Oseni KADIRI, Gbadamosi Olasunkanmi SAKA, Babatunde OLAWOYE

Changes in TotalPhenolic and Protein Contents during Production of ProteinIsolates from Carica papaya(Pawpaw) Seeds

Oseni Kadiri*, Gbadamosi Olasunkanmi Saka, Babatunde Olawoye, Charles Taiwo Akanbi

The effect of different processing stages-defatting, solubilization, isoelectric precipitation, neutralisation and lyophilisation on the total phenolic content and protein content of Carica papaya (pawpaw) seeds (CPS) full-fat flour (CFF), defatted flour (CDF), protein concentrates (CPC) and protein isolates (CPI) wasstudied. The folin-ciocalteu procedure was used to assess the total phenolic concentrations of the Carica papaya products and results were expressed as gallic acid equivalent (GAE). Optimal protein content was estimated at pH 10 for all samples. Higherlevels of polyphenols were extracted using water-methanol (20/80) mixture compared to 100% methanol. Total phenolic content (TPC) was demonstrated in a trend of CDF > CPC > CFF > CPI with CDF having the highest TPC. The finding suggested a more economicalprotein isolate production method. The study concludes that the processing of CPS into the various products (CFF, CDF, CPC & CPI) improves its protein and polyphenol content.

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Alireza SM, Bhagya S. 2009. Effect of recovery method on a different property of the mustard protein. World Journal of Dairy & Food Sciences, 4,100-106.

Chavan U, Mckenzie D, Shahidi F. 2001. Functional properties of protein isolates from beach pea (Lathyrus maritimus L.). Food Chem 74: 177-187.

Cheftel JC, Cuq JL, Lorient D.1985. Food Chemistry: Amino acids, peptides and proteins (edited by Fennema, O. R.). New York, NY: Marcel Dekker. pp. 127-228.

Dwikat M, Dini L. Antioxidant effect of aqueous Carica papaya seed extract. 2nd Conference on Biotechnology Research and Applications. 26-27 September 2010, An-Najah National University, Nablus, Palestine.

Eltayeb AS, Abou-Arab AA, Ali OA & Abu-Salem FM. 2011. Chemical composition and functional properties of flour and protein isolate extracted from Bambara groundnut (Vigna subterranean). African Journal of Food Science, 5(2), 82 - 90

Farooq A, Hafiz MAQ, Abdullah IH, Shahid I. 2010. Antioxidant activity of 100% and 80% methanol extracts from barley seeds (Hordeum vulgare L.): stabilization of sunflower oil. Grasasy Aceities (6) 13 :237-243, 2010.

Gbadamosi SO, Abiose, SH, Aluko, RE. 2012. Amino acid profile, protein digestibility, thermal and functional properties of Conophor nut (Tetracarpidium conophorum) defatted flour, protein concentrate and isolates. Inter J. Food Sci Tech 47: 731- 739.

Gulcin I, Oktay M, Kirecci E, Kufrevioglu, OI. 2003. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food Chem 83: 371.

Iyawe HOT, Azih MC. 2011. Total phenolic contents and lipid peroxidation potentials of some tropical antimalarial plants. Eur. J. Med Plants 1(2): 33-39.

Jaime SAT, Rashid Z, Nhut DT, Sivakumar D, Gera A, Manoel TS, Tennant PF. 2007. Papaya (Carica papaya L.) Biology and Biotechnology. Tree and Forestry Science and Biotechnology 1(1):47-73.

Jayasena V, Chih, HJ & Dods K. 2006. Precipitation pH vs. yield and functional properties of lupin protein isolate. Agribusiness Crop Updates.

Kadiri O, Olawoye B. 2016. Vernonia amygdalina: An underutilized vegetable with nutraceutical Potentials - A Review. Turkish Journal of Agriculture - Food Science and Technology, 4(9): 763-768.

Kadiri O. 2015. Studies on the chemical composition, functional and antioxidant properties of Carica Papaya (Pawpaw) seed flour, protein concentrate and protein. M.sc thesis. Department of Food Science and Technology, Obafemi Awolowo University, Ile-Ife, Nigeria.

