Aytül UÇAK KOÇ, Mete KARACAOĞLU, Zehra Burcu BAKIR, Burcu KESER

Arı Sütü Üretim Kolonilerinin Anasız ve Ana Arılı Olması Arı Sütündeki 10- HDA ve Çözünür Protein Miktarını Etkiler mi?

Arı sütü, diyetlerin düzenlenmesinde ve kozmetik endüstrisinde de fonksiyonel gıda maddesi olan önemli bal arısı ürünlerinden biridir. Arı sütü, insanlarda çeşitli hastalıkları tedavi edici potansiyele sahiptir ve kimyasal içeriği bazı faktörlerden etkilenir. Bu çalışmada arı sütü üretim kolonilerinde ana arı varlığının ve yokluğunun arı sütündeki 10-hidroksi-2-dekenoik asit (10-HDA) ve çözünür protein miktarı üzerine etkisi belirlenmiştir. Bu nedenle koloniler anasız, ana arılı ve başlatıcı-bitirici olarak hazırlanmıştır. Arı sütü verimleri anasız, ana arılı ve başlangıç-bitirici kolonilerinde sırasıyla; 15,2 ± 0,89 g, 12,0 ± 0,90 g ve 9,6 ± 0,72 g olarak belirlenmiştir. Anasız grup diğer iki gruptan farklı bulunmuştur. Anasız (%2,0 ± 0,06) ana arılı (%2,1 ± 0,06) ve başlatıcı-bitirici (%2,0 ± 0,05) kolonilerin 10-HDA değerleri benzer iken; çözünür protein miktarları ise, anasız (%9,65 ± 0,179), ana arılı (%7,68 ± 0,184) ve başlatıcı-bitirici (%7,50 ± 0,203) koloniler birbirinden farklı ve önemlidir. Sonuç olarak, arı sütü üretim kolonisinin anasız veya ana arılı olarak hazırlanması arı sütü çözünür protein miktarını etkilemiştir.

Does the Presence and Absence of Queen Bee in the Production of Royal Jelly Affect the Amount of Soluble Protein and Ratio of 10-Hydroxy-2-Decenoic Acid?

Royal jelly (RJ), is one of the important honey bee products and a functional food item in theregulation of diets and in the cosmetic industry. RJ has a potential towards various human diseasetreatments. The chemical content of RJ is influenced by some factors. In this study, the effect of thepresence or absence of the queen on the amount of 10-hydroxy-2-decenoic acid (10-HDA) and solubleprotein in RJ is determined. For this reason, colonies were prepared as queenless, queenright andstarter-finisher. RJ yields in colonies queenless, queenright and starter-finisher were determined as15.2 ± 0.89 g, 12.0 ± 0.90 g and 9.6 ± 0.72 g, respectively. Group queenless was different from theother two groups. While 10-HDA values of the groups were similar (queenless, queenright andstarter-finisher, respectively; 2.0 ± 0.06%, 2.1 ± 0.06% and 2.0 ± 0.05%), the soluble protein amountsof the groups (queenless, queenright and starter-finisher, respectively, 9.65 ± 0.179%, 7.68 ± 0.184%,7.50 ± 0.203%) were found different and significant from each other. As a result, the RJ productioncolony queenless or queenright affected the amount of soluble protein. The worker bees of queenlesscolonies secreted RJ containing more soluble protein.

