Cotoneaster Transcaucasicus Pojark. Determination of Bioactive Component Amounts and Antioxidant Activities in Fruit Extracts

In this study, the total phenolic and flavonoid contents and antioxidant activities of hexane and methanol extracts of Cotoneaster transcaucasicus Pojark. fruits in the Rosaceae family were investigated. Using the Folin-Ciocaltaeu method, the amount of phenolic substances was determined as 50.34 ± 2.70 mg of GAE/g in methanol extract and 38.06 ± 1.32 mg of GAE/g in hexane extract. The total amount of flavonoids was calculated as 52.01 ± 3.78 mg of QE/g in the methanol extract and 60.54 ± 6.82 mg of QE/g in the hexane extract, using the aluminum nitrate method. Antioxidant activities of C. transcaucasicus hexane and methanol extracts were evaluated by DPPH and FRAP methods. IC50 values of the extracts were calculated as 0.411 mg/mL for hexane extract and 0.237 mg/mL for methanol extract. In addition, it was determined that the reducing antioxidant power of Fe3+ -Fe2+ was higher in hexane extract. As a result, it has been observed that the phenolic and flavonoid content and antioxidant activities of C. transcaucasicus fruits are high. This study is the first study in C. transcaucasicus species and it is thought that it will lead the future studies.

Cotoneaster transcaucasicus Pojark. Meyve Ekstraklarında Biyoaktif Bileşen Miktarlarının ve Antioksidan Aktivitelerinin Belirlenmesi

Bu çalışmada Rosaceae familyasında yer alan Cotoneaster transcaucasicus Pojark meyvelerinin hekzan ve metanol ekstraklarının toplam fenolik madde ve flavonoid madde içerikleri ile antioksidan aktiviteleri araştırılmıştır. Folin-Ciocaltaeu metodu kullanılarak fenolik madde miktarları metanol ekstraktında 50,34±2,70 mg GAE/g ve hekzan ekstraktında 38,06±1,32 mg GAE/g olarak belirlendi. Toplam flavonoid madde miktarı ise alüminyum nitrat yöntemi kullanılarak metanol ekstraktında 52,01±3,78 mg KE/g ve hekzan ekstraktında ise 60,54±6,82 mg KE/g olarak hesaplandı. C. transcaucasicus hekzan ve metanol ekstraklarının antioksidan aktiviteleri, DPPH ve FRAP yöntemleri ile değerlendirildi. Ekstrakların IC50 değerleri hekzan ekstraktı için 0,411mg/mL, metanol ekstraktı için 0,237 mg/ mL olarak hesaplandı. Ayrıca Fe3+ -Fe2+ indirgeyici antioksidan gücünün hekzan ekstraktında daha fazla olduğu belirlendi. Sonuç olarak C. transcaucasicus meyvelerinin fenolik ve flavonoid madde miktarlarının ve antioksidan aktivitelerinin yüksek olduğu görülmüştür. Bu çalışma C. transcaucasicus türünde yapılan ilk çalışma niteliğinde olup bundan sonra yapılacak olan çalışmalara öncülük edeceği düşünülmektedir.

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Akkemik U. 2018. Türkiye’nin Doğal Egzotik Ağaç ve Çalıları, s.501.

Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181: 1199-1200.

Browicz K. 1972. Cotoneaster. In P. H. Davis (Ed.), Flora of Turkey and the East Aegean Islands. Edingburg University Press, 129-132.

Cao G, Sofic E, Prior RL. 1996. Antioxidant capacity of tea and common vegetables. Journal of Agricultural and Food Chemistry, 44: 3426–3431.

Česonienė L, Daubaras R, Venclovienė J, Viškelis P. 2010. Biochemical and agrobiological diversity of Viburnum opulus genotypes. Central European Journal of Biology, 5: 864–871.

Ceylan Ş, Saral Ö, Özcan M, Harşıt B. 2017. Yaban mersininin (Vaccinium myrtillus L.) farklı çözücü ekstraktlarındaki antioksidan ve antimikrobiyal aktivitelerinin belirlenmesi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 18: 21-27.

Chai WM, Chen CM, Gao YS, Feng HL, Ding YM, Shi Y, Zhou HT, Chen QX. 2014. Structural Analysis of Proanthocyanidins Isolated from Fruit Stone of Chinese Hawthorn with Potent Antityrosinase and Antioxidant. J. Agric. Food Chem, 62: 123-129.

Çakılcıoğlu U, Khatun S, Türkoğlu I, Hayta S. 2011. Ethnopharmacological survey of medicinal plants in Maden (Elazig-Turkey). J Ethnopharmocol, 137: 469-486.

Davis PH. 1972. Türkiye Florası ve Doğu Ege Adaları, Edinburgh, 4.

Demir T, Akpınar Ö, Kara H, Güngör H. 2019. Nar (Punica granatum L.) kabuğunun in vitro antidiyabetik, antienflamatuar, sitotoksik, antioksidan ve antimikrobiyal aktivitesi. Akademik Gıda, 17(1): 61-71.

Demir T, Akpınar Ö. 2020. Biological activities of phytochemicals in Plants. Turkish Journal of AgricultureFood Science and Technology, 8(8):1734-1746.

