by Enterococcus faecium W3. Preparative Biochemistry and Biotechnology, 45(8): 796-809. https://doi.org/10.1080/10826068.2014.958165
El-Ghaish S, sfaxi IH, Ahmadova A, Choiset Y, Rabesona H, Sitohy M, Haertle T, Chobert JM. 2011. Characterization of two safe Enterococcus strains producing enterocins isolated from Egyptian dairy products. Beneficial Microbes, 2(1): 15-27. https://dx.doi.org/ 10.3920/BM2011.0001
Favaro L, Basaglia M, Casella S, Hue I, Dousset X, de Melo Franco BDG, Todorov SD. 2014. Bacteriocinogenic potential and safety evaluation of non-starter Enterococcus faecium strains isolated from homemade white brine cheese. Food Microbiology, 38: 228-239 https://dx.doi.org/10.1016/ j.fm.2013.09.008
Gaaloul N, Braiek OB, Hani K, Volski A, Chikindas ML, Ghrairi T. 2014. Isolation and characterization of large spectrum and multiple bacteriocin-producing Enterococcus faecium strain from raw bovine milk. Journal of Applied Microbiology, 118, 343-355. https://dx.doi.org/ 10.1111/jam.12699
Galvez A, Abriouel H, Benomar N, Kucas R. 2010. Microbial antagonists to foodborne pathogens and biocontrol. Current Opinion in Biotechnology, 21: 142-148. https://doi.org/10.1016/ j.copbio.2010.01.005
Hosseini SV, Arlindo S, Böhme K, Fernandez-No C, Calo-Mata P, Barros-Velazquez J. 2009. Molecular and probiotic characterization of bacteriocin-producing Enterococcus faecium strains isolated from nonfermented animal foods. Journal of Applied Microbiology, 107,1392-1403. https://dx.doi.org/ 10.1111/j.1365-2672.2009. 04327.x
Huang E, Zhang L, Chung YK, Zheng Z, Yousef AE. 2013. Characterization and application of enterocin RM6, a bacteriocin from Enterococcus faecalis. BioMed Research International, Volume 2013, Article ID 206917, 6 pages https://dx.doi.org/10.1155/2013/206917
Jurkovic D, Krizkova L, Dusinsky R, Belicova A, Ajka M, Krajcovic J, Ebringer L. 2006. Identification and characterization of enterococci from bryndza cheese. Letters in Applied Microbiology, 42, 553-559. https://dx.doi.org /10.1111/j.1472-765X.2006.01918.x
Kurpas M, Wieczorek K, Osek J. 2018. Ready-to-eat meat products as a source of Listeria monocytogenes. Journal of Veterinary Research, 61, 49-55. https://dx.doi.org/ 10.1515/jvetres-2018-0007
Lee S, Kim MG, Lee HS, Heo S, Kwon M, Kim GB. 2017. Isolation and characterization of Listeria phages for control of growth of Listeria monocytogenes in milk. Korean Journal for Food Science of Animal Resources, 37(2): 320-328. https://dx.doi.org/10.5851%2Fkosfa.2017.37.2.320
Ohmomo S, Murata S, Katayama N, Nitisinprasart S, Kobayashi M, Nakajima T. 2000. Purification and some characteristics of enterocin ON-157, a bacteriocin produced by Enterococcus faecium NIAI 157. Journal of Applied Microbiology, 88, 81-89. https://dx.doi.org/ 10.1046/j.1365-2672.2000.00866.x
Omar NB, Abriovel H, Lucas R, Martinez-Canamero M, Guyot JP, Galvez A. 2006. Isolation of bacteriocinogenic Lactobacillus plantarum strains from ben saalga, a traditional fermented gruel from Burkina Faso. International Journal of Food Microbiology, 112; 44-50. https://dx.doi.org/ 10.1016/j.ijfoodmicro.2006.06.014
Renye JA, Somkuti GA, Paul M, Hekken DL. 2009. Characterization of antilisterial bacteriocins produced by Enterococcus faecium and Enterococcus durans isolates from Hispanic-style cheeses. Journal of Industrial Microbiology and Biotechnology, 36, 261-268. https://dx.doi.org/ 10.1007/s10295-008-0494-7
Ruiz AG, Reguena T, Pelaez C, Bartolome B, Moreno-Arribas MV, Martinez-Cuesta MC. 2013. Antimicrobial activity of lacticin 3147 against oenological lactic acid bacteria: Combined effect with other antimicrobial agents. Food Control, 32(2): 477-483. https://doi.org/10.1016/j.foodcont.2013.01.027
Schillinger U, Lucke FK. 1989. Antibacterial activity of Lactobacillus sake isolated from meat. Applied and Environmental Microbiology, 55: 1901-1906.
