M.Akbar YOLDAŞ, Hikmet KATIRCIOĞLU, Yavuz BEYATLI

Bazı mavi-yeşil alglerin (Cyanophyta-Cyanobacteria) poli- $beta$ -hidroksibütirat (PHB) üretimi ve antimikrobiyal aktivitelerinin incelenmesi

İnsanların kendi yararlarına kullanmaya başladığı doğal kaynaklar arasında mikroorganizmaların çok önemli bir yeri vardır. Fotosentetik, gram negatif mavi-yeşil alglerde bu doğrultuda önemli role sahiptir. PHB'nin biyoplastik polimeri olarak önemi bilinmekte olup, karbon ve enerji kaynağı olarak bu grandilerin Cyanobacteria'da birikimi hakkında sınırlı bilgi mevcuttur. Aynı zamanda mavi-yeşil alglerin antibiyotik, algisit, farmasötik olarak aktif ve bitkilerin büyümelerini düzenleyici maddeler sentezlediği bilinmektedir. Bu çalışmada Synechocystis sp.'nin 6 farklı suşunun PHB üretim yeteneği ve antimikrobiyal aktivitesi incelenmiştir. Suşlann PHB üretimlerinin belirlenmesinde üç farklı besiortamı kullanılmıştır (BG11, Ashbey's ve Beggiatoa). Suşlann PHB üretimleri spektrofotometrik yöntemle belirlenmiş ve PHB üretim miktarları hücre kuru ağırlığına göre BG11 besiortammda %13.6-38.8, Ashbey's besi ortamında %0.7-50.6 ve Beggiatoa besiyerinde %3.8-77.5 olarak bulunmuştur. Suşlann antimikrobiyal aktiviteleri 16 farklı test bakteri üzerinde denenmiştir. Antimikrobiyal aktivite tespitinde ağar diffiizyon metodu kullanılmıştır. Yalnız Synechocystis sp. B6 susunda Micrococcus flavus (0.36 mm. zon çapı) ve Proteus vulgaris (0.35 mm. zon çapı)'e karşı antimikrobiyal etki tespit edilmiştir. Diğer suşlann test edilen bakterilere karşı antimikrobiyal etkisinin olmadığı saptanmıştır.

Anahtar Kelimeler:

Türkiye, mavi-yeşil bakteriler

Stduy on polii- $beta$ -hydroxybutrate (PHB) production and antimicrobial activities of some blue-green algae (Cyanophyta-Cyanobacteria)

Microorganisms have very important role among the natural springs that people use. On this point, photosenthetic, gram negative blue-green algae has a significant role. PHB is known as bioplastic polymer and there is limited knowledge about the accumulation of these granules by Cyanobacteria as a source of carbon and energy. Meanwhile, blue-green algae is active as antibiotic, algisit, pharmachologic and consists of elements which balance the plants growth. On this study, PHB production and antimicrobial activity of 6 different strains of Synechocystis sp. were tested. Three different medium were used in the studing of PHB production by Cyanobacteria (BG11, Ashbey's and Beggiatoa). A spectrophotometric method was used for determining PHB production by the strains. PHB production of the strains according to the cell dry weight were found 13.6-38.8% in Bull, 0.7-50.6% in Ashbey's and 3.8-77.5% in Beggiatoa medium. Agar diffusion method was used for studing antimicrobial activities of Cyanobacteria strains on 16 different tested bacteria. Only, Synechocystis sp. B6 strain has been shown the antimicrobial activity on Micrococcus flavus and Proteus vulgaris. The inhibition zones were 0.36 mm. and 0.35 mm. respectively. None other cultures have been shown any antimicrobial activities on tested bacteria.

Keywords:

Turkey, cyanobacteria,

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