Murat Soner BALCIOĞLU, Emine ŞAHİN, Taki KARSLI, Yüksel AKSOY, Zafer ULUTAŞ

Türkiye'de yetiştirilen bazı yerli koyun ırklarında kalpastatin (CAST) geni polimorfzminin PCR-RFLP yöntemiyle belirlenmesi

Bu çalışmada, Türkiyede yetiştirilen 7 yerli koyun ırkında kalpastatin (CAST) gen polimorfzmi PCR-RFLP metodukullanılarak araştırılmıştır. CAST geninin M ve N allelleri frekansları; Kangal (n= 31), İvesi (n= 26), Güney Karaman(n= 23), Akkaraman (n= 21), Morkaraman (n= 34), Karayaka (n= 33) ve Karakaş (n= 22) koyun ırklarında sırasıyla 0.92-0.08, 0.59-0.41, 0.67-0.33, 0.69-0.31, 0.87-0.13, 0.86-0.14, 0.89-0.11 olarak tespit edilmiştir. Ki-kare testi CAST genibakımından Morkaraman, İvesi ve Karayaka populasyonlarının Hardy-Weinberg dengesinden önemli düzeyde (P

Determination of calpastatin (CAST) gene polimorphism in some native sheep breeds reared in Turkey by PCR-RFLP method

In this study, calpastatin (CAST) gene polimorphism was investigated in 7 native sheep breeds reared in Turkey byusing PCR-RFLP method. The frequencies of M and N alleles of CAST gene in Kangal (n= 31), Awassi (n= 26), GüneyKaraman (n= 23), Akkaraman (n= 21), Morkaraman (n= 34), Karayaka (n= 33), and Karakas (n= 22) sheep breeds weredetermined as 0.92-0.08, 0.59-0.41, 0.67-0.33, 0.69-0.31, 0.87-0.13, 0.86-0.14, 0.89-0.11 respectively. According tochi-square test, all the other populations were consistent with Hardy-Weinberg equilibrium, whereas Morkaraman, İvesiand Karayaka populations showed signifcant (P

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