Antioxidant Activity of Mytilus galloprovincialis and Ruditapes philippinarum

This study reports on the evaluation of the antioxidant of ethanolic extracts of Mytilus galloprovincialis and Ruditapes philippinarum collected from the Marmara Sea. The antioxidant activity of the two bivalve species was evaluated using the 2,2-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. The total carotenoid contents were also determined. In the DPPH method, all extracts exhibited low radical scavenging activity when compared to the standards used, i.e. -tocopherol and butylated hydroxytoluene (BHT) with the inhibition percentage in the range of 35.87±0.05 – 36.27±0.02%. In particular, M. galloprovincialis exhibited the highest radical scavenging activity with an IC50 value of 29.55±0.02 mg/g Ext. However, there are no statistically significant differences in the IC50 values of two species. The highest total carotenoid content was found in M. galloprovincialis as 1.13±0.02 μg/g sample. The statistically significant differences were found in total carotenoid contents of two bivalves. In this study in M. galloprovincialis was also found to be high in carotenoid content, while R. philippinarum was found to be almost nonexistent. More detailed studies are needed to determine relationship between the bioactive compounds of crustaceans and their antioxidant activities, distributed in our country.

___

Anbazahan SM, Mari LS, Yogeshwari G, Jagruthi C, Thirumurugan R, Arockiaraj J. 2014. Immune response and disease resistance of carotenoids supplementation diet in Cyprinus carpio against Aeromonas hydrophila. Fish Shellfish Immunol. 40, 9–13. DOI: 10.1016/j.fsi.2014.06.011

Albayrak S. 2005. First record of Tapes philippinarum (Adams and Reeve, 1850) (Bivalvia: Veneridae) from the Sea of Marmara. Zool Middle East 35(1):108–109. DOI: 10.1080/09397140.2005.10638113

Alasalvar C, Taylor Y. 2002. Seafoods –Quality Technology and Nutraceutical Applications. Springer-Verlag, Heidelberg, Germany, 508 pp:17-31. ISBN 978-3-662-09836-3

Bendich A, Olson JA. 1989. Biological actions of carotenoids. FASEB J. 3(8): 1927–1932. PMID: 2656356

Biehler E, Maye F, Hoffmann L, Krause E, Bohn T. 2010. Comparison of 3 Spectrophotometric Methods for Carotenoid Determination in Frequently Consumed Fruits and Vegetables. Journal of Food Science.75(1):C55-61. DOI: 10.1111/j.1750-3841.2009.01417.x.

Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci.Technol. 28(1): 25 – 30. DOI: 10.1016/S0023- 6438(95)80008-5

Caramujo MJ, de Carvalho CC, Silva SJ, Carman KR. 2012. Dietary carotenoids regulate astaxanthin content of copepods and modulate their susceptibility to UV light and copper toxicity. Mar. Drugs 10(5):998–1018. DOI: 10.3390/md10050998

Cui K, Luo X, Murthy MRV. 2004. Role of oxidative stress in neurodegeneration: recent developments in assay methods for oxidative stress and nutraceutical antioxidants. Prog Neuropsychopharmacol Biol Psych. 28(5):771–799. DOI: 10.1016/j.pnpbp.2004.05.023

Çolakoğlu S, Palaz M. 2014. Some population parameters of Ruditapes philippinarum (Bivalvia, Veneridae) on the southern coast of the Marmara Sea, Turkey. Helgol Mar Res. 68(4): 539 – 548. DOI: /10.1007/s10152-014-0410-7

Dridi S, Romdhane MS, Elcafsi M. 2007. Seasonal variation in weight and biochemical composition of the Pacific oyster, Crassostrea gigas in relation to the gametogenic cycle and environmental conditions of the Bizert Lagoon. Tunisia. Aquaculture. 263(1-4):238–248. DOI: 10.1016/j.aquaculture. 2006.10.028

FAO. 2009. Food and Agriculture Organization. State of the world fisheries and aquaculture, 2008. Rome: Fisheries and Aquaculture Department.

FAO. 2019. Fisheries and Aquaculture topics. Fisheries statistics and information. Topics Fact Sheets. In: FAO Fisheries and Aquaculture Department [online]. Rome. Updated 22 December 2015. [Cited 30 March 2019]. http://www.fao.org/fishery/

Fiedor J, Sulikowska A, Orzechowska A, Fiedor L, Burda K. 2012. Antioxidant effects of carotenoids in a model pigmentprotein complex. Acta Biochim. Pol. 59(1):61–64. PMID: 22428149

Halliwell B. 1996. Antioxidants in human health and disease. Annu Rev Nutr 16: 33– 50. DOI: 10.1146/annurev.nu. 16.070196.000341

Hill GE, Johnson JD. 2012. The vitamin A–redox hypothesis: a biochemical basis for honest signaling via carotenoid pigmentation. Amer. Nat. 180(5): E127–E150. DOI: 10.1086/667861

Kantha SS. 1989. Carotenoids of Edible Molluscs; A Review. J. Food Biochemistry, 13, 429-442.DOI: 10.1111/j.1745- 4514.1989.tb00410.x

