The efects of oxytetracyeline and flumequine on the rotifers (Brachionus plicatilis O.F.Müller)

Flumequine ve Oxytetracycline'in farklı dozlarının Brachionus plicatilis O.F. MÜLLER üzerindeki etkileri çalışıldı ve antibiyotiklerin farklı dozlarmdaki ölüm oranlan tespit edildi. Ayrıca rotiferler tarafından absorbe edilen Oxytetracycline miktarı Elisa testi ile saptandı. Her denemede 50 It'lik polietilen torbalar kullanıldı. 0,0 ppm, 25,0 ppm, 50,0 ppm, 100,0 ppm, 150,0 ppm ve 200,0 ppm'lik antibiyotik dozları torbalara eklendi. Yaklaşık beş saat sonra her gurup 45 $\mu$ m'lik membran filtreden süzülerek Elisa testiyle analiz edildi ve rotiferler tarafından absorbe edilen antibiyotik miktarı tespit edildi. Yukarıda bahsedilen antibiyotikler arasında balık larvalarının tedavisinde en uygun antibiyotiğin Oxytetracycline olduğu bulundu. Analizlere göre, 25,0 ppm, 50,0 ppm, 100,0 ppm, 150,0 ppm ve 200,0 ppm için absorbe edilen antibiyotik miktarları sırasıyla 3,58 ppb; 1,5 ppb; 3,91 ppb; 2,5 ppb ve 4,46 ppb olarak tespit edildi.

Anahtar Kelimeler:

ELISA, dozaj etkileri, Rotifera, antibiyotikler, oksitetrasiklin

Oxytetracyeline ve flumequine'in rotiferler (Brachionus plicatilis O.F.Müller) üzerindeki etkisi

The effects of different dosages of Flumequine and Oxytetracycline were studied on Brachionus plicatilis O.F. MÜLLER and the rates of death for different dosages of antibiotics were determined. In addition, the amounts of the Oxytetracycline absorbed by the rotifers were determined by Elisa test. Polyethylene bags of 50 It were used in each trial. 0.0 ppm (control group), 25.0 ppm, 50.0 ppm, 100.0 ppm, 150.0 ppm, and 200.0 ppm dosages of antibiotics were added into the bags. Approximately five hours later, each group was filtered through a 45 $\mu$ m Millipore membrane filter and analysed by Elisa test, being determined the amount of the antibiotic absorbed by the rotifers. It was found that the most suitable antibiotic on the treatment of fish larvae is Oxytetracycline between the antibiotics above. According to the analysis, antibiotic amounts absorbed for 25.0 ppm, 50.0 ppm, 100.0 ppm, 150.0 ppm, and 200.0 ppm were determined as 3,58 ppb; 1,5 ppb; 3,91 ppb; 2,5 ppb; and 4,46 ppb, respectively.

Keywords:

ELISA, dosage effects, Rotifera, antibiotics, oxytetracycline,

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