Schittler L, Perin LM, Marques JDL, Lando V, Todorov SD, Nero LA, da Silva WP. 2019. Isolation of Enterococcus faecium, characterization of its antimicrobial metabolites and viability in probiotic Minas Frescal cheese. Journal of Food Science and Technology, 56(11): 5128-5137 https://doi.org/10.1007/ s13197-019-03985-2
Soni KA, Shen Q, Nannapaneni R. 2014. Reduction of Listeria monocytogenes in cold-smoked salmon by bacteriophage P100, nisin and lauric arginate, singly or in combinations. International Journal of Food Science and Technology, 49, 1918-1924 https://dx.doi.org/10.1111/ijfs.12581
Şanlıbaba P, Şentürk E. 2018. Prevalence, Characterization and Antibiotic Resistance of Enterococci from Traditional Cheeses in Turkey. International Journal of Food Properties, 21(1): 1955-1963, https://dx.doi.org/10.1080/10942912.2018.1489413
Şanlıbaba P, Tezel BU, Çakmak GA. 2018. Prevalence and Antibiotic Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Foods in Turkey. Hindawi Journal of Food Quality, Article ID 7693782, 9 pages. https://dx.doi.org/10.1155/2018/7693782
Tagg JR, McGiven AR. 1971. Assay systems for bacteriocins. Applied and Environmental Microbiology, 21, 943-947.
Terzic-Vidojevic A, Mihajlovic S, Uzelac N, Golic G, Fira D, Kojic M, Topisirovic L. 2014. Identification and characterization of lactic acid bacteria isolated from artisanal white brined Golija cows’ milk cheeses. Archives of Biological Sciences, 66(1): 179-192. https://dx.doi.org/ 10.2298/ABS1401179T
Tezel BU. 2019. Preliminary evaluation af anti-listerial bacteriocin-like peptide produced by Enterococcus lactis PMD74 isolated from Ezine cheese. Turkish Journal of Agriculture-Food Science and Technology, 7(5): 802-808. https://dx.doi.org/10.24925/turjaf.v7i5.802-808.2489
Toğay SÖ, Temiz A, Çelebi A, Açık L, Yalçın SS. 2014. Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples. Turkish Journal of Biology, 38, 357-364. https://dx.doi.org/10.3906/biy-1311-34
Toğay SÖ, Ay M, Altunatmaz SS, Aksu FY, Tınaztepe ÖE, İssa G, Büyükünal SK. 2016. Antimicrobial activity potential of Enterococcus spp. isolated from some traditional Turkish cheeses. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(5): 765-770. https://dx.doi.org/10.9775/kvfd.2016.15369
Trivedi K, Sedmikova R, Karpiskova R. 2012. Bacteriocin activity of enterococci and presence of genes related to pathogenesis. Czech Journal of Food Science, 30(4): 330-335.https://dx.doi.org/10.17221/181/2011-CJFS
Turhan EU, Erginkaya Z, Turemiş AB, Terkuran M. 2018. Antibacterial effect of bacteriocinogenic enterococci from different sources on Listeria monocytogenes. Tarım Bilimleri Dergisi, 26: 1-7, https://doi.org/10.15832/ankutbd.448475