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Araujo WLP, Negrao AF, Vieira JC, S Bittarello AC, Padilha PM, Orsi RO. 2020. Biological Trace Element Research, 195:648– 657.
Arzi A, Olapour S, Yaghooti H, Karampour NS. 2015. Effect of royal jelly on formalin induced-inflammation in rat Hind Paw. Jundishapur J Nat Pharm Prod. February; 10(1): e22466.
Balkanska R. 2018. Determination of Trans-10-Hydroxy-2- Decenoic Acid in Royal Jelly by High Performance Liquid Chromatography after Different Bee Feeding. International Journal of Current Microbiology and Applied Sciences, 7(4): 3738-3743.
Barnutiu LI, Dezmirean DS, Mihai CM, Bobis O. 2011. Chemical composition, and antimicrobial activity of royal jelly–review. Journal of Animal Science and Biotechnologies, 44 (2): 67-72.
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.
Caparica C, Marcucci S, Marcucci MC. 2007. Quantitative determination of trans- 10-Hydroxy-2-Decenoic Acid (10- HDA) in Brazilian royal jelly and commercial products containing royal jelly. Journal of Apicultural Research and Bee World, 46(3): 149–153.
Cengiz M, Emsen B, Dodologlu A. 2009. Some characteristics of queenbees (Apis mellifera L.) rearing in queenright and queenless colonies. Journal of Animal and Veterinary Advances, 8 (6): 1083-1085.
Chen D, Liu F, Wan JB, Lai CQ, Shen LR. 2017. Effect of major royal jelly proteins on spatial memory in aged rats: Metabolomics analysis in urine. Journal of Agricultural and Food Chemistry, 65(15): 3151–3159.
El-Nekeety AA, El-Kholy W, Abbas NF, Ebaid A, Amra HA, Mosaad AV. 2007. Efficacy of royal jelly against the oxidative stress of fumonisin in rats. Toxicon, 50 (2):256– 269. doi: 10.1016/j.toxicon.2007.03.017.
Evans JD, Wheeler DE. 1999. Differential gene expression between developing queens and workers in the honeybee, Apis mellifera. Proceedings of the National Academy of Sciences of the United States of America. 96 (10): 5575– 5580.
Evans JD, Wheeler DE. 2001. Expression profiles during honeybee caste determination. Genome Biology, 2 (1): 1-6.
Fan P, Han B, Feng M, Fang Y, Zhang L, Hu H, Hao Y, Qi Y, Zhang X, Li J. 2016. Functional and proteomic investigations reveal major royal jelly protein 1 associated with antihypertension activity in mouse vascular smooth muscle cells. Scientific Reports | 6:30230 | DOI: 10.1038/srep30230.
Fratini F, Cilia G, Mancini S, Felicioli, A. 2016. Royal Jelly: An ancient remedy with remarkable antibacterial properties. Microbiological Research, 192, 130–141.
Hu FL, Bilikova K, Casabianca H, Daniele G, Espindola SF. et al. 2017. Standard methods for Apis mellifera royal jelly research. Journal of Apicultural Research, 58: 1–69.
Isidorov VA, Bakier S, Grzech I. 2012. Gas chromatographicmass spectrometric investigation of volatile and extractable compounds of crude royal jelly. Journal of Chromatography B, (885–886): 109–116.
Kamakura M, Fukuda T, Fukushima M, Yonekura M. 2001. Storage-dependent degradation of 57-kDa protein in royal jelly: A possible marker for freshness. Bioscience, Biotechnology, and Biochemistry, 65, 277–284.
Kamyab S, Gharachorloo M, Honarvar M, Ghavami M. 2020. Quantitative analysis of bioactive compounds present in Iranian royal jelly. Journal of Apicultural Research, 59(1), 42–52.
Kanbur M, Eraslan G, Beyaz L, Silici S, Liman, BC, Altınordulu Ş, Atasever A. 2009. The effects of royal jelly on liver damage induced by paracetamol in mice. Experimental and Toxicologic Pathology, 61(2):123–132.
Kanelis D. Tananaki C, Liolios V, Dimou M, Goras G, Rodopoulou MA, Karazafiris E, Thrasyvoulou, A. 2015. A suggestion for royal jelly specifications. Arh Hig Rada Toksikol, 66:275-284.
Karacaoğlu M, Kösoğlu M, Uçak Koç A. 2004. Farklı yöntemlerin Ege ekotipi (A. m. anatolica) ve Kafkas (A. m. caucasica) x Ege melezi bal arılarının arı sütü verimleri üzerine etkileri. ADÜ Ziraat Fakültesi Dergisi, 1(1): 29-33.
Karacaoğlu M, Uçak Koç A, Bakır BZ, Metin K, Keser B, Birincioglu B. 2019. The effect of harvest time and number of queen cell on 10-HDA and total protein content in royal jelly. 11. International Animal Science Conference, Cappadocia, Turkey, 20-22 October, p:380-384.
Kim J, Lee J. 2010. Quantitative analysis of trans-10hydroxy-2- decenoic acid in royal jelly products purchased in USA by high-performance liquid chromatography. Journal of Apicultural Science, 54(1), 77–85.
