AYŞE GÜL NASIRCILAR, KAMİLE ULUKAPI, Zehra KURT

Effects of Salicylic Acid on Germination and Vegetative Growth Properties of Radish (Raphanus sativus L.) Cultivars Grown under Drought Stress Conditions

In this study, firstly different PEG 6000 concentrations (%5, %10, %15, %20) were applied to four different radish cultivars (black, white, big red, little radish) to determine germination and vegetative growth parameters under drought conditions. As germination parameter; germination percentage (%), germination time and vigor index were determined. After germination, number of leaves, shoot length (cm), root length (cm), leaf width (cm) leaf length (cm), stem diameter (mm), plant fresh and dry weights (g) were measured in germinated plants. Vegetative growth parameters decreased in %15 PEG6000 for white, black and red little radish cultivars and in %20 for big red radish cultivar, and these doses were determined as the threshold value for salicylic acid applications. In second step, in order to increase the drought tolerance of the cultivars, different doses of salicylic acid (0.25, 0.50, 0.75, 1.00 mM) were applied in addition to PEG6000 application. The effect of different doses of salicylic acid on germination and vegetative growth parameters of radish seeds under drought conditions varied depending on the cultivar, and it was determined that it caused a stimulatory effect on germination of the cultivars. Except for the large red radish cv, it has a positive effect on plant growth in general. It was found that SA doses applied in this cultivar had an inhibitory effect. 0.50 mM salicylic acid in black radish; 0.25 mM salicylic acid applications in white and little red radish provided positive results in terms of improving vegetative parameters at %15 PEG6000 concentration.

Kuraklık Stresi Koşullarında Dışarıdan Uygulanan Salisilik Asitin Turp (Raphanus sativus L.) Çeşitlerinin Çimlenme ve Vejetatif Büyüme Özellikleri Üzerine Etkisi

Bu çalışmada ilk olarak dört farklı turp çeşidinin (siyah, beyaz, iri kırmızı, kırmızı fındık) farklı PEG6000 (%5, %10, %15, %20) konsantrasyonları uygulanarak oluşturulan kuraklık koşullarında çimlenme ve vejetatif gelişim parametreleri değerlendirilmiştir. Çimlenme parametresi olarak; çimlenme yüzdesi (%), çimlenme süresi ve vigor indeksi belirlenmiştir. Bitkilerde vejetatif gelişim parametresi olarak; yaprak sayısı (adet), sürgün boyu (cm), kök uzunluğu (cm), yaprak genişliği (cm), yaprak uzunluğu (cm), gövde çapı (mm), bitki yaş ve kuru ağırlıkları (g) ölçülmüştür. Beyaz, siyah ve kırmızı fındık turp çeşitlerinde %15, kırmızı iri turp çeşidinde ise %20 PEG uygulamalarında vegetatif gelişim parametreleri belirgin bir şekilde düşüş göstermiş olup, bu dozlar salisilik asit uygulamaları için eşik değer olarak belirlenmiştir. İkinci aşamada, çeşitlerin kuraklığa toleransını arttırmak amacıyla PEG6000 içeren ortamlara farklı dozlarda salisilik asit (0,25, 0,50, 0,75, 1,00 mM) uygulanmıştır. Salisilik asitin farklı dozlarının, kuraklık koşullarında turp tohumlarının çimlenmesi ve vejetatif gelişim parametreleri üzerine etkisi, çeşide bağlı olarak değişiklik göstermiş olup, çimlenmeyi teşvik edici etki yaptığı belirlenmiştir. İri kırmızı turp çeşidi hariç, bitki gelişimi üzerinde de genel anlamda olumlu etki yapmış, bu çeşitte uygulanan SA dozlarının inhibe edici etki yaptığı tespit edilmiştir. Siyah turp çeşidinde eşik değeri olarak belirlenen %15’lik PEG6000 konsantrasyonunda 0,50 mM salisilik asit uygulaması; beyaz ve kırmızı fındık turp çeşitlerinde ise eşik değeri olarak belirlenen %15’lik PEG6000 konsantrasyonunda 0,25 mM salisilik asit uygulaması vegetatif parametrelerinin iyileştirilmesi açısından oldukça iyi sonuç vermiştir.

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