Levrek yavrularında (Dicentrarchus labraw L., 1758) vibriozise karşı aşı geliştirilmesi

Levrek yavrularında (Dicentrarchus labrax L., 1758) Vibrio anguillarum Serotip O1’in neden olduğu vibriozise karşı başarılı bir daldırma aşı geliştirildi. 0.5-3 g levrek yavruları 1:10 sulandırılmış 2x 109 cfu ml-1 bakteri içeren formalin ile inaktive edilmiş aşı ile 30 saniye süreyle daldırma yöntemiyle aşılandı. Balıklar 10-15 g ağırlığa ulaşınca 1 dakika daldırma yöntemiyle tekrar aşılandı. Korunma 21 gün ve 240 gün sonra sırasıyla banyo ve intra peritoneal enjeksiyon yöntemiyle deneysel enfeksiyon oluşturularak belirlendi. Tüm deneysel enfeksiyonlar 2 tekrar olarak düzenlendi. Aşılı (n= 30) ve kontrol grupları (n= 44) 1.23x107 cfu ml-1 patojenik V. anguillarum Serotip O1 içeren deniz suyunda 40 dakika süreyle banyo yaptırıldı. Aşılı ve kontrol grubunda sırasıyla %10 ve %59 mortalite görüldü. Hesaplanan görecel yaşam oranı (RPS) 83 bulundu. İkinci deneysel enfeksiyon intra peritoneal yolla 0,5 ml fosfat tuz tamponu içinde (PBS) (pH 7,4) 1,75 x 105 cfu ml-1 patojenik V. anguillarum Serotip O1 verilerek gerçekleştirildi. Bir kez aşılı (n= 38) iki kez aşılı (n= 40) gruplarda mortalite oranları sırasıyla %53, %27.5 olarak bulundu. Fakat aşısız grupta (n= 34) %71 mortalite bulundu. Sonuçlar, 2 kez aşılı grupta, aşılamadan 240 gün sonra iyi bir korunmanın olduğunu ortaya koydu (RPS=61.3).

Anahtar Kelimeler:

ölüm oranı, aşı geliştirme, balık yavrusu, levrek, Dicentrarchus labrax, Listonella anguillarum

Vaccine development in sea bass fry (Dicentrarchus labraw L., 1758) against vibriosis

A successful dip vaccine against vibriosis which caused by Vibrio anguillarum Serotype 01, was developed for sea bass fry. 0.5-3 g of sea bass fry were vaccinated with 1:10 diluted vaccine which, containing 2x10 9 cfu ml-' formalin inactivated V. anguillarum Serotype 01, for 30 seconds. Fish were boosted for 1 minute bath when reached 10-15 g of size. Protections were determined following 21 days and 240 days with experimental bath challenge and intraperitoneal challenges respectively. All challenges were designed two replicates. Vaccinated (n=30) and control groups (n= 44) were infected with pathogenic V. anguillarum serotype 01 (1.23x107 cfu ml ') for 40 minutes bath. 10% and 59% mortality were recorded in vaccinated and control groups respectively. Calculated relative percentage survival (RPS) was 83. The second challenge was performed by 0.5 mi intraperitoneal injection of 1.75x105 cfu ml1 pathogenic V. anguillarum serotype 01 which was diluted with phosphate buffer saline (PBS) (pH 7.4) and inoculated to one time vaccinated {n= 38), booster vaccinated (n= 40) and control groups. 53% and 27.5% mortalities were observed in one time vaccinated and boosted fish respectively. However 71% mortality were found in unvaccinated control (n= 34). The results showed that good protection was obtained in booster vaccinated group following 240 days from vaccination (RPS= 61.3).

Keywords:

mortality, vaccine development, fry, sea bass, Dicentrarchus labrax, Listonella anguillarum,

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