Emmanuel Olorunju AWE, Modupe Janet MAKINDE, Kamoru Olayemi WAKEEL, Simeon Olatunde KOLAWOLE

Antiinfammatory effects of pyrenacanthia staudtii engl. (Icacinaceae) aqueous leaf extract in rodents

GİRİŞ: Astım, romatoid artrit, kanser ve nörodejeneratif bozukluklar gibi hastalıklarda, proinflamatuar faktörlerin rolleri açısından, potansiyel ve güvenli anti-inflamatuar ajanlar için yapılan sürekli aramalar önemlidir. Nijerya'nın florası tıbbi bitkiler bakımından zengin olup, bu bitkiler geleneksel halk tıbbında çeşitli enflamatuar hastalıklar için kullanılmaktadır. Bu kapsamda Pyrenacanthia staudtii Engl. (Icacinaceae) (PSE) yapraklarının sulu ekstresinin antienflamatuar aktivitesi incelenmiştir. METOD: Antiinflamatuvar aktivite; yumurta albumin, agar-akut inflamasyon için modeli, komple Freud Adjuvanı (CFA)-indüklenmiş artrit ve sıçanlarda kronik inflamasyon için pamuk pellet granülom modelleri kullanılarak değerlendirilmiştir. BULGULAR: PSE, pençe ödemi miktarını doz bağımlı olarak azaltmıştır (100-400 mg/kg) ve bu azalış istatistiksel olarak anlamlıdır (P

Kemirgenlerde pyrenacanthia staudtii engl. (Icacinaceae) bitkisinin sıvı yaprak ekstresinin antiinflamatuar etkisi

AIM: The continued search for potential and safe anti-inflammatory agents is important, in view of the roles played by proinflammatory factors in diseases such as asthma, rheumatoid arthritis, cancer and neurodegerative disorders. The Nigerian flora is rich in medicinal plants which are employed in traditional folk medicine for various inflammatory disorders. We therefore evaluate the antiinflammatory activity of the aqueous extract of the leaves of. METHOD: The antiinflammatory activity was evaluated using egg albumin, agar-induced paw edema models for acute inflammation, complete Freud Adjuvant (CFA)-induced arthritis and cotton pellets granuloma models for chronic inflammation in rats. RESULTS: PSE, produced significant (P<0.05-0.01) and dose-dependent (100-400 mg/kg) reduction of paw edema volume in rats. In chronic inflammation (CFA-induced arthritis), the extract significantly (P< 0.05) inhibited edematous response in a dose-related manner, provoking an inhibitory effect (61%, maximum inhibition) at 400 mg/kg on day 21 same as non-steroidal anti-inflammatory drug, indomethacin (5 mg/kg). PSE, also significantly (P<0.05) and dose-dependently reduce the granuloma weight in chronic model of granuloma pouch in rats. CONCLUSION: The findings of the present study indicate that PSE possesses anti-inflammatory activity and thus lend pharmacological credence to the suggested use of the plant as a natural remedy in the treatment, management and/or control of inflammatory conditions in some rural communities of Nigeria.

