Lukman Oladimeji RAJİ, Mohammed BABASHANİ, Ganiyu Jimoh AKOREDE, Aishat O OLATUNJİ, Fatima SANUSİ, Yusuf IDRIS, Khalid Tahlia BIOBAKU

Changes in Semen, Hormonal profile and Testicular Morphology of West African Dwarf Goat Bucks treated with Danazol

This study was carried out to investigate the changes in semen characteristics, hormonal profile and testicular morphometry of West African Dwarf (WAD) goat bucks treated with danazol. For this purpose, eighteen matured WAD bucks average of about two years were randomly divided into groups A, B and C comprising of six bucks per group. Group A was the control while B and C bucks were given danazol at a dose rate of 20mg/kg body weight orally daily for four weeks. The group B bucks’ testes were harvested thereafter for gross and histo-morphometric studies while those of group C were left intact but danazol treatment was withdrawn for four more weeks. The bucks’ semen samples (collected by electro-ejaculation) and hormonal samples (taken via the jugular vein) were analyzed. The semen characteristics studied included color, volume, mass activity, motility, percentage normal live-dead ratio, morphology and concentration; while the hormones studied included testosterone, follicle stimulating hormone and luteinizing hormone. Results show that there were significant decreases in semen characteristics of group B and C bucks compared with those of group A in the first four weeks. The semen characteristics of the group C bucks were reversed to normal range (similar to those of group A bucks) two weeks after danazol treatment was withdrawn. Similar results were observed with the hormonal studies. In conclusion, danazol caused a reversed reduction in sperm cells characteristics suggesting its possible use as a contraceptive in WAD goat bucks

PDF

___

Alvarez GC, Gómez-Galicia D, Rodríguez-Fragoso L, Marina VM, Dorantes LC, Sánchez-Aleman M, Mendez-Sanchez N, Esparza JR. 2011. Danazol improves thrombocytopenia in HCV patients treated with peginterferon and ribavirin. Annals of hepathology, 10 (4): 458-468.

Akinloye AK, Abatan MO, Alaka OO, Oke BO. 2002. Histomorphometric and Histopathological Studies on the effect of Calotropis procera (Giant milkweed) on the male reproductive organs of wistar rats. Afric. J. of Biomed. Res., 5: 57–61.

Ansari AS, Badar A, Lohiya NK. 2017. Fertility Control Modalities in Animals: An Overview. BAOJ Vet. Sci., 1: 004.

Bitto II, Akusu MO, Egbunike GN, Akpokodje JU. 2000. The physical characteristics of ovine-caprine ejaculate mixtures and the survival of their spermatozoa in cow’s milk extender in the humid tropics. Trop. J. of Anim. Sci., 3: 147-152.

Fesseha H. 2019. Non-surgical sterilization methods in male animals: A review. Vet. Med. Open J. 2019; 4(1): 49-56.

Jana K, Samanta PK, 2011. Clinical Evaluation of Non-surgical Sterilization of Male Cats with Single Intra-testicular Injection of Calcium Chloride. BMC Veterinary Research, 7:39-54.

Kollin C, Hesser U, Ritzen EM, Karpe B. 2006. Testicular growth from birth to two years of age and the effect of orchidopexy at age nine months: a randomized, controlled study. Acta Paediatrica, 95: 318–324.

Laoye BJ, Ishola AO, Mabayoje SO, Ogunlade BO, Olajuyigbe AB. 2019. Histoarchitecture of the Germinal Epithelium of Cyanide Treated Rats. EC Emergency Medicine and Critical Care 3(11): 14-20.

Lauro FV, Francisco DC, Ma LR, Elodia GC. 2011. Activity induced by a danazol derivative on perfusion pressure and coronary resistance in isolated rat heart. Biomedical Research, 22 (3): 289-294.

Lohiya NK, Sharma OP. 2009. Reversible inhibition of spermatogenesis by danazol with combination of testosterone enanthate in rabbit. Andrologia, 16(1): 72-5.

Mbaeri TU, Orakwe JC, Nwofor AM, Oranusi KC, Mbonu OO. 2012. Accuracy of Prader orchidometer in measuring testicular volume. Nig. J. Clin. Prac., 16: 348–351.

Oduwole OO, Peltoketo H, Huhtaniemi IT. 2018. Role of Follicle-Stimulating Hormone in Spermatogenesis. Front. Endocrinol., 9:763-773.

Olukole SG, Oyeyemi MO, Oke BO. 2014. Biometrical and histometrical observations on the testis and epididymis of the African sideneck turtle (Pelusio scastaneus). Eur. J. Anat., 18(2): 102-108.

Raji LO, Ajala OO. 2015. Scrotal Circumference as a Parameter of Breeding age for West African Dwarf Bucks. Turk. J. Agric. Food Sci. and Tech., 3(8): 668-671.

Raji LO, Ajala OO, Ameen SA. 2016. Testicular ultrasound as a breeding soundness examination and biometric tool for west african dwarf buck goats. Slovak J. Anim. Sci., 49(1): 8–16.

Raji LO, Ajala OO, Osuagwuh UI. 2015. Morphological and Morphometric Studies on the Epididymis of West African Dwarf Buck Goat (Capra hircus). J. Vet. Anat., 8(2): 69-80.

Van Kroonenburgh MJ, Beck JL, Vemer HM, Thomas CMG, Rolland R, Herman CJ. 1986. Effects of Danazol on spermatogenesis in adult rats. J. Reprod. Fertil., 77: 23-238.