Soji FAKOYA, Sandra Folarin OLOKETUYİ

Mantarlar, Lenzites Betulinus ve Coriolopsis Gallica Ekstrelerinin Antimikrobiyal Etkinlikleri ve Fitokimyasal Analizi

AMAÇ: Bakterilerin yaygın görülen çoklu ilaç dirençleri yeni antibakteriyel türleri ve direnç mekanizmasını baskılayan bileşikleri geliştirmeyi gerekli kılar. Bundan dolayı bu çalışma, Lenzites betulinus ve Coriolopsis gallica ekstrelerinin tıbbi önemi olan bazı bakteri izolatlarına karşı antimikrobiyal etkilerini değerlendirmek için gerçekleştirildi. YÖNTEM: Çalışılan mikroorganizmalar Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris, Klebsiella pneumoniae ve Escherichia coli'dir. Agar difüzyon testi kullanılarak C. gallicanın etanol, petrol eteri ve su ekstreleri bakteriyel izolatlara karşı denendi. BULGULAR: C. gallica and L. betulinusun petrol eteri ekstresi P. Vulgarisi inhibe etmekte başarılıydı (inhibisyon zonları sırasıyla 26,0 mm ve 20,0 mm). Ayrıca; L. Betulinusun su ve petrol eteri ekstreleri P. aeruginosayı sırasıyla 20,0 mm ve 23,0 mm.lik inhibisyon zonları ile inhibe ederken, C. gallicanın etanol ekstresi 22,0 mm.lik inhibisyon zonu ile P. vulgarisi inhibe etti. L. Betulinusun su ve etanol ekstreleri tarafından E. coli ve S. Aureus inhibe edildi. E. coli için inhibisyon zonu 15,0 mm ve 26,0 mm iken, S. aureus için 19,0 mm ve 22,0 mm idi. Bu çalışma aynı zamanda mantarlardaki taninler, fenolikler, flavonoidler, steroidler ve saponin gibi antimikrobiyal etkileri ile bakteriyel izolatlara karşı kullanılan fitokimyasalların varlığını gösterdi. SONUÇ: C. gallicanın etanol, su ve petrol eteri ekstrelerinde fenolikleri, flavonoidleri ve steroidleri içeren aktif komponentler bulunur. L. Betulinustan ise phenolikler, taninler, flavonoidler ve saponin elde edilir.

Antimicrobial Efficacy and Phytochemical Screening of Mushrooms, Lenzites Betulinus, and Coriolopsis Gallica Extracts

AIM: The spread of multidrug-resistant strains of bacteria makes it necessary to discover new classes of antibacterial and compounds that inhibit these resistant mechanisms. Hence, this study was conducted to evaluate the antimicrobial activities of Lenzites betulinus and Coriolopsis gallica extracts against some bacterial isolates of medical importance. METHOD: The organisms are Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris, Klebsiella pneumoniae, Escherichia coli. Using agar well diffusion assay, the ethanolic, petroleum ether and aqueous extract of C. gallica was assayed against the bacterial isolates and the result showed that Petroleum ether extract of C. gallica and L. betulinus was able to inhibit P. vulgaris with inhibition zones of 26.0 mm and 20.0 mm respectively. RESULTS: Ethanolic extract of C. gallica also inhibited P. vulgaris with inhibition zone of 22.0 mm while aqueous extract and petroleum ether of L. betulinus inhibited P. aeruginosa with inhibition zones of 20.0 mm and 23.0 mm respectively. E. coli and S. aureus were also inhibited by the aqueous and ethanolic extract of L. betulinus with zones of inhibition 15.0 mm and 26.0 mm against E. coli while 19.0 mm and 22.0 mm against S. aureus respectively. This study also showed the presence of some phytochemicals like tannins, phenolics, flavonoids, steroids, and saponin in the mushrooms with antimicrobial effects against the bacterial isolates used. CONCLUSION: The active components present in ethanolic, aqueous and petroleum ether extracts of C. gallica include phenolics, flavonoids, and steroids while phytochemicals obtained from L. betulinus are phenolics, tannins, flavonoids, and saponin respectively.