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1. Daziel JM. The useful plants of West Tropical Africa. London Crown Agents for Overseas Government and Administration. London. Mill Bank, 1937, p. 45.
2. Oliver-Bever B. Medicinal Plants in Tropical West Africa. London. Cambridge University Press, 1996, p. 61.
3. Iwu MM. Handbook of African Medicinal Plants. New York. CRC Press Inc, 1993, p. 27.
4. Aguwa CN, Okunji CO. Gastrointestinal studies of Pyrenacanthia staudtii leaf extract. J. Ethnopharmacol. 1986; 5: 45-55.
5. Okunji CO, Iwu MM. Control of schistosomiasis using Nigerian medicinal plants. Annual Review of Nutrition. 1986; 6: 433-458.
6. Awe EO, Makinde JM, Olajide OA, Wakeel OK, Adeloye AO. Evaluation of analgesic, anticonvulsant and hypnotic activities of Pyrenacanthia staudtii. Afr. J. Trad. CAM. 2005; 2: 122-128.
7. Winter CA, Risley EA, Niss GW. Carageenaninduced edema in the hind paw of the rat as an assay for anti-inflammatory drug. Proc. Soc. Exp. Biol. Med. 1962; 111: 544-547.
8. Lorke D. A new Approach to Practical Acute Toxicity Testing. Archives of Toxicology. 1983; 54: 275-287.
9. Backhouse N, Delporte C, Negrete E, Salimus P, Pinto A, Aravena S, CasselsBK. Antiinflammatory and antipyretic activities of Cascuta chilensis, Cestrum parqai and Psolarea glandolosa. J. Pharmagn. 1996; 34: 53-57.
10. Ekpendu TO, Akah PA, Adesomoju AA, Okogun JI. Antiinflammatory and antimicrobial activities of the Mitracarpus scaber extracts. Int. Pharmacogn. 1994; 32: 191-196.
11. Okoli CO, Akah PA. A pilot evaluation of the antiinflammatory activity of Culcasia scandens, a traditional antirheumatic agent J. Alternative and Complementary Medicine. 2000; 6: 423-427.
12. Ahmed MM, Qureshi S, Al-bekairi AM, Rao RM. Antiinflammatory activity of Caralluma tubercullata alcoholic extract. Fitoterapia. 1993; 64: 359-362.
13. Perez GRM. Anti-inflammatory activity of Ambrasia artemisaefolia and Rheo spathacae. Phytomed. 1996; 3: 163-167.
14. Gerhard Vogel H. Drug discovery and evaluation Pharmacological assays 2nd ed. Germany. Springer Publications, 2002.
15. Ghandi MSA. The anti-inflammatory, analgesic and antipyretic activities of Nigella satire. J. Ethnopharmacol. 2001; 76: 45-48.
16. Dunnett C, Goldsmith C, Buncher CR, Tsay JY. Statistic in Pharmaceutical Industry. 2nd ed. New York. Marcel Dekkar, 1993.
17. Dumas J, Bourdon U, Remade-Volon G, Adam A. Kinins and peritoneal exudates induced by carragenin and zymosan. J. Pharmacol. 1990; 101: 418-422.
18. Raychaudhuri A, Colombo C, Pastor G, Wong M, Jeng AY. Effect of capsacin on carrageenaninduced inflammation in rat pleurisy and exudates substance P level. Agent Actions. 1991; 34: 251-253.
19. Utsunomiya I, Nagai S, Ohinishi S. Sequential appearance of IL-1 and IL-6 activities in rat carrageenan-induced pleurisy. J. Immunol. 1991; 147:1803-1809.
20. Ialenti A, Ianaro A, Moncada S, Di Roossa M. Modulation of acute inflammation by endogenous nitric oxide. Euro. J. Pharmacol. 1992; 211: 177-182.
21. Dumas J, Bourdon, Remade-Volon G. Influence of a long acting bradykinin antagonist HOE B140, on acute inflammatory reactions in the rat. Eur. J. Pharmacol. 1992; 211: 81-86.
22. Whaley K, Burt AD. Inflammation, healing and repair. Eds: MacSween RMN, Whaley K: Muir’s Textbook of Pathology. 13th ed. London. Arnold, 1996, p. 112-165.
23. Cotran RS, Kumar V, Collins T. Robins Pathological Basis of Diseases. 6th Ed. W.B, 1999.
24. Majno G, Joris L. Cells, Tissues and Disease Principles of General Pathology. Cambridge. Blackwell Science,1996, p. 429-463.
25. Rafatullah S, Al-Yahya MA, Al-said MS, Abdul Hameed KU, Taragan JS. Mossa. Gastric ulcer and cytoprotective effects of Cymopsis tetragodaba (‛Guar’) in rats. Pharm. Biol. 1994; 32: 1673-170.
26. Dahiru D, Onubiyi JA, Umaru HA. Phytochemical screening and anti-ulcerogenic effect of Moringa oleifera aqueous leaf extract. Afri. J. TCAM. 2006; 3: 70-75.
27. Devi RS, Narayan S, Vani G, Devi CSS. Gastroprotective effect of Terminalia arjuma bark extract on diclofenac sodium-induced gastric ulcer. J. Ethnopharmacol. 2007; 167: 71-83.