Mehmet GAMSIZKAN, Onur GENÇ, Kuthan KAVAKLI, Yusuf Sinan ŞİRİN, Hasan ÇAYLAK, Ersin SAPMAZ

Akciğer naklinde proantosiyanidinin iskemi-reperfüzyon hasarını önleyici etkisinin araştırılması: Deneysel çalışma

Amaç: Bu çalışmada proantosiyanidinin akciğer nakli sonrasında iskemi-reperfüzyon hasarını önleyici etkisi araştırıldı. Çalışma planı: Çalışmaya 12 domuz (ağırlık, 35±5 kg) dahil edildi ve domuzlar dört gruba ayrıldı. Grup 1 ve 3 kontrol grupları olarak belirlendi ve sol üst lobektomi uygulandı. Grup 2 ve 4 nakil grupları olarak belirlendi ve sol alt lobektomi ve heterotransplantasyon uygulandı. Proantosiyanidin sadece grup 3 ve 4’e verildi. Doku örnekleri ışık mikroskopu altında incelendi ve histopatolojik sonuçlar kaydedildi. Bulgular: Kontrol grupları arasında konjesyon (p=0.565), alveolar ödem (p=0.197) ve peribronşiyal enflamasyonu (p=0.444) içeren histopatolojik sonuçların sayısal değerleri açısından istatistiksel olarak anlamlı farklılık yoktu. Fakat akut hücresel reddin sayısal değerleri nakil grupları arasında istatistiksel olarak anlamlı şekilde farklı idi (p=0.048). Ortalama oksidatif stres enzimi düzeyleri grup 2’de grup 4’e kıyasla daha yüksek idi, fakat farklılık istatistiksel olarak anlamlı değildi (p>0.05). Sonuç: Deneysel çalışmamızın bulgularına göre, mobidite ve mortaliteye yol açabilen iskemi-reperfüzyon hasarını önleyici etkisine dayanarak proantosiyanidin akciğer kanserinde güvenle kullanılabilir.

Investigation of the preventive effect of proanthocyanidin in ischemia-reperfusion injury in lung transplantation: An experimental study

Background: This study aims to investigate the preventive effectof proanthocyanidin against ischemia-reperfusion injury afterlung transplantation.Methods: The study included 12 swines (weighing 35±5 kg)and separated into four groups. Groups 1 and 3 were identifiedas control groups and left upper lobectomy was performed.Groups 2 and 4 were identified as transplantation groupsand left lower lobectomy and heterotransplantationwere performed. Proanthocyanidin was only given togroups 3 and 4. Tissue samples were analyzed under lightmicroscope and histopathological findings were recorded.Results: There was no statistically significant differencebetween control groups in terms of the numerical values ofhistopathological findings that include congestion (p=0.565),alveolar edema (p=0.197) and peribronchial inflammation(p=0.444). However, numerical values of acute cellular rejectionwere statistically significantly different between transplantationgroups (p=0.048). Mean oxidative stress enzyme levels werehigher in group 2 compared to group 4; however, the differencewas not statistically significant (p>0.05).Conclusion: According to the findings of our experimental study,proanthocyanidin can be safely used in lung transplantationbased on its preventive effect in ischemia-reperfusion injury thatmay lead to morbidity and mortality.

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