Biotyping of lactococcus garvieae isolated from Turkey

Alabalıklardan izole edilen 20 Lactococcus garvieae suşu fenotipik özellikleri, serolojik özellikleri ve kapsül oluşumu yönünden incelendi. İzolatlar API 20 Strep API 50 CH ve klasik bakteriyolojik yöntemlerle identifiye edildi. Suşların tamamının kapsullü olduğu, kapsullü İspanya suşuna karşı hazırlanmış antiserum ile aglutine olduğu görüldü. Sonuçlar Türkiyeden yapılan izolatların homojen ve biyotip 3 olduğunu, ve İspanyadan yapılan izolatlatlarla biribirine yüksek oranda benzediğini ortaya koydu. Klasik bakteriyolojik incelemede izolatların %40’ının laktozdan asit oluşturabildiği belirlendi.

Türkiye' den izole edilen lactococcus garvieae' ların biyotiplendirilmesi

Twenty Lactococcus garvieae strains isolated from rainbow trout were investigated in phenotypic, serologic and capsule formation. Isolates were identified with API 20 Strep, API 50CH and conventional bacteriologic techniques. All strains were capsulated and agglutinated with antiserum, which raised against capsulated Spanish strain. The results were indicated that high level of homogeneity in Turkish isolates, all were biotype 3 and high similarity between Spanish and Turkey isolates. 40% of the isolates were able to produce acid from lactose in conventional techniques

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Austin, B., D. A. Austin, 1999. Bacterial fish pathogens: Diseases of Farmed and Wild Fish, 3nd rev.ed. Praxis Publishing Ltd, Chichester, UK.

Barnes A. C, C. Guyot, I., B. G. Hansen, K. Mackenzie, M. T. Home, A. E. Ellis, 2002 Resistance to serum killing may contribute to differences in the abilities of capsulate and non-capsulated isolates of Lactococcus garvieae to cause disease in rainbow trout.(Oncorhynchus mykiss L).Fish&Shellfish Immunol. 12:155-168.

Booker, J., G. D. Jr. Imes, C. M.Cameron, T. W.Naude, H. J. Schoonbee, 1979. Trout Mortalities as a result of Streptococcus Infection. Onderstepoort. J.Vet. Res:"46,71 -77.

Bragg, R. R., J. S. E. Broere, 1986.Streptococcosis in Rainbow Trout in South Africa.Bull. Eur. Ass. Fish Pathol. 6 (3) 89-91.

Cagirgan, H., T. T. Tanrikul, 1995. A new problem Enterococcus-like Infection in Rainbow Trout (Oncorhynchus mykiss) Farms in Turkey (in Turkish). Vet. Kont. Ve Arast. Enst. Md. Derg. C. 19, S.33:9-19.

Carson, J., N. Gudkovs, B. Austin, 1993 Characteristics of an Enterococus like Bacterium from Australia and South Africa, Pathogenic for Rainbow Trout, Oncorhynchus mykiss Walbaum. J.Fish Dis. 16:381-388.

Chang, P. H., C. M. Lin, H. Plarre (2002) Lactococcus garvieae infection of cultured rainbow trout, Oncorhynchus mykiss, in Taiwan and associated biophysical characteristics and histopathology. Bull. Eur. Ass. Fish Pathol., 22(5)319-327.

Diler, Ö., S. Altun, A. K. Adiloğlu, A. Kubilay, B. Işıklı, (2002) First Occurance of Streptococcosis affecting farmed rainbow trout {Oncorhynchus mykiss) in Turkey. Bull. Eur. Ass. Fish Pathol 22(1) 21-26.

Domenech, A., J. Prieta, J. F. Ferna'ndez-Garayza'bal, M. D. Collins, D. Jones, L. Domy'nguez. 1993. Phenotypic and phylogenetic evidence for a close relationship between Lactococcus garvieae and Enterococcus seriolicida. Microbiologia 9:63-68.

Eldar, A., C. Ghittino, L. Asanta, E. Bozzetta, M. Gloria, M. Prearo, H. Bercovier, 1996. Enterococcus seriolicida is a junior synonym of Lactococcus garvieae, a causative agent of septicemia and meningoencephalitis in fish.Curr. Microbiol. 32:85-88.

Eldar, A., M. Gloria, C. Ghittino, A. Zlotkin, H. Bercovier. 1999. Biodiversity of Lactococcus garvieae strains isolated from fish in Europe, Asia, and Australia. Appl. Environ. Microbiol. 65:1005-1008.

Faciam, R., J. A. Elliott (1995). Identification, Classification, and Clinical Relevance of Catalase-Negative Gram-Positive Cocci, Excluding the Streptococci and Enterococci. Clin. Microbiol. Rew.8:479-495.

Ghittino C, M. Prearo (1993). Comparison of some strains isolated from rainbow trout affected by streptococcosis. Boll. Soc. Ital. Patol. 11:30-43.

