ZEYNEP SÖNMEZ, MEMİŞ ÖZDEMİR, VECİHİ AKSAKAL

Büyüme Hormonu Geni Polimorfizminin Buzağı Doğum Ağırlığı Üzerine Etkisi

Bu çalışmada amaç, PCR-RFLP metodu kullanılarak elde edilen Sığır Büyüme Hormonu (bGH) geni genotipik yapıları ile 94 baş Holstein Buzağının doğum ağırlığı arasındaki ilişkileri incelemek, sürüye ait genotip ve allel frekansları dağılımını belirleyerek, genetik varyasyonu ortaya koymaktır. Çalışmada, buzağı doğum ağırlığı üzerine bGH geni LL genotipi 41,9 kg, LV 40,8 kg ve VV genotipi ise 42,4 kg olarak tespit edilmiştir. Tespit edilen genotipik yapılar ile buzağıların doğum ağırlığı özelliği arasındaki ilişkiler istatistiksel olarak anlamlı bulunamamıştır. Buzağı popülasyonunun bGH geni bakımından Hardy-Weinberg genetik denge testine göre dengede olmadığı görülmüştür.

Effect of Growth Hormone Genetic Polymorphism on Calf Birth Weight

The aim of study were to present relationships between birth weight of 94 Holstein calves with genotype structures of each sample’s Growth Hormone (GH) determined through PCR-RFLP method and to detect the population’s genetic variation by determining the genotype and allel frequency distribution. In the study, according to the analysis result, it was determined that Growth Hormone gene LL genotype was 41.9 kg, LL was 40.8 kg, and VV was 42.2 kg for the birth weight. A significant relationship between birth weight with GH genotypes could not be found. It was seen that the calf population is not stable according to Hardy-Weinberg genetic balance test.