Liaaen-Jensen S. 1998. Carotenoids in Food Chain, in Carotenoids: Biosynthesis and Metabolism, eds Britton G, Liaaen-Jensen S, Pfander H. (Basel: Birkhäuser), 359–371. ISBN: 9783764358297

Leiva GE, Castilla JC. 2001. A review of the world marine gastropod fishery: Evolution of catches, management and the Chilean experience. Reviews in Fish Biology and Fisheries 11(4):283-300. DOI: 10.1023/A:1021368216294

Lorenz T. 2010. A Review of the Carotenoid, Astaxanthin, as a Pigment Source and Vitamin for Cultured Penaeus Prawn, NatuRose Technical Bulletin 51. Pp: 1- 7. Available at: https://pdfs.semanticscholar.org/2322/b4bed5905f61477847 62713f82aaf254e7e3.pdf

Maoka T, Akimoto N. 2008. Carotenoids and their fatty acid esters of spiny lobster Panulirus japonicus. J. Oleo. Sci. 57(3): 145–152. PMID: 18270463

Maoka T. 2011. Carotenoids in marine animals. Mar. Drugs 9(2): 278–293. DOI: 10.3390/md9020278

Merdzhanova A, Dobreva DA, Panayotova V. 2018. Assessment of proximate and bioactive lipid composition of Black Sea Mussels (M. galloprovincialis) from Bulgaria. In: Biological Resources of Water. Ed: Ray S. Intech Open. London. ISBN: 978-1-78923-080-2, pp:181-200. DOI: 10.5772 /intechopen.71909

Moschino V, Delaney E, Da Ros L. 2012. Assessing the significance of Ruditapes philippinarum as a sentinel for sediment pollution: Bioaccumulation and biomarker responses. Environmental Pollution 171: 52 – 60. DOI:10.1016/j.envpol.2012.07.024

Mathiesen SS, Thyrring J, Hemmer-Hansen J, Berge J, Sukhotin A, Leopold P, Bekaert M, Sejr MK, Nielsen EE. 2017. Genetic diversity and connectivity within Mytilus spp. in the subarctic and Arctic. Evolutionary Applications. 10:39-55. DOI: 10.1111/eva.12415

Matsuno, T., 1989. Animal Carotenoids, in Carotenoids: Chemistry and Biology, Eds: Krinsky NI, Mathews-Roth MM, Taylor RF.Springer. Boston pp:59–74. ISBN: 978-1- 4613-0849-2

Mooney BD, Nichols PD, Elliot NG. 2002. Seafood the Good Food II: Oil profiles for further Australian seafood and influencing factors. Ed: Hobart T: CSIRO Division of Marine Research and Deakin, A.C.T.: Fisheries Research & Development Corporation (FRDC), Australia. ISBN: 1876996064

Jensen AC, Humphreys J, Caldow RWG, Grisley C, Dyrynda PEJ. 2004. Naturalization of the Manila clam (Ruditapes philippinarum), an alien species, and establishment of a clam fishery within Poole Harbour, Dorset. J Mar Biol Assoc UK 84(5):1069–1073. DOI:10.1017/S0025315404010446h

Orban E, Di Lena G, Nevigato T, Casini I, Marzetti A, Caproni R., 2002. Seasonal changes in meat content, condition index and chemical composition of mussels (Mytilus galloprovincialis) cultured in two different Italian sites. Food Chemistry, 77(1): 57-65. DOI: 10.1016/S0308- 8146(01)00322-3

Perera CO, Yen GM. 2007. Functional Properties of Carotenoids in Human Health, International Journal of Food Properties, 10:2, 201-230, DOI: 10.1080/10942910601045271

Shahidi F, Naczk M. 2004. Phenolics in Food and Nutraceuticals. CRC Press LLC, Florida. ISBN: 9781587161384 pp: 25-49.

Singh S, Singh RP. 2008. In vitro methods of assay of antioxidants: An overview. Food Rev Int. 24(4):392–415. DOI: 10.1080/87559120802304269

Tanaka Y, Katayama T. 1979. Carotenoids in Sea Mussel; Mytilus edulis. Mem. Fac. Fish., Kagoshima Univ. Vol. 28 : 9-16.

TUIK, 2010. Türkiye İstatistik Kurumu. Türkiye İstatistik Yıllığı, 2009 Ankara, Yayın No:3436.

Van Nieuwerburgh L, Wänstrand I, Liu J, Snoeijs P. 2005. Astaxanthin production in marine pelagic copepods grazing on two different phytoplankton diets. J. Sea Res. 53(3): 147– 160. DOI: 10.1016/j.seares.2004.07.003

Von Schantz T, Bensch S, Grahn M, Hasselquist D, Wittzell H. 1999. Good genes, oxidative stress, and condition-dependent sexual signals. Proc. R. Soc. Lond. B. Biol. Sci. 266, 1–12.

Voutilainen S, Nurmi T, Mursu J, Rissanen TH. 2006. Carotenoids and cardiovascular health. Am. J. Clin. Nutr. 83(6):1265–1271. DOI: 10.1093/ajcn/83.6.1265
Türk Tarım - Gıda Bilim ve Teknoloji dergisi-Cover
  • ISSN: 2148-127X
  • Yayın Aralığı: Aylık
  • Başlangıç: 2013
  • Yayıncı: Turkish Science and Technology Publishing (TURSTEP)