Kocot J, Kiełczykowska M, Luchowska-Kocot D, Kurzepa J, Musik I. 2018. Antioxidant potential of propolis, bee pollen, and royal jelly: possible medical application. Hindawi Oxidative Medicine and Cellular Longevity, Article ID 7074209, 29 pages https://doi.org/10.1155/2018/7074209.
Kohno K, Okamoto I, Sano O. 2004. Royal jelly inhibits the production of proinflammatory cytokines by activated macrophages. Bioscience, Biotechnollogy and Biochemistry, 68:138–145.
Kösoğlu M, Yücel B, Gökbulut C, Konak R, Bı̇rcan C. 2013. The effect of harvesting time on some biochemical and trace element compositions of royal jelly. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(2), 233-237.
Liu JR, Yang YC, Shi LS, Peng CC. 2008. Antioxidant Properties of Royal Jelly Associated with Larval Age and Time of Harvest. Journal of Agricultural Food Chemistry, 56, 11447– 11452.
Martinez-Dominguez G, Romero-Gonzalez R, Garrido Frenich A. 2016. Multi-class methodology to determine pesticides and mycotoxins in green tea and royal jelly supplements by liquid chromatography coupled to Orbitrap high resolution mass spectrometry. Food Chemistry, 197: 907-915.
Matsushita H, Shimizu S, Morita N, Watanabe K, Wakatsuki A. 2020. Effects of royal jelly on bone metabolism in postmenopausal women: a randomized, controlled study. Climacteric https://doi.org/10.1080/13697137.2020.1806815
Morita H, Ikeda T, Kajita K.et al. 2012. Effect of royal jelly ingestion for six months on healthy volunteers. Nutrition Journal, 11(77) :1-7.
Moritz RF, Lattorff HM, Neumann P, Kraus FB, Radloff SE, Hepburn HR. 2005. Rare royal families in honeybees, Apis mellifera. Naturwissenschaften, 92 (10): 488–491.
Nakajima Y, Tsuruma K, Shimazawa M, Mishima S, Hara H. 2009. Comparison of bee products based on assays of antioxidant capacities. BMC Complementary and Alternative Medicine, 9(4):1-9.
Nozaki R, Tamura S, Ito A, Moriyama T, Yamaguchi K, Kono T. 2012. A rapid method to isolate soluble royal jelly proteins. Food Chemistry, 134, 2332–2337.
Okamoto I, Taniguchi Y, Kunikata T, Kohno K, Iwaki K, Ikeda M, Kurimoto M. 2003. Major royal jelly protein 3 modulates immune responses in vitro and in vivo. Life Sciences, 73, 2029–2045.
Ramadana MF, Al-Ghamdi A. 2012. Bioactive compounds and health-promoting properties of royal jelly: A review. Journal of Functional Foods, 4: 39 –52.
Sabatini AG, Marcazzan GL, Caboni MF, Bogdanov S, De Almeida-Muradian LB. 2009. Quality, and standardisation of royal jelly. Journal of ApiProduct and ApiMedical Science, 1(1): 1–6.
Sahinler N, Kaftanoglu O. 2005. The effects of season and honeybee (Apis mellifera L.) genotype on acceptance rates and royal jelly production. Turkish Journal of Veterinary and Animal Sciences, 29:499-503.
SAS. 1999. Statistical Analsis System for Windows (Relase 8.2). SAS Institute Inc.Raleigh, Caroline, USA.
Scarselli R, Donadio E, Giuffrida MG, Fortunato D, Conti A, Balestreri E, Felicioli R, Pinzauti M, Sabatini AG, Felicioli A. 2005. Towards royal jelly proteome. Proteomics, 5, 769– 776.
Seyyedi F, Rafiean-Kopaei M, Miraj S. 2016. Comparison of the effects of vaginal royal jelly and vaginal estrogen on quality of life, sexual and urinary function in postmenopausal women. Journal of Clinical and Diagnostic Research, 10(5): QC01-QC05
Simuth J. 2001. Some properties of the main protein of honey bee (Apis mellifera) royal jelly. Apidologie, 32, 69–80.
Stocker A, Schramel P, Kettrup A, Bengsch E. 2005. Trace and mineral elements in royal jelly and homeostatic effects. Journal of Trace Elements in Medicine and Biology, 19 (23): 183–189.
Xin XX, Chen Y, Chen D, Xiao F, Parnell LD, Zhao J. Shen LR. 2016. Supplementation with major royal-jelly proteins increases lifespan, feeding, and fecundity in drosophila. Journal of Agricultural and Food Chemistry, 64(29), 5803- 5812.
Wang X, Cook LF, Grasso LM, Cao M, Dong Y. 2015. Royal jelly-mediated prolongevity and stress resistance in caenorhabditis elegans is possibly modulated by the interplays of DAF-16, SIR-2.1, HCF-1, and 14-3-3 proteins. Journals Gerontology Series A: Biological Sciences and Medical Sciences, 70 (7): 827-838.
Wytrychowski M, Chenavas S, Daniele G, Casabianca H, Batteau M, Guibert S, Brion B. 2013. Physicochemical characterisation of French royal jelly: Comparison with commercial royal jellies and royal jellies produced through artificial bee-feeding. Journal of Food Composition and Analysis, 29 (2):126–133.
Zheng HQ, Hu FL, Dietemann, V. 2011. Changes in composition of royal jelly harvested at different times: consequences for quality standards. Apidologie, 42:39–47.