Büyük hacimli rotifer (Brachionus plicatilis O.F.Müller,1758) kültüründeki aerobik bakteriyel floranın kantitatif tayini

Bu araştırmada besleme rejimine (alg+maya+protein selko) bağlı olarak büyük hacimli (2700 L) tanklarda üretilen rotifer ve kültür ortamlarındaki aerobik bakteriyel flora kantitatif olarak değerlendirilmiştir. Bakteri sayımlarında toplam bakteri M65 agar, Vibrio spp TCBS-Cholera Medium agar (Oxoid), Pseudomonas spp., Pseudomonas -CFC-agar (Oxoid)'da yapılmıştır. Rotiferler çeşitli alg türleri (Isochrysis galbana;Jabiti susu, Tetraselmis suecica, Nannochloropsis oculata', Droop 1955), ekmek mayası (Saccharomyces cervisiae) ve protein selko ile beslenmiştir. Rotifer ve kültür ortamlarında bakteriyel sayılar maya beslemesi ile artmasına rağmen, protein selko uygulamasından sonra maksimuma ulaşmaktadır (Toplam bakteri 92x106 kob $ml^{-1}$ ; Vibrio spp. sayısı 20 x106 kob $ml^{-1}$ ; Pseudomonas spp. sayısı 25 x105 kob $ml^{-1}$ )

Anahtar Kelimeler:

nicelik analizleri, Rotifera, patojenler, Pseudomonas, Vibrio, algler, bakteri sayısı, kültür balığı

The quantitative determination of aerobic bacterial flora in rotifer (Brachionus plicatilis O.F.Müller,1758) in large scale rotifer cultures

The quantitative determination of aerobic bacterial flora in rotifer (Brachionus plicatilis O.F. Miiller,1758) in large scale rotifer cultures. In this study, depending on the nutritional regime, quantitative analysis of aerobic bacterial flora in both culture media and rotifers grown in large scale tanks (2700 L.) was carried out. M65 agar, TCBS-Cholera Medium Agar (Oxoid) and Pseudomonas-CFC-Agar (Oxoid) were used for counting of total bacteria, Vibrio spp. and Pseudomonas spp. respectively. Protein selco, algae (Isochrysis galbana; Tahitian strain, Tetraselmis suecica', Nannochloropsis oculata, Droop 1955), and baker's yeast (Saccharomyces cervisiae) were favored as food stuff for rotifer cultures. Although bacterial counts increased in rotifer cultures fed with yeast, these numbers rearched maximum levels after protein selco treatment (total bacteria count 92x106 cfu $ml^{-1}$ ;Vibrio spp. count 20x106 cfu $ml^{-1}$ ; Pseudomonas spp. count 25x105 cfu $ml^{-1}$ ).

Keywords:

quantitative analysis, Rotifera, pathogens, Pseudomonas, Vibrio, algae, bacterial count, fish culture,

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