İnsülin-benzeri Büyüme Faktörü Reseptör Tip 1 IGF1R Geninin Ekson 7 İçindeki Tek Nükleotid Polimorfizminin SNP Angus İneklerinin Büyüme Ölçütleri Üzerine Olan Etkileri

İnsülin benzeri büyüme faktörü-1 reseptör IGF1R sinyal yolu, doğum sonrası büyüme ve gelişmede önemli bir rol oynar. IGF1R tirozin kinaz reseptörü, IGF1R’nin otofosforilasyonunu tetikleyen IGF-I tarafından aktive edilen homodimerleri ve sonrasında meydana gelen aşağı sinyal transdüksiyonu oluşturmaktadır. Bu çalışmanın amacı, sığır IGF1R geni içinde bulunan ekzon 7 içindeki sessiz SNP rs41961336; CàT ile büyüme özellikleri arasındaki ilişkinin tanımlanması, karakterize edilmesi ve incelenmesidir. Dört farklı ırktan toplam 672 adet inek polimeraz zincir reaksiyonu-restriksiyon parça uzunluk polimorfizmi PCR-RFLP metodu kullanılarak genotiplenmiştir. Çalışılan tüm ırklar Polonya Holstein Friesian, Angus, Hereford, Limousine içinde, her üç genotip varlığı sadece Angus ırkında gözlenmiştir. CC genotipi araştırılan tüm ırklarda en sık gözlenen genotip olmuş 0.7987-0.9904 ve bunu CT genotipi takip etmiştir 0.00960.1946 . Sadece Angus inekleri ilişkilendirme analizleri için seçilmiştir. 210 günlük yaşa WWT210 düzeltilmiş sütten kesim ağırlığında, CC genotipli dişi buzağıların, CT genotipli bireylerden istatistiki olarak daha ağır oldukları +5.5 kg bulunmuştur P≤0.05 . Buna karşılık, CT genotipini taşıyan bireyler, ilk buzağılamada daha yüksek canlı ağırlık kazanmıştır +10.62 kg; P≤0.05 . Bu çalışma, IGF1R/TaiI polimorfizminin genotipleri ile doğum ağırlığı, günlük canlı ağırlık kazancı veya ilk buzağılama yaşı arasındaki ilişkiyi göstermede başarısız olmuştur

Anahtar Kelimeler:

Sığır, Genetik markörler, Tirozin kinaz, Kantitatif özellik lokusu

Effects of SNP within Exon 7 of the Insulin-like Growth Factor Receptor Type 1 IGF1R Gene on Growth Traits in Angus Cows

Insulin-like growth factor 1 receptor IGF1R signaling pathway plays a key role in the postnatal growth and development. The tyrosine kinase receptor IGF1R forms homodimers that is activated by IGF-I which trigger autophosphorylation of IGF1R and subsequent downstream signal transduction. The objectives of this study were to identify, characterize and the examination of the association between silent SNP rs41961336; CàT within exon 7 of bovine IGF1R gene and growth traits. A total of 672 cows of four breeds was genotyped using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism PCR-RFLP method. A TaiI restriction endonuclease was used. Among all breeds under study Polish Holstein Friesian, Angus, Hereford, Limousine , the presence of all three genotypes was observed only for the Angus breed. The CC genotype was the most frequent in all investigated breeds 0.7987-0.9904 followed by CT 0.0096-0.1946 . Only Angus cows were selected for association analysis. At weaning weight adjusted to 210 days of age WWT210 , statistically significant differences P≤0.05 were shownfemale calves with the CC genotype were heavier +5.5 kg than the individuals with the CT genotype. However, individuals carrying the CT genotype had significantly higher body weight at first calving +10.62 kg; P≤0.05 . The present work failed to show association between genotypes of the IGF1R/TaiI polymorphism and BWT, ADG or age at first calving

Keywords:

Beef, Genetic markers, Tyrosine kinase, Quantitative trait loci,

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