Cenker KILIÇ, TOLGA GÖKSAN, İLKNUR AK, ŞEVKET GÖKPINAR

İki farklı Spirulina platensis suşunun büyüme özelliklerinin karşılaştırılması

Mikroalgal biyoteknoloji kapsamında sıklıkla kullanılan türlerden Spirulina platensis’in üretimi son yıllarda ülkemizde de başarıyla gerçekleştirilmektedir. Bu çalışmada, düz ve spiral formda olan iki farklı Spirulina suşunun büyüme karakteristikleri incelendi. Ayrıca, klorofil ve kuru ağırlık ölçümlerinde pratiklik sağlayacak spektrofotometrik ölçümler çalışıldı. Deneme süresince, hücre sayıları haricinde tüm parametrelerin birbirine benzerlik gösterdiği saptandı. Denemenin başında spiral/düz form oranı 2.43 iken bu oran deneme sonunda 5.53’e yükseldi. Spektrofotometrik ölçümler, kültürlerde büyümenin izlenmesinde pratiklik sağlaması bakımından önemlidir. Bu kapsamda, 680 nm’de ölçülen absorbans değeri ile kuru ağırlık ve klorofil a miktarları arasında önemli bir ilişki olduğu saptandı (p

Anahtar Kelimeler:

yem zenginleştiriciler, mavi-yeşil bakteriler

Comparison of the growth characteristics in two different Spirulina platensis stains

Comparison of the growth characteristics in two different Spirulina platensis strains. The cultivation of Spirulina platensis, which is often used in microalgal biotechnology, has successfully been carried out in our country as well. In this study, the growth characteristics of two Spirulina strains in straight and spiral forms were investigated. In addition, spectrophotometric measurements, which make the measurements of chlorophyll and dry weight more practical, were studied. It was found that all the parameters except the cell count were similar during the experiment. While the spiral/straight form ratio was 2.43 at the beginning of the experiment, it increased up to 5.53 at the end. Spectrophotometric measurements are of importance in terms of practicality in the monitoring of the growth in the cultures. In this respect, a strong correlation was determined between the absorbance values at 680 nm and the amounts of dry weight and chlorophyll (p<0.05). As a result, though there was not a significant difference between the growth parameters of the both forms, the use of straight one would be better due to the problems encountered during harvesting.

Keywords:

feed supplements, cyanobacteria,

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