Sebahat AKSARAY, Serap SÜZÜK, Havva AVCIKÜÇÜK, Banu KAŞKATEPE

Kırıkkale Yüksek İhtisas Hastanesine on aylık süreçte başvuran hastaların gaita örneklerinden izole edilen mikrobiyota elemanı Escherichia coli izolatlarının antibiyotik duyarlılıkları

Amaç: Antibiyotik direnci, tüm dünyada global bir halk sağlığı sorunu haline dönüşmüştür. Bağırsak florası bakterileri, insan sağlığı adına birçok önemli görevi yerine getirirler. Mikrobiyota üyesi olarak Escherichia coli birçok enfeksiyona neden olmaktadır. Bu çalışmada mikrobiyota üyesi E. coli izolatlarının tedavide kullanılan antibiyotiklere karşı antibiyotik duyarlılıklarını belirlemeyi amaçladık. Yöntemler: Kırıkkale Yüksek İhtisas Hastanesi, Mikrobiyoloji Laboratuvarına Mart-Aralık 2013 tarihleri arasında gaitada gizli kan araştırılması için gönderilen ve "negatif" sonuç çıkan 150 gaita örneği değerlendirmeye alındı. E. coli izolatlarının antibiyotik duyarlılık testi Kirby Bauer disk diffüzyon yöntemiyle yapıldı ve sonuçlar Clinical Laboratory Standards Institute (CLSI) standardlarına göre değerlendirildi. Bulgular: Yüz elli E. coli izolatının 70 tanesi çalışılan tüm antibiyotiklere duyarlı bulundu. İzolatların 24 (%16)'ünde genişlemiş spektrumlu betalaktamaz saptandı. İzolatların tümü karbapenemlere duyarlı bulundu. Sonuç: Mikrobiyota elemanı E. coli izolatlarının direnç profillerinin özellikle spesifik hasta gruplarında izlenmesinin ve enfeksiyon ajanlarına karşı ampirik tedavinin belirlenmesinde önemli olacağı kanısına varılmıştır.

Antibiotic susceptibility of microbiota members Escherichia coli strains isolated from stool samples of patients attended Kırıkkale Yüksek İhtisas Hospital in ten months

Objective: Antibiotic resistance has turned into a global public health problem in all over the world. Intestinal flora bacteria perform many important functions for human health. As a member of microbiota Escherichia coli cause many infections. This study was aimed to determine antibiotic susceptibility of microbiota member E. coli isolates against to the antibiotics that used in treatment.Methods: One hundred and fifty stool samples, which were sent to Kırıkkale Yüksek İhtisas Hospital microbiology laboratory in a period of between March to December 2013 to study fecal occult blood test and were determined as "negative", were included in this study. E. coli isolates were performed antibiotic susceptibility test by the Kirby Bauer disk diffusion method according to the recommendations of the Clinical Laboratory Standards Institute (CLSI).Results: A total of 70 out of 150 E. coli isolates were sensitive to all antibiotics that used in the study. Twenty four (16%) isolates were found to be ESBL producers. All isolates were found sensitive to carbapenems. Conclusion: It was reached the conclusion that monitoring to resistance profiles of microbiota member E. coli isolates, especially in specific group patients in addition to infection agents, is important for giving direction to empirical therapy.

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