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1. Hatvani N. Antibacterial effect of the culture fluid of Lentinus edodes mycelium grown in submerged liquid culture. Int J Antimicrob Agents. 2001; 17(1): 71-74.
2. Wu CL, Chien SC, Wang SY, Ku YH, Chang ST. Structure-activity relationship of cadinane-type sesquiterpene derivatives against wood-decay fungi. Holzforschung. 2005; 59: 620-627.
3. Borchers AT, Keen CL, Gershwin ME. Mushrooms, tumors, and immunity: an update. Exp. Biol. Med. (Maywood). 2004; 229(5): 393- 406.
4. Smirnoff N, Conklin PL, Loewus FA. Biosynthesis of Ascorbic Acid in Plants: A Renaissance. Annual Review of Plant Physiology and Plant Molecular Biology. 2001; 52: 437-467.
5. Zhang M, Cui SW, Cheung PCK, Wang Q. Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity. Trends in Food Sci. & Tech. 2007; 18(1): 4-19.
6. Thekkuttuparambil AA, Kainoor KJ. Indian Medicinal Mushrooms as a Source of Antioxidant and Antitumor Agents. J Clin Biochem Nutr. 2007; 40(3): 157-162.
7. Singh J, Aneja KR. From Ethnomycology to Fungal Biotechnology: Exploiting Fungi from Natural Resources for Novel Products. Kluwer Academic/Plenum Publishers, New York. 1999.
8. Alexopoulos CJ, Mims CW. Introductory Mycology. John Wiley & Sons. 1999, p. 10.
9. Isu RN, Onyeagba RA. Basic praticals in Microbiology. 2nd edition. Fasmen Communication, Okigwe. 2002, p. 134-143.
10. Benedict RG, Brady LR. Antimicrobial activity of mushroom metabolites. J. Pharm. Sci. 2006; 61: 1820-1822.
11. Buwa LV, van Staden J. Antibacterial and antifungal activity of traditional medicinal plants used against venereal diseases in South Africa. J. of Ethnopharmaco. 2006; 103(1): 139-142.
12. Parekh J, Chanda SV. In vitro Antimicrobial Activity and Phytochemical Analysis of Some Indian Medicinal Plants. Turk. J. Biol. 2007; 31: 53-58.
13. Zar JH. Biostatistical Analysis, Prentice-Hall, Upper Saddle River, NJ, 1984.
14. Aviram M, Dornfeld L, Kaplan M, Coleman R, Nitecki S, Hoffman A. Flavonoids inhibit low density lipoprotein oxidation and cardiovascular diseases, Studies in atherosclerotic mice and human. Drugs Exp. Clin. Res. 2002; 28: 49-62.
15. Scalbert A. Antimicrobial properties of tannin. Phytochem. 2001; 30: 3875-3883.
16. Zamilpa A, Tortoriello J, Navarro V, Delgado G, Alvarez L. Five new steroidal saponins from Solanum chrysotrichum leaves and their antimycotic activity. J. Nat. Prod. 2002; 65: 1815.
17. Madhumitha G, Saral AM. Preliminary phytochemical analysis, antibacterial, antifungal and anticandidal activities of successive extracts of Crossandra infundibuliformis. Asian Pac J Trop Med. 2011; 4(3): 192-195.
18. Farombi EO. African Indigenous Plant with Chemotherapeutic Potentials and biotechnological Approach to the production of bioactive prophylacudiestic agents. Afr. J. of Biotechnol. 2003; 2(12): 662-671.
19. Rios JL, Recio MC, Villar A. Screening methods for natural products with antimicrobial activity; a review of the literature. J. Ethnopharmacol. 1998; 23: 127-149.
20. Williams L, Wilkins S. Textbook of Microbiology. 2nd Edition, New-Delhi, India. 1997.