Murat HÖKELEK, Murat GÜNAYDIN, İbrahim Çağatay ACUNER, Mehmet Cihan EKMEN, Ali Serkan HEPSERT, Keramettin YANIK

Gastroduedonal yakınması olan hastalarda stool-antijen ELISA yöntemiyle Helicobacter pylori pozitifliğinin retrospektif olarak değerlendirilmesi

Amaç: Helicobacter pylori, insan gastrik mukozasında yerleşen spiral, mikroaerofilik, Gram-negatif bir bakteridir. H. pylori, gastrit, peptik ülser, mide adenokarsinomu ve B hücre lenfoması ile ilişkilidir. Yöntem: Bu çalışmada 2003-2004 yılları içerisinde çeşitli polikliniklerden gastroduedonal yakınması olan ve laboratuvara gönderilen hastaların gaita örneklerinden stool antijen (HpSA) ELİSA yöntemi ile H. pylori pozitifliği retrospektif olarak araştırılmıştır. Bulgular: Erişkin grubunda 523 erkek hastanın 410'unda (% 78,4), 856 kadın hastanın 672'sinde {% 78,5) HpSA pozitif olarak saptanmıştır. Pediyatrik grupta ise 815 hastadan 648'inde {% 79,5) pozitiflik belirlenmiştir. Sonuç: Gastroduedonal yakınması olan tüm yaş gruplarında H. pylori antijen pozitifliğinin birbirine yakın oranda olduğu ve HpSA ELİSA testinin, gastrik mukozada H. pylorfye bağlı süregelen aktif enfeksiyonun varlığı veya yokluğunu saptamaya yönelik ucuz ve uygulanabilir bir yöntem olarak kullanılabileceği düşünülmüştür.

Retrospective evaluation of Helicobacter pylori prevalence by the stool-antigen ELISA method in the patients with gastroduodenal complaints

Objective: Helicobacter pylori are microaerophilic gram-negative spiral bacteria that can be isolated from gastric mucosa in humans. H. pylori have been associated with gastritis, peptic ulcers, gastric adenocarcinoma and B-cell lymphomas. Method: In the present work, with the use of ELISA method, seroprevalence of H. pylori surface antigen (HpSA) in the stool samples of outpatients with gastroduodenal complaints have been investigated retrospectively for the period of 2003-2004. Results: In the adult group, 410 out of 523 male patients (78,4 %), and 672 out of 856 female patients (78,5 %) found to be positive in HpSA testing. In the pediatric group, 648 out of 815 patients (79,5 %) tested positive in HpSA testing. Conclusion: It is concluded that H. pylori seroprevalence rate in all investigated age groups with gastroduodenal complaints is similar, and HpSA ELISA is a convenient and inexpensive method to detect the presence or absence of an ongoing active infection caused by H. pylori in gastric mucosa.

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