Alpan CİNEMRE, Alper AŞÇI, Tahir HAZIR, Caner AÇIKADA

Laktik asitin ölçümünde kullanılan bir el analizörünün değerlendirilmesi:lactate scout(+) güvenirliği ve geçerliği

Bu çalışmanın amacı bir el analizörü olan Lactate Scout Plus (LSP)'nin güvenirliğini ve geçerliğini değerlendirmektir. Geçerlik için YSI Sports 1500 analizörü referans yöntem olarak kullanılmıştır. Kan örnekleri şiddeti giderek artan saha testinde toplanmıştır. Güvenirlik için laktat konsantrasyonu(LA) 0.80 -12.80 mM.$L^{-1}$ aralığında değişen n=34 kan örneği LSP analizöründe iki kez ölçülmüştür. Geçerlik için LA 0.60-11.67 mM$L^{-1}$aralığında değişen n = 84 kan örneği aynı anda hem LSP hem de YSI analizöründe ölçülmüştür. Geçerlik kan örneklerinin tümü (0.60 - 11.67 mM.L1), ve sayısal olarak düşük (< 2.5 mM.$L^{-1}$ (n=37), orta (2.5 - 5.0 mM.$L^{-1}$ (n=20) ve yüksek (>5.0 mM.$L^{-1}$) (n=27) konsantrasyonlar için ayrı ayrı değerlendirilmiştir. LSP'nin tekrarlı ölçümlerinde anlamlı fark saptanmamıştır (p>0.05). LSP'nin sınıf içi güvenirlik katsayısı r = 0.998'dir. Tekrarlı ölçümler için tipik hata 0.19 mM.$L^{-1}$ varyasyon katsayısı ~% 5'dir. Orta konsantrasyon aralığındaki hariç LSP'den ölçülen LA değerleri, referans yöntemden ölçülenden önemli derecede farklıdır (p

Evaluation of a handheld lactic acid analyser: reliability and validity of the lactate Scout (+)

The aim of this study was to evaluate the validity and reliability of the Lactate Scout Plus (LSP) hand-held portable lactate analyzer. YSI Sports 1500 analyzer was used for validity as a reference method. Blood samples were collected during an incremental field test. In order to evaluate reliability, 34 blood samples (n= 34) whose lactate consentration values changed (LA) between 0.80-12.80 mM.L-1 were measured two times by LSP. In order to evaluate validity 84 blood samples (n= 84), whose LA concentrations values changes between 0.60-11.67 mM.L-1 were measured at the same time by YS11500 and LSP. Validity was also assessed measuring three samples nominally called low (0.05). Intra-class correlation coefficient of LSP was r=0.998. Typical error for repeated measures and coefficient of variation was 0.19 mM.L-1 and ~% 5, respectively. All LA values which were measured by LSP was statistically different from the reference method except for the moderate LA concentrations (p

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1. Almarwaey OA, Jones AM, Tolfrey K. (2004). Maximal lactate steady state in trained adolescent runners. Journal of Sports Science, 22(2), 215-225.
2. Baldari C, Bonavolontâ V, Emerenziani GP, Gallotta MC, Silva AJ, Guidetti L. (2009). Accuracy, reliability, linearity of Accutrend and Lactate Pro versus EBIO plus analyzer. European Journal of Applied Physiology, 107, 105-111.
3. Billat LV. (1996). Use of blood lactate measurement for prediction of exercise performance and for control of training. Recommendations for long-distance running. Sports Medicine, 22(3), 157-175.
4. Bland JM, Altman DG. (1986). Statistical methods for assess agreement between two methods of clinical measurement. Lancet, 1, 307-310.
5. Bishop D. (2001). Evaluation of the Accusport Lactate Analyser. International Journal of Sports Medicine, 22, 525-530.
6. Brinkert W, Rommes JH, Bakker J. (1999). Lactate measurements in critically ill patients with a hand-held analyser. Intensive Care Medicine, 25, 966 - 969.
7. Buckley JD, Bourdon PC, Woolford SM. (2003). Effect of measuring blood lactate concentrations using different automated lactate analysers on blood lactate transition thresholds. Journal of Science and Medicine in Sport, 6(4), 408-421.
8. Dascombe BJ, Reaburn PRJ, Sirotic AC, Coutts AJ. (2007). The reliability of the i-STAT clinical portable analyser. Journal of Science and Medicine in Sport, 10, 135-140.
9. Davison RCR, Coleman D, Balmer J, Nunn M, Theakston S, Burrows M, et al. (2000). Assessment of blood lactate: Practical evaluation of the Biosen 5030 lactate analyzer. Medicine and Science in Sports and Exercise, 32(1), 243-247.
10. Fell JW, Rayfield JM, Gulbin JP, Gaffney PT. (1998). Evaluation of the Accusport Lactate Analyser. International Journal of Sports Medicine, 19(3), 199-204.
11. Ferasin L, Dodkin SJ, Amodio A., Murray JK, Papasouliotis K. (2007). Evaluation of a Portable Lactate Analyzer (Lactate Scout) in Dogs. American Society for Veterinary Clinical Pathology, 36, 36-39.
12. Hopkins WG. (2000). Measures of reliability in sports medicine and science. Sports Medicine, 30(1), 1-15.
13. Mc Naughton LR, Thompson D, Philips G, Backx K, Crickmore L. (2002). A comparison of the Lactate Pro, Accusport, Analox GM7 and Kodak Ektachem Lactate Analysers in normal, hot and humid conditions. International Journal of Sports Medicine, 23,130-135.
14. Noordally 0, Vincent JL. (1999). Evaluation of a new, rapid lactate analyzer in critical care. Intensive Care Medicine, 25, 508-513.
15. Pfitzinger P, Freedson PS. (1998). The reliability of lactate measurements during exercise. International Journal of Sports Medicine, 19(5), 349-357.
16. Pinnington H. Dawson B. (2001). Examination of the validity and reliability of the Accusport Blood Lactate Analyser. Journal of Science and Medicine in Sport, 4(1), 129-138.
17. Pyne DB, Boston T, Martin DT, Logan A. (2000). Evaluation of the Lactate Pro, blood lactate analyser. European Journal of Applied Physiology, 82,112-116.
18. Seiler, KS, Kjerland GO. (2006). Quantifying training intensity distribution in elite endurance athletes: Is there evidence for an "optimal" distribution? Scandinavian Journal of Medicine and Science in Sports, 16, 49-56.
19. Tanner RK, Fuller KL, Ross ML. (2010). Evaluation of three portable blood lactate analysers: Lactate Pro, Lactate Scout and Lactate Plus. European Journal of Applied Physiology, 109 (3), 551-559.
20. Van Someren KA, Howatson G, Nunan D, Thatcher R, Shave R. (2005). Comparison of the Lactate Pro and Analox GM7 Blood Lactate Analysers. International Journal of Sports Medicine, 26, 657-661.
21. Von Duvillard SP, Pokan R, Hoffman P, Wonisch M, Smekal G, Alkhatib A, et al. (2005). Comparing blood lactate values of three different handheld lactate analyzers to YSI 1500 Lactate Analyzer. Medicine and Science in Sports and Exercise, 37(5), S25.
22. Wigglesworth JK, LaMere VJ, Rowland ND, Miller L. (1996). Examination of the reliability and validity of a new blood lactate analyser. Medicine and Science in Sport and Exercise, 28 (5), 10, (Abstr).