Süleyman DEMİR, Hülya AYBEK, Vefa ÖZCAN, Derviş VERDİ, Ahmet BALTALARLI, Gökhan ÖNEM, İbrahim GÖKŞİN, Mustafa SAÇAR

Vitamin C iloprost attenuate skeletal muscle injury caused by ischemia-reperfusion of the lower extremities

Amaç: Sıçanda uygulanan alt ekstremite iskemi-reperfüzyonuna (İ/R) bağlı gelişlen iskelet kası hasarı üzerine iloprost ve C vitaminin etkisi değerlendirildi. Çalışma planı: Otuz dört adet Wistar albino cinsi sıçan beş gruba ayrıldı. Bir grupta (I/R, n=6), renal arterlerin altından abdominal aorta kros-klemp kondu; üç saatlik iskemi süresini bir saatlik reperfüzyon izledi. Bir gruba (n=8), kros-klemp konmadan önce juguler ven yoluyla 100 mg/kg askorbik asit verildi. Bir başka gruba (n=8) krosklemp süresince juguler ven yoluyla 20/ng/kg/min dozunda iloprost infüzyonu uygulandı. İloprost ve vitamin C gruplarında üç saatlik iskemi ve bir saatlik reperfüzyon uygulandı. Sham grubunda (n=6) sıçanlara anestezi uygulandı, batın ve juguler venöz hat açıldı; sıçanlar aynı süre boyunca izlendi. Kontrol grubunda (n=6), sternotomi sonrasında kan örnekleri alındı ve ardından soleus kasları çıkartıldı.Bulgular: İloprost ve C vitamini alan gruplarda arteryel pO2 ve HCO3 düzeyleri I/R grubuna göre anlamlı derecede yüksek iken, iskelet kası malondialdehit (MDA), plazma MDA, laktik dehidrogenaz (LDH) ve kreatin fosfokinaz (CPK) seviyeleri düşüktü (p

Vitamin C ve iloprostun alt ekstremite iskemi reperfüzyonuna bağlı iskelet kası hasarında koruyucu etkisi

Background: We evaluated the effect of iloprost and vitamin C on the reperfusion injury to the skeletal muscle, using an ischemic revascularized hindlimb model in rats. Methods: Thirty-four Wistar albino rats were divided into five groups. Three groups were assigned to ischemic/reperfusion (I/R) injury via cross-clamping of the abdominal aorta just below the renal arteries for three hours, followed by one hour of reperfusion. Of these, one group (n=8) of rats received 100 mg/kg ascorbic acid via the left jugular vein before cross-clamping, while another group (n=8) received 20/ng/kg/min iloprost by constant intravenous infusion via the left jugular venous cannula during cross-clamping. In the sham group (n=6), the rats were anesthetized, a left jugular venous cannula was inserted and the abdomen was left open during the same period. In the control group (n=6), the soleus muscles were removed and blood samples were taken immediately after sternotomy without any further treatment.Results: Compared to the I/R group, arterial blood pO2 and HCO3 levels were significantly high (p

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