Doğal levrek (Dicentrarchus labrax) anaçlarında mikrosatelit polimorfizmi

Su ürünleri yetiştiriciliğinde eldeki stokların genetik yapısını tanımlamak, biyolojik çeşitliliğin korunması ve çiftliklerden doğaya kaçışın etkilerinin en aza indirilmesinin yanında, uygulanan seleksiyon yoluyla ıslah programlarının da uzun vadede yararlı sonuçlar ortaya çıkarması gibi açılardan önemlidir. Çalışmada iki kuluçkahanenin anaç stoklarının (Akvatek 149, Egemar 81 adet) genetik yapısı mikrosatelit polimorfizmi açısından karşılaştırılmış, lokuslara ait parametrelerin populasyonlara göre değiştiği gözlenmiştir. İncelenen 12 adet lokusun allel sayıları populasyonlar ayrı ayrı ele alındığında 3 ile 17 arasında değişmektedir. Akvatek için en polimorfik lokus DLA0064 ($H _G$=0.9697, $H _B$=0.8479), Egemar için DLA0070 ($H _G$=0.8333, $H _B$=0.8433) olarak belirlenmiştir. Tüm sonuçlara göre mikrosatelit polimorfizminin populasyonlara göre değiştiği söylenebilir.

Anahtar Kelimeler:

Dicentrarchus labrax, mikrosatellitler, levrek, polimorfizm, genetik farklılık

Microsatellite polymorphism of natural sea bass (Dicentrarchus labrax) breeders.

Determination of genetic structure of available aquaculture stocks is an important subject for protecting biodiversity and reducing effects of escapes from farms and also for getting good results for long term from selective breeding. In the study broodstock (Akvatek 149, Egemar 81) of two hatcheries were compared using microsatelite polymorphism, changes in parameters for loci depending on the population were observed. Allel numbers of 12 loci that were investigated were between 3 and 17 when populations considered seperately. DLA0064 was found to be the most polymorphic locus for Akvatek $H _0$=0.9697, $H _E$=0.8479) and DLA0070 was found to be the most polymorphic locus for Egemar ($H _0$=0.8333, $H _E$=0.8433). According to all results, it can be said that microsatellite polymorphism might show changes depending on the populations.

Keywords:

Dicentrarchus labrax, microsatellites, sea bass, polymorphism, genetic diversity,

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