Hirono I., H. Yamashita, C. Park, T. Yoshida, T. Aoki, 1999. Identification of genes in a KG- phenotype of Lactococcus garvieae, a fish pathogenic. Microbial Pathogen. 27:407-417.

Kim, Y. G, K. K. Lee, 1994. Isolation Characterisation and Pathogenicity of a Streptococcus in the Flounder {Paralichthys olivaceus) Cultured in Korea. Bull. Eur. Ass. Fish Pathol. 14 (1), 8-11.

Koneman, E. W., S. D. Allen, W. M. Janda, P. C. Schreckenberger, W.C. Jr. Winn, 1992. Color Atlas and Textbook of Diagnostic Microbiology Fourth Ed.p.431-466.

Kusuda, R., K. Kawai, F. Salati, C. R. Banner, J. L. Fryer, 1991. Enterococcus seriolocida sp. nov., a Fish Pathogen. Int. J. Syst. Bacteri. 41,406-409.

Mundt, J. O., 1986 Lactic acid streptococci, pp.1065-1066. In Sneath, P.H.A., Mair, N.S., Sharpe, M.E. and Holt, J.G. (Eds) Bergey's Manual of systematic Bacteriology, Vol 2.Williams and Wilkins, Baltimore.

Plumb, J. A., J. H. Schachte, J. L. Gaines, W. Peltier, and B. Carol, WA.Streptococcus sp. from marine fishes along the Alabama and North-West Florida coast of Gulf of Mexico. Trans. Am.Fish. Sod03, 358-361.

Prieta, J., A. M. Domenech, J. F. Fernandez-Grayzabal, M. D. Collins, U. M. Rodrigues, D. Jones, A. Rodrigues, L. Dominguez, 1993 Lactococcicosis de la Trucha Arco Iris (Oncorhynchus mykiss). Med. Vet. Vol 10, (6) 367- 373.

Ravelo, C, B. Magarinos, J. L. Romalde, A. E. Toranzo (2001) Conventional versus miniaturized systems for the phenotypic characterization of Lactococcus garvieae strains. Bull. Eur. Ass. Fish Pathol. 21(4) 136-144.

Salati, F., P. Tassi, P. Bronzi, 1996. Isolation of Enterococcus like Bacterium from Diseased Adriatic Sturgeon Acipenser naccarii, Farmed Italy. Bull. Eur. Ass. Fish Pathol. 16 (3) 96-99.

Sneath, P. H. A., N. S. Mair, M. E. Sharpe, J. G. Holt, 1986. Bergey's Manual of Systematic Bacteriology, Vol 2.Williams and Wilkins, Baltimore.

Teixeira, L. M., V. L. C. Merquior, M. C. E. Vianni, M. G. S. Carvalho, S. E. L. Fracalanzza, A. G. Steigerwalt, D. J. Brenner, R. R. Facklam, 1996. Phenotypic and genotypic characterization of atypical Lactococcus garvieae strains isolated from water buffalos with subclinical mastitis and confirmation of L garvieae as a senior subjective synonym of Enterococcus seriolicida. Int. J. Syst. Bacteriol. 46:664-668.

Toranzo, A. E., S. Devesa, P. Heinen, A. Riaza, S. Nun'ez, J. L. Barja, 1994. Streptococcosis in cultured turbot caused by an enterococcus-like bacterium. Bull. Eur. Assn. Fish Pathol. 14:19-23.

Toranzo, A. E., A. M. Baya, B. S. Roberson, J. L. Barja, D. J. Grimes, F. M. Hetrick (1987). Specificity of slide agglutination test for detecting bacterial fish pathogens. Aquaculture 61,2,81-97.

Toranzo, A. E., S. Devesa, P. Heinen, A. Riaza, S. Nunez, J. L. Barja, 1994. Streptococcosis in Cultured Turbot Caused by an Enterococcus-Wke Bacterium. Bull. Eur. Ass. Fish. Pathol. 14 (1) 19-23.

Vela, A. I., C. Vazquez, A. Gibello, M. M. Blanco, M. A Moreno, P. Liebana, C. Albendea, B. Alcala, A. Mendez, L. Dominguez, J. F. Fernandez- Garayzabal (2000). Phenotypic and Genetic Characterization of Lactococcus garvieae Isolated in Spain from Lactococcosis Outbreaks and Comparison with Isolates of Other Countries and Sources. J. Clin. Microb. 38,10:3791-3795.

Walbanks, S., A. J. Martinez-Murcia, J. L. Fryer, B. A. Phillips, M. D. Collins, 1990.16 S RNA Sequence Determination for Members of Genus Carnobacterium and Related Lactic Acid Bacteria and Description of Vagococcus salmoninarum sp. nov. Int. J. Syst. Bacteriol. 40,224-230.

Williams, A. M., J. L. Fryer, M. D. Collins, 1990. Lactococcus piscium sp. nov. A new Lactococcus species from Salmonid Fish. FEMS Microbiol. Lett. 68,109-114.
Su Ürünleri Dergisi-Cover
  • ISSN: 1300-1590
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 1984
  • Yayıncı: Aynur Lök