Mehmet KABALCI, İbrahim Deniz CANBEYLİ, Erdinç EROĞLU

Mezenkimal kök hücrelerin greft enfeksiyonlarında antibakteriyel etkisi: Deneysel bir çalışma

Amaç: Bu çalışmada, sıçan modelinde, tigesikline kıyasla, mezenkimal kök hücrelerin metisiline dirençli Staphylococcus epidermidis ile ilişkili greft enfeksiyonu üzerindeki antibakteriyel etkileri araştırıldı. Çalışma planı: Toplam 42 erkek erişkin Wistar cinsi sıçan (yaş >6 ay; ağırlık 300-350 g) her grupta yedi sıçan olacak şekilde altı gruba ayrıldı. Grup 0 herhangi bir prosedürden geçmedi; Grup 1 enfekte edildi, ancak tedavi edilmedi; Grup 2 enfekte edildi ve greft yerleştirilmeden tigesiklin ile tedavi edildi; Grup 3 enfekte edildi ve greft yerleştirilmeden mezenkimal kök hücre ile tedavi edildi; Grup 4 greft yerleştirildikten sonra enfekte edildi ve tigesiklin ile tedavi edildi; Grup 5 greft yerleştirildikten sonra enfekte edildi ve mezenkimal kök hücre ile tedavi edildi. Oluşturulan cepler boş bırakıldı veya Dacron greftle implante edildi. Tedaviye 48 saat sonra başlandı. Numuneler 13. günde toplandı. Perigreft dokular histopatolojik olarak değerlendirildi ve bakteri koloni sayıları tespit edildi. Bulgular: Grup 0'da bakteri kolonizasyonu gözlenmez iken, Grup 1'de belirgin kolonizasyon görüldü. Grup 2 ve Grup 3'te (greftsiz gruplar) tam eradikasyon sağlandı ve Grup 4 ve Grup 5'te (greft yerleştirilen gruplar) tama yakın eradikasyon elde edildi. Histopatolojik bulgular açısından Grup 1-Grup 2 ve Grup 1-Grup 3 arasında (greftsiz gruplar) anlamlı bir fark vardı. Histopatolojik bulgular Grup 2-Grup 3 ve Grup 4-Grup 5 arasında benzerdi. Sonuç: Çalışma sonuçlarımız, mezenkimal kök hücrelerin antibiyotik tedavisine yeni ve modern bir alternatif olabileceğini ve enfekte greft alanlarında Staphylococcus’un biyoyükünü azaltabileceğini ve mezenkimal kök hücre tedavisinin tigesiklin kadar etkili olabileceğini göstermektedir.

The antibacterial effect of mesenchymal stem cells on graft infections: An experimental study

Background: In this study, we aimed to investigate the antibacterialeffects of mesenchymal stem cells, compared to tigecycline, on graftinfection related with methicillin-resistant Staphylococcus epidermidisin a rat model.Methods: A total of 42 male adult Wistar rats (age >6 months; weight300 to 350 g) were divided into six groups including seven rats in each.Group 0 did not undergo any procedure; Group 1 was infected, butuntreated; Group 2 was infected and treated with tigecycline withoutgraft placement; Group 3 was infected and received mesenchymal stemcells without graft placement; Group 4 was infected and treated withtigecycline after graft placement; Group 5 was infected and treatedwith mesenchymal stem cells after graft placement. The pocketscreated were either left empty or implanted with Dacron grafts.Treatment was commenced at 48 h. Specimens were collected on Day13. Perigraft tissues were evaluated histopathologically and bacterialcolony numbers were counted.Results: No bacterial colonization was observed in Group 0, whereasthere was a significant colonization in Group 1. Complete eradicationwas achieved in Group 2 and Group 3 (graft-free groups), andnear-complete eradication was achieved in Group 4 and Group 5(graft-implanted groups). The histopathological findings significantlydiffered between Group 1-Group 2 and between Group 1-Group3 (graft-free groups). The histopathological findings were similarbetween Group 2-Group 3 and between Group 4-Group 5.Conclusion: Our study results suggest that mesenchymal stem cellsmay be a novel, contemporary alternative to antibiotherapy and maydecrease the bio-burden of Staphylococcus at the infected graftareas, and mesenchymal stem cell treatment may be as effective astigecycline.

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