Kadiri O, Olawoye B. 2015. Underutilized indigenous vegetable (UIV) In Nigeria: A richsSource of nutrient and antioxidants- A Review. Annals. Food Science and Technology, 16(2): 236-247.

Kain JR, Che Z, Sonda ST & Abu-kpawoh JC. 2009. Study on the effects of enzymatic hydrolysis on the physical, functional and defatted heat pressed meal flour (Arachis hypogaea L.). Pakistan Journal of Nutrition, 8,818-825.

Kiosseoglou A.1999. Physical characterization of thermally induced networks of lupin protein isolates prepared by isoelectric precipitation and dialysis. International Journal of Food Science Technology 34: 253-263.

Liu Q, Yao H. 2007. Antioxidant activities of barley seeds extracts. Food Chem 102, 732-737.

Lqari H. 2002. Lupinus angustifolius protein isolates: chemical composition, functional properties and protein characterization. Food Chem. 76: 349-356.

Markwell MAC, Haas SM, Biebar LL, Tolbert, NE. 1978. A modification of the Lowry procedure to simplify protein determination in membranes and in protein samples. Analy Biochem 87: 206-211.

Oloyede O, Roos D, Rocha J. Studies on the Antioxidant Potential of Extracts from Unripe Fruit of Carica papaya. J. Life Sci 2012; 6:41-47.

Pierson JT, Dietzgen RG, Shaw PN, Roberts-Thomson SJ, Monteith GR, Gidley MJ.2012. Major Australian tropical fruits biodiversity: bioactive compounds and their bioactivities. Molec Nut & Food Res 56: 357-87

Quan V, Vuong, SH, Paul D, Roach MC, Bowyer, Phoebe A, Phillips, Christopher J. Scarlett . 2013. Effect of extraction conditions on total phenolic compounds and antioxidant activities of Carica papaya leaf aqueous extracts. J Herb Med 3; 104-111.

Ruiz LP, Hove EL .1976. Conditions affecting production of a protein isolate from lupin seed kernels. 27(7): 667-674.

Sanchez-Vioque R.1999. Protein isolates from chickpea (Cicer arietinum L.): chemical composition, functional properties and protein characterization. Food Chem. 64: 237-243.

Sathe, SK. 1994. Solubilization and electrophoretic characterization of cashew nut (Anacardium occidentale) proteins. Food Chem 51: 319-324.

Siddiq A, Anwar F, Manzoor M, Fatima M. 2005.Antioxidant activity of different solvent extracts of Moringa oleifera leaves under accelerated storage conditions of sunflower oil. Asian J. Plant Sci. 4, 630-635.

Singleton VL, Rossi JA. 1965. Colorimetry of total phenolic with phosphomolybdic phosphotungstic acid reagents. Am J. Enolo Viticult 16: 144-158.

Tsao R. 2010. Chemistry and biochemistry of dietary polyphenols. Nutr 2: 1231-46.

Visioli F, De La Lastra CA, Andres-Lacueva C, Aviram M, Calhau C, Cassano A. 2011.Polyphenols and human health: a prospectus. Crit Rev Food Sci Nut ;51:524-46.

Ulloa AJ, Rosas-Ulloa P & Physicochemical and functional properties of a protein isolate produced from safflower (Carthamus tinctorius L.) meal by ultrafiltration. Journal of Science of Food and Agriculture, 91, 572-577. Ulloa-Rangel BE. 2011.

Vuong QV. Epidemiological evidence linking tea consumption to human health: a review. 2012. Crit Rev Food Sci Nut. http://dx.doi.org/10.1080/ 2011.594184.

Wang H. J. and Murphy, PA. 1994. Isoflavone content in commercial soybean foods. Journal of Agricultural and Food Chemistry. 42 (8): 1666-1673.

Yu-Wei L, Wang Q. 2015. Effect of processing on phenolic content and antioxidant activity of four commonly consumed pulses in China. J Horticulture 2: 130. doi:10.4172/2376-0354.1000130

Zhou KB, Wang H, Mei WL, Li X, Luo Y, Dai HF. Antioxidant activity of papaya seed extracts. Mole 16 (8): 6179-6192.