Pleurotus pulmonarius was cultivated in three different environmental conditions, in ambient indoor environment (System 1), in humidifying without ventilation (System 2) and in humidifying with ventilation (System 3) to analyse the major nutritional contents. Sawdust was the main substrate for all the cultivation systems. The lowest temperature and the highest optimal humidity were found in System 3. The temperature and humidity had shown statistically significant among the three cultivation Systems. The highest numbers of flushes was found both in System 2 and System 3 but System 1 was produced mushrooms till 3rd flush. About 29.5%, 28.3%, 28.5% protein; 59.0%, 55.8%, 54.3% carbohydrate and 3.8%, 3.5%, 3.3% lipid were found in System 1, System 2 and System 3 respectively. The protein, carbohydrate, and lipid contents were shown statistically insignificant among the cultivation systems. The highest value of protein, carbohydrate and lipid were found for the sample of 1st flush in all the cultivation systems but the values were started to decrease with the increased numbers of flushes significantly. So, this study shown that, although the environmental conditions of the three cultivation systems were varied significantly but the protein, carbohydrate and lipid contents were existed their normal values in all cases but the values were decreased by the increased numbers of flushes.
___
Abdul A, Rozainee M, Mutahharah M. 2011. Microwave sterilization of mushroom production media. Waste Management and Bioresource Technology, 1(1): 1–11.
Akindahunsi AA, Oyetayo FL. 2006. Nutrient and antinutrient distribution of edible mushroom, Pleurotus tuber-regium (fries) singer. Food Science and Technology, 39(5): 548–553.
Alam N, Amin R, Khan A, Ara I, Shim MJ, Lee MW, Lee TS. 2008. Nutritional analysis of cultivated mushrooms in Bangladesh - Pleurotus ostreatus, Pleurotus sajor-caju, Pleurotus florida and Calocybe indica. Mycobiology, 36(4): 228–32.
Ashraf J, Ali MA, Ahmad W, Ayyub CM, Shafi J. 2013. Effect of different substrate supplements on oyster mushroom (Pleurotus spp.) production. Food Science and Technology, 1(3): 44–51.
Caglarırmak N. 2007. The nutrients of exotic mushrooms (Lentinula edodes and Pleurotus species) and an estimated approach to the volatile compounds. Food chemistry, 105(3): 1188–1194.
Chang ST, Lau OW, Cho KY. 1981. The cultivation and nutritional value of Pleurotus sajor-caju. European Journal of Applied Microbiology and Biotechnology, 12(1): 58–62.
Chirinang P, Intarapichet KO. 2009. Amino acids and antioxidant properties of the oyster mushrooms, Pleurotus ostreatus and Pleurotus sajor-caju. Science Asia, 35: 326–331.
Christopher J. Custodio D. 2004. Substrate: Oyster mushroom cultivation. In Mushroom Grower’s Handbook 1. China: MushWorld, pp. 91–94. DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956.
Colorimetric method for determination of sugars and related substances. Analitical Chemistry, 28(3): 350–356.
Folch J, Lees M, Stanley GHS. 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226(1): 497–509.
Gogavekar SS, Rokade SA, Ranveer RC, Ghosh JS, Kalyani DC, Sahoo AK. 2014. Important nutritional constituents, flavour components, antioxidant and antibacterial properties of Pleurotus sajor-caju. Journal of Food Science and Technology, 51(8): 1483–1491.
Islam MT, Zakaria Z, Hamidin N, Ishak MABM. 2016a. Characteristics of indoor mushroom cultivation of grey oyster (Pleurotus pulmonarius) by different stages of humidifying treatment. World Applied Sciences Journal, 34(8): 1066–1075.
Islam MT, Zakaria Z, Hamidin N, Mohd Azlan BMI. 2016b. A competitive study on higher yield performance in indoor optimized environment and outdoor cultivation of Pleurotus pulmonarius. MAYFEB Journal of Agricultural Science 2: 13– 27.
Islam T, Zakaria Z, Hamidin N, Mohd Azlan BMI. 2016c. Optimization of humidifying procedure in controlled environment for indoor cultivation of Pleurotus pulmonarius. African Journal of Biotechnology. 15(45): 2578–2586.
Kadlag GK, Wani PV, Sawant DM. 1998. Comparative performance of different Pleurotus spp. on wheat and green gram straw. Maharashtra Agricultural University, 23(1): 25–86.
Khan MA, Amin SMR, Uddin MN, Tania M, Alam N. 2008. Comparative study of the nutritional composition of oyster mushrooms cultivated in Bangladesh. Bangladesh Journal of Mushroom, 2(1): 9–14.
Khatun S, Islam A, Cakilcioglu U, Guler P, Chatterjee NC. 2015. Nutritional qualities and antioxidant activity of three edible oyster mushrooms (Pleurotus spp.). NJAS - Wageningen Journal of Life Sciences, 72–73: 1–5.
Leiva FJ, Saenz-Diez JC, Martínez E, Jimenez E, Blanco J. 2015. Environmental impact of Agaricus bisporus cultivation process. European Journal of Agronomy, 71: 141–148.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193(1): 265–275.
Ma Y, Guan CY, Meng XJ. 2014. Biological characteristics for mycelial growth of Agaricus bisporus. In Applied Mechanics and Materials. Trans Tech Publ, pp. 297–302.
Maria EV, Talia HP, Octavio PL. 2015. Edible mushrooms: Improving human health and promoting quality life. International Journal of Microbiology, 2015: 1-15.
Miles PG, Chang ST. 2004. Mushrooms: cultivation, nutritional value, medicinal effect, and environmental impact. CRC Press, Bora Raton, London, New York, Washington DC, pp: 125.
Mizuno T. 1995. Bioactive biomolecules of mushrooms: food function and medicinal effect of mushroom fungi. Food Reviews International, 11(1): 5–21.
Oei P. 2003. Mushroom cultivation: appropriate technology for mushroom growers. Backhuys Publishers, Netherlands, pp: 50- 65. Pathmashini L, Arulnandhy V, Wijeratnam RS. 2009. Cultivation of oyster mushroom (Pleurotus ostreatus) on sawdust. Ceylon Journal of Science (Biological Sciences), 37(2).
Sufer O, Bozok F, Demir H. 2016. Usage of Edible Mushrooms in Various Food Products. Turkish Journal of Agriculture-Food Science and Technology, 4(3): 144–149.
Thepa S, Kirtikara K, Hirunlabh J, Khedari J. 1999. Improving indoor conditions of a Thai-style mushroom house by means of an evaporative cooler and continuous ventilation. Renewable energy, 17(3): 359–369.
Turfan N, Karadeniz M, Unal S. 2016. Comparison of some chemical contents of Ganoderma lucidum (Curtis) P. Karst collected from nature and cultured on orange stump. Turkish Journal of Agriculture-Food Science and Technology, 4(3): 158–162.