Rahime ASLANKOÇ, Oğuzhan KAVRIK, Özlem ÖZMEN

Elektrik alanın DNA Hasarı ve Beyin Dokusu Üzerine Etkileri - Astaksantin’in Rolü

Özet Bu çalışmada, uzun süreli elektrik alan (EA) maruziyetinin lenfosit DNA hasarı ve beyin dokusu üzerine etkilerini araştırmayı amaçladık. Doğal bir karetonoid pigmenti olan astaksantin’in (AST) EA’nın zararlı etkilerini azaltabileceğini düşündük. Çalışmada, 30 adet Wistar Albino (3-4 aylık) dişi sıçanlar kullanıldı. Sıçanlar her grupta 10 hayvan olmak üzere rastgele üç gruba ayrıldı; Grup I (Kontrol), Grup II (EA uygulanan grup - 10 kV/m EA, 30 gün, günde 23 saat), Grup III (EA+AST tedavi grubu - 10 kV/m EA, 30 gün, günde 23 saat + 100 mg/kg/gün AST, 30 gün, gavaj). Sıçanlar deney sonunda sakrifiye edildi. Kan ve beyin dokusu biyokimyasal analizler için toplandı. Kan dokuda comet assay yöntemi ile lenfosit DNA hasarı analizi, beyin dokuda malondialdehit (MDA) seviyesi, süperoksit dismutaz (SOD) ve katalaz (CAT) enzim aktiviteleri çalışıldı. EA uygulanan grupta kontrol grubuna göre MDA ve comet skoru yüksek bulundu. AST uygulanan grupta EA grubuna göre MDA ve comet skoru azalırken SOD ve CAT enzim aktiviteleri arttı. Çalışma sonuçları EA’ın kan dokuda lenfosit DNA hasarına ve beyin dokuda oksidatif strese neden olduğunu gösterdi. Ayrıca AST tedavisinin lenfosit DNA hasarını ve oksidatif stresi azalttığını gösterdi.

Anahtar Kelimeler:

Elektrik alan, Oksidaitif stres

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