Nesrin ÇAKICI, Yasemin NUMANOĞLU ÇEVİK, Serap SÜZÜK YILDIZ, ALPER AKÇALI, NÜKHET NİLÜFER ZORBA

Gıda çalışanlarından izole edilen Enterobacterales suşlarında antimikrobiyal direnç ve GSBL/Karbapenemaz varlığının araştırılması

Amaç: Bu çalışma gıda endüstrisi çalışanlarından elde edilen toplum kaynaklı Enterobacterales üyelerinde antimikrobiyal direnç ve Genişlemiş Spektrumlu Beta Laktamaz (GSBL), karbapenemaz üretme durumunun belirlenmesi amacıyla gerçekleştirilmiştir. Yöntem: Çanakkale il merkezi ve ilçelerindeki hastaneler (n: 9) ile gıda işletmelerinde (n: 17) görevli gıda çalışanlarının (n: 300) el sürüntü örnekleri Brain Heart Infusion Broth (BHI) besiyerine alındı. İnokulümler 370 C de 24 saatlik inkübasyondan sonra Eosine Methylen Blue agar (EMB) besiyerine ekildi. Bakterilerin tanımlanmasında klasik identifikasyon ve Matriks aracılı lazer desorpsiyon iyonizasyon uçuş süresi kütle spektrometrisi (MALDITOF MS) yöntemi kullanıldı. Sefotaksim, seftazidim, meropenem ve ertapenem antibiyotiklerine karşı direnci ölçmek için disk difüzyon ve minimal inhibitör konsantrasyon (MİK) yöntemleri uygulandı ve EUCAST 2020’ye göre değerlendirildi. Tarama testi sonuçlarına göre GSBL ve karbapenemaz fenotipik doğrulama testleri uygulandı. GSBL pozitif bulunan izolatlarda polimeraz zincir reaksiyonu (PZR) yöntemine göre CTX-M geni araştırıldı. Bulgular: Elde edilen 222 adet gram negatif bakterinin tür bazında dağılımı; 129 (%58.1) Klebsiella pneumoniae, 32 (%14.4) Enterobacter cloacae, 31 (%13.9) Acinetobacter baumannii, 11 (%4.9) Escherichia coli, 8 (%3.6) Enterobacter asburiae, 4 (%1.8) Escherichia hermanni, 3 (%1.4) Enterobacter aerogenes 2 (%0.9) Klebsiella oxytoca, 2 (%0.9) Enterobacter cancerogenus olarak belirlendi. Enterobacterales üyelerinden (n: 191) 7 (%3.7)’sinin klinik sınır değerlere göre sefotaksime dirençli (

Investigation of antimicrobial resistance and ESBL/Carbapenemase presence in Enterobacterales strains isolated from food workers

Objective: This study was carried out to determine the antimicrobial resistance and the production status of ESBL, carbapenemase in community-acquired Enterobacterales members obtained from food industry workers. Methods: Hand swab samples of food workers (n: 300) working in hospitals (n: 9) and food businesses (n: 17) in Çanakkale city center and districts were taken into Brain Heart Infusion Broth (BHI) medium. Inoculum was inoculated into EMB medium after 24 hours of incubation at 37o C. Classical identification and MALDI-TOF MS method were used to identify bacteria. Disk diffusion and minimal inhibitory concentration (MIC) methods were applied to measure resistance against cefotaxime, ceftazidime, meropenem and ertapenem antibiotics and were evaluated according to EUCAST 2020. ESBL and carbapenemase phenotypic confirmation tests were performed according to the screening test results. The CTX-M gene was investigated in ESBL positive isolates using the PCR method. Results: Distribution of 222 gram-negative bacteria on the basis of species; 129 (%58.1) Klebsiella pneumoniae, 32 (14.4%) Enterobacter cloacae, 31 (13.9%) Acinetobacter baumannii, 11 (4.9%) Escherichia coli, 8 (3.6%) Enterobacter asburiae, 4 (1.8%) Escherichia hermanni, 3 (1.4%) Enterobacter aerogenes 2 (0.9%) Klebsiella oxytoca, 2 (0.9%) Enterobacter cancerogenus. It was determined that 7 (3.7%) of the Enterobacterales members (n: 191) were resistant to cefotaxime (

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