El-Mousallamy AM, Hussein SA, Merfort I, Nawwar MA. 2020. Unusual phenolic glycosides from Cotoneaster orbicularis. Phytochemistry, 53: 699-704.

Erlund I, Koli R, Alfthan G, Marniemi J, Puukka P, Mustonen P, Mattila P, Jula A. 2008. Favorable effects of berry consumption on platelet function, blood pressure, and HDL cholesterol. The American Journal of Clinical Nutrition, 87(2): 323-331.

Foo LY, Porter LJ. 1981. The structure of tannins of some edible fruits. Journal Science Food Agricaltural, 32; 711-716.

Kicel A, Michel P, Owczarek A, Marchelak A, Żyżelewicz D, Budryn G, Oracz J, Olszewska MA. 2016. Phenolic profile and antioxidant potential of leaves from selected Cotoneaster Medik. Species. Molecules, 21(6): 688.

Kicel A. 2020. An Overview of the Genus Cotoneaster (Rosaceae): Phytochemistry, Biological Activity, and Toxicology. Antioxidants, 9: 1002.

Macit M. 2018. Rosa canina L. ve Rosa pimpinellifolia L. Köklerindeki Fenolik Bileşiklerin Miktarı ve Bioyararlılığının Tespiti. İstanbul Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Yüksek Lisans Tezi, İstanbul, 92s.

Mahapatra AK, Panda PC. 2012. Wild edible fruit diversity and its significance in the livelihood of indigenous tribals: Evidence from eastern India. Food Security, 4(2): 219-234.

Mahmutović-Dizdarević I, Dizdar M, Čulum D, Vidic D, Dahija S, Jerković-Mujkić A, Bešta-Gajević R. 2020. Phenolic Composition, Antioxidant and Antimicrobial Activity of Cotoneaster Medik. Species from Bosnia and Herzegovina, Bulletin of the Chemists and Technologists of Bosnia and Herzegovina, 54: 1-6.

Mohamed SA, Sokkar NM, El-Gindi O, Zeinab Y, Alfishawy IM. 2012. Phytoconstituents investigation, anti-diabetic and antidyslipidemic activities of Cotoneaster horizontalis Decne cultivated in Egypt. Life Science Journal, 9: 394-403.

Mohammed FS, Pehlivan M, Sevindik M. 2019. Antioxidant, antibacterial and antifungal activities of different extracts of Silybum marianum collected from Duhok (Iraq). International Journal of Secondary Metabolite, 6(4): 317-322.

Mohammed FS, Şabik AE, Sevindik E, Pehlivan M, Sevindik M. 2020. Determination of Antioxidant and Oxidant Potentials of Thymbra spicata Collected from Duhok-Iraq. Turkish Journal of Agriculture-Food Science and Technology, 8(5): 1171-1173.

Mohammed FS, Kına E, Sevindik M, Dogan M, Pehlivan M. 2021. Datura stramonium (Solanaceae): Antioxidant and Antimicrobial Potentials. Turkish Journal of AgricultureFood Science and Technology, 9(4): 818-821.

Moreno MIN, Isla MI, Sampietro AR, Vattueno MA. 2000. Comparison of the free radical scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology, 71: 109-114.

Oyaizu M. 1986. Study on products of Browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr, 44: 307-315.

Ozgen U, Kaya Y, Houghton P. 2012. Folk medicines in the villages of Ilıca District (Erzurum-Turkey). Turk. J. Biol, 36: 93-106.

Pehlivan M, Mohammed FS, Sevindik M, Akgul H. 2018. Antioxidant and oxidant potential of Rosa canina. Eurasian Journal of Forest Science, 6(4): 22-25.

Polat R, Çakılcıoğlu U, Satil F. 2013. Solhan'da (Bingöl-Türkiye) tıbbi bitkilerin geleneksel kullanımları. J. Ethnopharmocol, 148: 951-963.

Sevindik M, Akgul H, Pehlivan M, Selamoglu Z. 2017. Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresen Environ Bull, 26(7): 4757-4763.

Slinkard K, Singleton VL. 1977. Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28: 49-55.

Sokkar N, El-Gindi O, Sayed S, Mohamed S, Ali Z, Alfishawy I. 2013. Antioxidant, anticancer and hepatoprotective activities of Cotoneaster horizontalis Decne extract as well as αtocopherol and amygdalin production from in vitro culture. Acta Physiol. Plant., 35: 2421-2428.

Thangapazham RL, Sharma A, Maheshwari RK. 2006. Multiple molecular targets in cancer chemoprevention by curcumin. The AAPS Journal. 8(3): 443.

Uysal A, Zengin G, Mollica A, Gunes E, Locatelli M, Yilmaz T, Aktumsek A. 2016. Chemical and biological insights on Cotoneaster integerrimus: a new (-)- epicatechin source for food and medicinal applications. Phytomedicine, 23: 979- 988.

Zengin G, Uysal A, Gunes E, Aktumsek A. 2014. Survey of phytochemical composition and biological effects of three extracts from a wild plant (Cotoneaster nummularia Fisch et Mey.): a potential source for functional food ingredients and drug formulations PLoS One, 9(11): e113527.