Ballarda Amitraz Varroaset Kalıntısının Yüksek Performans Likit Kromatografi Yöntemi HPLC ile Belirlenmesi

Amitraz Varroaset bal arılarında Apis mellifera L. Hymenoptera: Apidae sorun olan Varroa destructor Anderson & Trueman Acarina: Varroidae „e karşı kullanılan bir akarisittir. Ballardaki amitraz kalıntısı HPLC ile tespit edilmiştir. Bal örnekleri arı yetiştiricilerinden Ankara‟nın ilçeleri olan Ayaş, Kızılcahamam ve Polatlı‟dan hasat dönemi boyunca toplanmıştır. Toplanan 32 örnekten 6 %15 adedinde amitraz kalıntısına rastlanılmıştır. Örneklerde tespit edilen kalıntı miktarları sırasıyla 5.35, 0.34, 0.23, 1.27, 0.92 ve 0.40 ppm. dir.Tespit edilen kalıntı limiti 0.0643 ppm ve elde edilme oranı %56,71 dir. Bu sonuç doğrultusunda Dünya Sağlık Örgütü WHO ‟ nün izin vermiş olduğu limit 1 mg/kg iken bazı bal örneklerinde daha yüksek düzeyde amitraz kalıntısı olduğu görülmektedir

Anahtar Kelimeler:

Amitraz, kalıntı, HPLC, bal, Varroa destructor, Apis mellifera

Determination of Amitraz Varroaset Residue in Honey by High Performance Liquid Chromatography HPLC

Amitraz Varroaset is an acaricide applied against Varroa destructor Anderson & Trueman Acarina: Varroidae infestations on honeybees Apis mellifera L. Hymenoptera: Apidae . Amitraz residue in honey was determined by HPLC in Ankara, Turkey. Honey samples were collected from beekeepers during the harvesting period in Ayaş, Kızılcahamam and Polatlı towns in Ankara. Amitraz residue was found in various levels in six 15% out of 32 honey samples. The residue amounts in the 6 samples were 5.35, 0.34, 0.23, 1.27, 0.92 and 0.40 ppm. The limit of detection was 0.0643 ppm and the recovery ratio was 56.71 %. According to the results, some honey samples showed higher residue levels of amitraz than the World Health Organisation WHO limit of 1 mg/kg

Keywords:

Amitraz, residue, HPLC, honey, Varroa destructor, Apis mellifera,

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Abbed, T. and J. Ducos de Lahıtte. 1993. Detemination de la DL 50 del‟ amitraz et du coumaphus sur Varroa jacobsoni Oudemans au moyen des acri des antivarroa (Schering) et perizin (Bayer). Apidologie, 24: 121-126.
Anonymous 2002. Turkish Alimentarous codex (28.04.2002 date; no:24739;002/30).
Atianza, J., J. J. Jimenez, J. L. Bernal and M.T. Martin. 1993. Supercritical fluid extraction Of fluvalinate residues in honey. Determination by high performance liquid chromatography. Biomedical Application, 655 (1): 95-99. of Chromatogfraphy
Aydın, L., İ. Çakmak, E.Gülegen and M. Korkut. 2003. Reports of The South Marmara Regions. Honey bee pests and diseases. Uludağ Beekeeping Bulletin, 3 (1): 37-40.
Baxendale, P.F. and D.L. Keith. 1993. Avoiding honey bee losses when using insecticides. G93-File:1174 - A. Insects and Pests, University of Nebraska-Lincoln. Pubs@unl.edu
Berzas Nevado, J. J. M. V. Maherdero. Olibares, J.A. and F. Salinas. 1991. Determination of amitraz in honey by first-derivative spectrophotometry. International Journal of Environmental Analytical Chemistry. 43:187-194.
Bulakeri, N. and G. Tufan. 1986. Determination of pesticide residues in honey of Izmir provinces.Izmir Food Control and Research Institute report(1985), pp 34-48.
Cabras, P., M. Melis and I. Spanedda. 1993. Detemination of Cymaizol residues in honey by liquid chromatography. J.A.O.C.I., 76 (1): 92-94. 174
TARIM BİLİMLERİ DERGİSİ 2008, Cilt 14, Sayı 2
Cavallora, R. 1989. Presents status of Varroatosis in Europe and progress in Varroa mite control. Prooceeding of meeting of the EC- expert group, Italy, 28-30 November. European community: Luxembourg,1097.
Fernandez, M. and J. Lozano. 1993. Gas Chromatographic- mass spectrometric method for the simultaneous determination of Amitraz, Bromopropylate, Coumphos, Cymiazole and fulvalinate resiudue in honey. Analyst, 118 (12): 1519-1522.
Fıratlı, Ç., F. Genç, M. Karacaoğlu and H. V. Gençer. 2000. 6th.Technical Engineery, Ankara, pp 811-826. of Turkish Agricultural
Floris,I., A. Satta, V. L. Garau, M. Melis, P. Cabras and N. Aloul. 2001. Effectiveness, persistence, and residue of amitraz plastic strips in the apiary control of Varroa destructor. Apidologie (32): 577-585
Garcia, M.A., M.I. Fernandez, L.C. Herrero and M.J. Melgar. 1996. Acaricide residue determination in honey. Bull. Environ. Contam.Toxicol., 56: 881-887
Greef, M. D., L. D. Wael and O. Laere. 1994. The determination of the Fulvalinate residues. In The Belgian Honey and Beeswax. Apiacta. 29 (4): 83-87.
Hammerling, B. and C.H. Augustyniak, Risto. 1991. Gesammt-Amitraz Rückstande in Bienenhonigen. Die Nahrunk, 35 (10):1047-1052.
Imdorf, A., S. Bogdanov,V. Kilehenmann and C. Maquelin. 1995. A new Varroacide with Thymol as the mainiIngredient. Bee World, 76 (2): 77-83.
Kaftanoğlu O., U. Kumova and H. Yeninar. 1992. Recent development of Varroa control 1. National Beekeping Seminar of Eastern Anatolia , 127-139. Erzurum, Turkey
Kolankaya, D., O. Koçak, K. Sorgun and B. Erkmen. 2001. Important parasite Varroa and control in beehives. Beekeeping Technical Bulletin: 74 : 25-29.
Korta, E., A .Bakkali, L A. Berrueta, B. Gallo, F. Vicente, V. Kilchenmann and S. J. Bogdanov. 2001. Study of acaricide stability in honey. Characterization of amitraz degradation products in honey and beeswax. Agric- Food-Chem. 49(12): 5835-42
Martel, A.C. and B. S. Zeggane. 2002. Determination of acaricides in honey by high-performance liquid chromatography with photodiode array detection. Journal of chromatography A, 954 (1-2): 173-180.
Özbek, H. 1990. Honey bee (Apis mellifera L.) stinging. Journal of Agricultural Faculty of Atatürk University, 21 (2): 84-100.
Pass, M, A. and T. D. Mogg. 1991. Effect of amitraz and its metobolites Biochem.phystol, 99 (12): 169-172. motility. Comp.
Rutner, F. 1988. Biogeography and taxonomy of honeybees. Springer – Verlag, Berlin. p: 293.
Sirali, R. 2002. General beekeeping structureof Turkey. Uludağ Bee Journal 4 (2): 30-39.
Stoeppler, M., U. Kurfuerst, P. Rudolph, H.A. Memkeen, P. Fuerst, W. Grobel, L. Matter and G. Frisch. 1986. Varroa disease of The Honeybee Apis mellifera. Bee World. 62 (4): 141-154.
Taccheo, M. 1988. The determination of total amitraz residues in honey by electron capture capillary gas chromatography-A Science, 23: 59-64. method. Pesticide
Tomlin, C. D. S. 2002-2003. A world compendium the pesticide manual (Twentieth Edition). Hampshire: Crop Protection Council.1344p.
Tutkun, E. and A. Inci. 1992. Balarısı zararlıları ve tedavi yöntemleri Matbaacılık, Ankara, 156p. tedaviye). Demircioğlu
Tutkun, E. and A. Boşgelmez. 2003. Balarısı zararlıları ve hastalıkları Teşhis ve tedavi yöntemleri Bizim Büro Basımevi, Ankara, 365p.
Tseng, S-H., P.C. Chang and S.S. Chou. 1999. Determination of amitraz residue in fruit by high performance liquid chromatography. Journal of Food and Drug Analysis, 7 (3): 225-232.