Effect of Heat Shock Treatment on Microspore Embryogenesis in Brassica oleracea Species

Sıcaklık şoku uygulamaları Brassica türlerinde mikrospor embriyogenesisi uyarmak amacıyla yaygın olarak kullanılmaktadır. Bu çalışmada, 6 Türk beyaz baş lahana (Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C), 3 Türk yaprak lahana (Karadere 077, Balkaya, Yanmaz) çeşit ve genotipi ile 5 ticari F1 hibrid süs lahanası çeşidinde (Red Piegon, Victoria Piegon, Red Chidori, White Kamome, and Pink Kamome) yüksek sıcaklık uygulamalarının (32 °C ve 35 °C, 2 gün) mikrospor embriyogenesisi üzerine etkisi araştırılmıştır. Mikrospor kökenli embriyo oluşumu genotip ve yüksek sıcaklığa göre farklılık göstermiştir. En yüksek embriyo oluşumu 32 °C'de 9.92 embriyo petri-1 değeri ile Yalova-1 çeşidinden, 11.13 embriyo petri-1 değeri ile Pink Kamome F1 çeşidinden ve 35 °C'de ise 5.63 embriyo petri-1 Karadere 077 çeşidinden elde edilmiştir.

Brassica oleracea Türlerinde Sıcaklık Şoku Uygulamalarının Mikrospor Embriyogenesis Üzerine Etkisi

Heat shock treatments are widely used to induce microspore embryogenesis in Brassica species. In this study, the effect of high temperature treatment (32 °C and 35 °C for 2 days) on microspore embryogenesis was investigated in six genotypes of Turkish white head cabbage (Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C), three genotypes of Turkish kale (Balkaya, Yanmaz, Karadere 077) and five commercial F1 ornamental kale hybrids (Red Piegon, Victoria Piegon, Red Chidori, white Kamome, and Pink Kamome). Microspore-derived embryos formation differed depending on genotype and high temperature. The highest embryo yield was obtained as 9.92 embryo per petri dish in cv. Yalova-1, 11.13 embryo per petri dish in Pink Kamome F1 at 32 °C, and 5.63 embryo per petri dish in cv. Karadere 077 at 35 °C

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  • Yayın Aralığı: Yılda 4 Sayı
  • Yayıncı: Halit APAYDIN
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