PDF

___

NCBI. 2017 ‘’https://www.ncbi.nlm.nih.gov’’. The National Center for Biotechnology Information
Bass JJ, Oldham JM, Hodgkinson SC, Fowke PJ, Sauerwein H, Molan P, Breier BH, Gluckman PD. 1991. Influence of nutrition and bovine growth hormone (GH) on hepatic GH binding, insulin-like growth factor-I and growth of lambs. J. Endocrinol, 128(2):181-186.
Bauman DE. 1992. Bovine somatotropin: review of an emerging animal technology. J Dairy Sci. 75(12): 3432-51.
Cecim M, Kerr J, Bartke A. 1995. Effects of bovine growth hormone (bGH) transgene expression or bGH treatment on reproductive functions in female mice. Biology of Reproduction 52: 1144-1148.
Dybus A. 2002. Associations between Leu/Val polymorphism of growth hormone gene and milk production traits in Blackand-White cattle. Arch. Tierz. Dummerstorf 45(5): 421-428.
Dybus A, Kmiec M, Sobek Z, Wisniewski B. 2003. Associations between polymorphism of the growth hormone gene and production traits of Limousine cattle. Medycyna Weterynaryjna, 59(2): 133-136, Abstr.
Echeverri J, Saldamando CI, Herrera AL. 2015. Genetic structure analysis of a Holstein cow population in Colombia. Rev Colomb Cienc Pecu; 28: 54-63.
Falaki M, Gengler N, Sneyers M, Prandi A, Massart S, Formigoni A, Burny, A, Portetelle D, Renaville R., 1996. Relationships of polymorphisms for growth hormone and growth hormone receptor genes with milk production traits for Italian Holstein-Friesian bulls. J. Dairy Sci.79(8):1446- 1453.
Fedota OM, Ruban S, Yu Lysenko NG, Kolisnyk AI, Goraichuk IV, Tyzhnenko TV.,2016. SNP L127V of Growth Hormone Gene In Breeding Herd of Aberdeen Angus In Kharkiv Region, Eastern Ukraine. Journal for Veterinary Medicine, Biotechnology and Biosafety 2(3): uDC 575.113.1:636.223.1(477.54).
Fontanesi L, Scotti E, Samore AB, Bagnato A, Russo V. 2015. Association of 20 candidate gene markers with milk production and composition traits in sires of Reggiana breed, a local dairy cattle population. Livestock Science, 176: 14-21.
Fries R, Egen A, Womack JE. 1993. The bovine genome map. Mammalian Genome. 4:405.
Ge W, Davis ME, Hines HC, Irvin KM, Simmen CM. 2003. Association of single nucleotide polymorphisms in the growth hormone and growth hormone receptor genes with serum insulin-like growth factor I concentration and growth traits in Angus cattle. J. Anim. Sci., 81: 641–648.
Grochowska R, Lunden A, Zwierzchowski L, Snochowski M, Oprzadek J. 2001. Association between gene polymorphism of growth hormone and carcass traits in dairy bulls. Anim. Sci. 72: 441-447.
Höglund JK, Sahana G, Brondum RF, Guldbrandtsen B, Buitenhuis B, Lund MS. 2014. Fine mapping QTL for female fertility on BTA04 and BTA13 in dairy cattle using HD-SNP and sequence data. BMC Genomics, 15:790. http://www.biomedcentral.com/1471-2164/15/790.
Ishida T, Umebayashi A, Tsuruta S, Akashi R, Harada H. 2010. Polymorphisms in growth hormone gene and their associations with calf weight in Japanese Black cattle. Animal Science Journal, 81: 623–629.
Klein RJ, Zeiss C, Chew EY, Tsai JY, Sackler RS, Haynes C. 2005. Complement Factor H Polymorphism in Age-Related Macular Degeneration. Science, 308 (5720): 385-389. Doi:10.1126/science.1109557.
Kovacs K, Volgyi-Csik J, Zsolnai A, Gyorkos I, Fesus L. 2006. Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population. Archiv fur Tierzucht, 49: 236–249.
Lagziel A, Lipkin E, Ezra E, Soller M, Weller JI. 1999. An MspI polymorphism at the bovine growth hormone (bGH) gene is linked to a locus affecting milk protein percentage. Animal Genetics, 30: 296-299.
Mattos KK, Del-Lama SN, Martinez ML, Freitas F. 2004. Association of bGH and Pit-1 gene variants with milk production traits in dairy Gyr bulls. Pesq. Agropec. Bras, 39(2): 147-150.
Özdemir M. 2011. Determination of allele frequencies of growth hormone AluI polymorphism in Brown Swiss, Holstein, native East Anatolian Red and Turkish Grey breeds. International Journal of Dairy Technology. 64(3):350–352.
Özdemir M, Sönmez Z, Topal M. 2015. Organik yetiştirilen Holstein Irkında bGH/Alu I ve CSN3/Hinf I Gen Polimorfizmlerinin Belirlenmesi. 9. Ulusal Zootekni Bilim Kongresi, P838-847, 3-5 Eylül 2015, Konya.
Pal A, Chakravarty AK, Bhattacharya TK, Sharma A. 2005. Polymorphism of growth hormone gene and its association with expected milk production traits in dairy bulls. J. Appl. Anim. Res. 27(1): 29-33.
Paputungan U, Hakim L, Ciptadi G, Lapian HFN. 2015. Herıtabılıtıes Of Body Sıze By Growth Hormone (GHMsp1) Genotypes Usıng PCR-RFLP In Ongole Grade Cattle. J. Indonesian Trop. Anim. Agric. 40(3):138-144.
Sabour MP, Lin CY, Smith C. 1997. Association of genetic variants of bovine growth hormone with milk production traits in Holstein cattle. Journal of Animal Breeding and Genetics, 114: 435-442.
Schlee P, Graml R, Schallenberger E, Schams D, Rottmann O, Olbrich BA, Pirchner F. 1994. Growth hormone and insulin like growth factor I: concentration in bulls of various growth hormone genotypes. Theoretical and Applied Genetics, 88: 497-500.
Taylor JF, Coutinho LL, Herring KL, Gallagher JDS, Brenneman RA, Burney N, Sanders JO, Turner JW, Smith SB, Miller RK, Savell JW, Davis SK. 1998. Candidate gene analysis of GH1 for effects on growth and carcass composition of cattle. Anim. Genet. 29: 194-201.
Unanian MM, Barreto CC, Freitas AR, Cordeiro CMT, Josahkian LA. 2000. Association of growth hormone gene polymorphisms with weight traits in Nelore breed.. Rev. Bras. Zootec. 29:1380-1386.
Wang X. 2012. Identification and Characterization of Candidate Genes for Complex Traits in Cattle. PhD thesis, Chair of Animal Breeding, Technische Universität München.
Yao J, Aggrey SE, Zadworny D, Hayes JF, Kuhnlein U. 1996. Sequence variations in the bovine growth hormone gene characterized by single-strand conformation polymorphins (SSCP) analysis and their association with milk production traits in Holsteins. Genetics. 144: 1809-1816.
Zhang HM, Brown DR, Denise SK, Ax RL. 1992. Nucleotide sequence determination of a bovine somatotropin allele. Animal Genetics 23 (6): 578.
Zho G., Zhu Q, Jin HG, Guo SL. 2006. Genetic variation of growth hormone gene and its relationship with milk production traits in China Holstein cows. Asian-Australasian J. of Anim. Sci. 19(3): 315-318.